Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04985 and RR42_RS04990 are separated by 174 nucleotides RR42_RS04990 and RR42_RS04995 are separated by 31 nucleotides RR42_RS04995 and RR42_RS05000 are separated by 187 nucleotides
RR42_RS04985: RR42_RS04985 - 2-aminoadipate aminotransferase, at 1,101,362 to 1,102,804
_RS04985
RR42_RS04990: RR42_RS04990 - hypothetical protein, at 1,102,979 to 1,103,344
_RS04990
RR42_RS04995: RR42_RS04995 - single-stranded DNA-binding protein, at 1,103,376 to 1,103,681
_RS04995
RR42_RS05000: RR42_RS05000 - hypothetical protein, at 1,103,869 to 1,105,491
_RS05000
Position (kb)
1102
1103
1104 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1102.060 kb on + strand, within RR42_RS04985 at 1102.068 kb on + strand, within RR42_RS04985 at 1102.069 kb on - strand, within RR42_RS04985 at 1102.069 kb on - strand, within RR42_RS04985 at 1102.069 kb on - strand, within RR42_RS04985 at 1102.176 kb on + strand, within RR42_RS04985 at 1102.176 kb on + strand, within RR42_RS04985 at 1102.177 kb on - strand, within RR42_RS04985 at 1102.177 kb on - strand, within RR42_RS04985 at 1102.177 kb on - strand, within RR42_RS04985 at 1102.179 kb on + strand, within RR42_RS04985 at 1102.179 kb on + strand, within RR42_RS04985 at 1102.180 kb on - strand, within RR42_RS04985 at 1102.180 kb on - strand, within RR42_RS04985 at 1102.180 kb on - strand, within RR42_RS04985 at 1102.273 kb on + strand, within RR42_RS04985 at 1102.274 kb on - strand, within RR42_RS04985 at 1102.527 kb on + strand, within RR42_RS04985 at 1102.527 kb on + strand, within RR42_RS04985 at 1102.527 kb on + strand, within RR42_RS04985 at 1102.528 kb on - strand, within RR42_RS04985 at 1102.764 kb on + strand at 1102.764 kb on + strand at 1102.841 kb on + strand at 1102.841 kb on + strand at 1102.881 kb on - strand at 1102.942 kb on + strand at 1102.942 kb on + strand at 1102.988 kb on + strand at 1102.989 kb on - strand at 1102.989 kb on - strand at 1103.125 kb on + strand, within RR42_RS04990 at 1103.608 kb on + strand, within RR42_RS04995 at 1103.608 kb on + strand, within RR42_RS04995 at 1103.609 kb on - strand, within RR42_RS04995 at 1103.609 kb on - strand, within RR42_RS04995 at 1103.609 kb on - strand, within RR42_RS04995 at 1103.609 kb on - strand, within RR42_RS04995 at 1103.873 kb on + strand at 1103.873 kb on + strand at 1103.873 kb on + strand at 1103.873 kb on + strand at 1103.874 kb on - strand at 1103.874 kb on - strand at 1103.874 kb on - strand at 1103.874 kb on - strand at 1103.879 kb on + strand at 1103.880 kb on - strand at 1104.028 kb on - strand at 1104.029 kb on + strand at 1104.040 kb on + strand, within RR42_RS05000 at 1104.040 kb on + strand, within RR42_RS05000 at 1104.040 kb on + strand, within RR42_RS05000 at 1104.041 kb on - strand, within RR42_RS05000 at 1104.041 kb on - strand, within RR42_RS05000
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,102,060 + RR42_RS04985 0.48 -0.5 1,102,068 + RR42_RS04985 0.49 +0.4 1,102,069 - RR42_RS04985 0.49 -0.0 1,102,069 - RR42_RS04985 0.49 -0.2 1,102,069 - RR42_RS04985 0.49 +1.4 1,102,176 + RR42_RS04985 0.56 +0.1 1,102,176 + RR42_RS04985 0.56 -0.1 1,102,177 - RR42_RS04985 0.56 +1.5 1,102,177 - RR42_RS04985 0.56 +1.2 1,102,177 - RR42_RS04985 0.56 +0.7 1,102,179 + RR42_RS04985 0.57 +0.8 1,102,179 + RR42_RS04985 0.57 -0.1 1,102,180 - RR42_RS04985 0.57 +0.1 1,102,180 - RR42_RS04985 0.57 +0.2 1,102,180 - RR42_RS04985 0.57 +0.5 1,102,273 + RR42_RS04985 0.63 -0.2 1,102,274 - RR42_RS04985 0.63 +0.3 1,102,527 + RR42_RS04985 0.81 -0.5 1,102,527 + RR42_RS04985 0.81 +0.9 1,102,527 + RR42_RS04985 0.81 -1.8 1,102,528 - RR42_RS04985 0.81 -0.3 1,102,764 + -2.5 1,102,764 + -2.3 1,102,841 + -0.9 1,102,841 + -0.9 1,102,881 - -0.6 1,102,942 + -0.1 1,102,942 + +2.0 1,102,988 + -1.1 1,102,989 - +0.2 1,102,989 - +1.5 1,103,125 + RR42_RS04990 0.40 +1.3 1,103,608 + RR42_RS04995 0.76 +0.4 1,103,608 + RR42_RS04995 0.76 +0.2 1,103,609 - RR42_RS04995 0.76 -0.1 1,103,609 - RR42_RS04995 0.76 +0.5 1,103,609 - RR42_RS04995 0.76 +0.7 1,103,609 - RR42_RS04995 0.76 -0.6 1,103,873 + +0.0 1,103,873 + -0.2 1,103,873 + -0.9 1,103,873 + -0.8 1,103,874 - -1.5 1,103,874 - +1.1 1,103,874 - +0.2 1,103,874 - -0.3 1,103,879 + +1.5 1,103,880 - -0.1 1,104,028 - -0.4 1,104,029 + +0.5 1,104,040 + RR42_RS05000 0.11 -1.4 1,104,040 + RR42_RS05000 0.11 -0.8 1,104,040 + RR42_RS05000 0.11 -0.2 1,104,041 - RR42_RS05000 0.11 -1.8 1,104,041 - RR42_RS05000 0.11 +0.5
Or see this region's nucleotide sequence