Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04960 and RR42_RS04965 are separated by 705 nucleotides RR42_RS04965 and RR42_RS04970 are separated by 209 nucleotides
RR42_RS04960: RR42_RS04960 - Fe(II)-dependent oxygenase, at 1,096,205 to 1,096,888
_RS04960
RR42_RS04965: RR42_RS04965 - hypothetical protein, at 1,097,594 to 1,097,905
_RS04965
RR42_RS04970: RR42_RS04970 - aspartyl beta-hydroxylase, at 1,098,115 to 1,099,017
_RS04970
Position (kb)
1097
1098 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1096.612 kb on + strand, within RR42_RS04960 at 1096.616 kb on + strand, within RR42_RS04960 at 1096.616 kb on + strand, within RR42_RS04960 at 1096.616 kb on + strand, within RR42_RS04960 at 1096.617 kb on - strand, within RR42_RS04960 at 1096.617 kb on - strand, within RR42_RS04960 at 1096.651 kb on + strand, within RR42_RS04960 at 1096.651 kb on + strand, within RR42_RS04960 at 1096.662 kb on - strand, within RR42_RS04960 at 1096.854 kb on - strand at 1097.074 kb on + strand at 1097.123 kb on + strand at 1097.123 kb on + strand at 1097.156 kb on + strand at 1097.156 kb on + strand at 1097.157 kb on - strand at 1097.157 kb on - strand at 1097.269 kb on + strand at 1097.270 kb on - strand at 1097.300 kb on + strand at 1097.301 kb on - strand at 1097.301 kb on - strand at 1097.301 kb on - strand at 1097.355 kb on + strand at 1097.356 kb on - strand at 1097.356 kb on - strand at 1097.359 kb on - strand at 1097.378 kb on - strand at 1097.378 kb on - strand at 1097.414 kb on + strand at 1097.421 kb on + strand at 1097.447 kb on + strand at 1097.541 kb on + strand at 1097.541 kb on + strand at 1097.655 kb on - strand, within RR42_RS04965 at 1097.655 kb on - strand, within RR42_RS04965 at 1097.806 kb on + strand, within RR42_RS04965 at 1097.806 kb on + strand, within RR42_RS04965 at 1097.806 kb on + strand, within RR42_RS04965 at 1097.807 kb on - strand, within RR42_RS04965 at 1097.929 kb on + strand at 1097.929 kb on + strand at 1097.930 kb on - strand at 1097.939 kb on - strand at 1097.947 kb on + strand at 1098.031 kb on - strand at 1098.035 kb on + strand at 1098.069 kb on - strand at 1098.069 kb on - strand at 1098.074 kb on + strand at 1098.074 kb on + strand at 1098.100 kb on + strand at 1098.100 kb on + strand at 1098.160 kb on + strand at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.250 kb on + strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.251 kb on - strand, within RR42_RS04970 at 1098.254 kb on + strand, within RR42_RS04970 at 1098.255 kb on - strand, within RR42_RS04970 at 1098.280 kb on + strand, within RR42_RS04970 at 1098.280 kb on + strand, within RR42_RS04970 at 1098.280 kb on + strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.281 kb on - strand, within RR42_RS04970 at 1098.428 kb on - strand, within RR42_RS04970 at 1098.445 kb on + strand, within RR42_RS04970 at 1098.675 kb on + strand, within RR42_RS04970 at 1098.675 kb on + strand, within RR42_RS04970 at 1098.675 kb on + strand, within RR42_RS04970 at 1098.676 kb on - strand, within RR42_RS04970 at 1098.676 kb on - strand, within RR42_RS04970 at 1098.676 kb on - strand, within RR42_RS04970 at 1098.701 kb on + strand, within RR42_RS04970 at 1098.701 kb on + strand, within RR42_RS04970 at 1098.701 kb on - strand, within RR42_RS04970 at 1098.715 kb on + strand, within RR42_RS04970 at 1098.715 kb on + strand, within RR42_RS04970 at 1098.716 kb on - strand, within RR42_RS04970 at 1098.901 kb on - strand, within RR42_RS04970
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,096,612 + RR42_RS04960 0.60 +1.9 1,096,616 + RR42_RS04960 0.60 +0.4 1,096,616 + RR42_RS04960 0.60 +0.3 1,096,616 + RR42_RS04960 0.60 +1.5 1,096,617 - RR42_RS04960 0.60 +0.2 1,096,617 - RR42_RS04960 0.60 -2.3 1,096,651 + RR42_RS04960 0.65 +1.5 1,096,651 + RR42_RS04960 0.65 +1.2 1,096,662 - RR42_RS04960 0.67 +1.5 1,096,854 - +0.6 1,097,074 + +1.3 1,097,123 + +1.5 1,097,123 + -2.8 1,097,156 + +0.3 1,097,156 + -1.0 1,097,157 - -1.0 1,097,157 - -0.9 1,097,269 + -1.1 1,097,270 - +0.3 1,097,300 + -1.5 1,097,301 - +0.1 1,097,301 - -1.1 1,097,301 - +0.3 1,097,355 + +0.2 1,097,356 - -0.9 1,097,356 - +1.1 1,097,359 - -1.4 1,097,378 - -0.1 1,097,378 - -1.4 1,097,414 + +0.2 1,097,421 + +0.3 1,097,447 + -1.9 1,097,541 + +0.5 1,097,541 + -0.4 1,097,655 - RR42_RS04965 0.20 -0.5 1,097,655 - RR42_RS04965 0.20 +0.2 1,097,806 + RR42_RS04965 0.68 -0.3 1,097,806 + RR42_RS04965 0.68 -0.3 1,097,806 + RR42_RS04965 0.68 -0.1 1,097,807 - RR42_RS04965 0.68 -1.4 1,097,929 + +0.5 1,097,929 + -0.1 1,097,930 - -2.0 1,097,939 - -0.1 1,097,947 + +0.9 1,098,031 - -1.7 1,098,035 + +1.3 1,098,069 - -0.8 1,098,069 - -2.4 1,098,074 + -1.9 1,098,074 + +0.6 1,098,100 + -2.0 1,098,100 + -0.7 1,098,160 + -1.0 1,098,250 + RR42_RS04970 0.15 -3.8 1,098,250 + RR42_RS04970 0.15 -0.9 1,098,250 + RR42_RS04970 0.15 -0.8 1,098,250 + RR42_RS04970 0.15 -0.2 1,098,250 + RR42_RS04970 0.15 -1.8 1,098,251 - RR42_RS04970 0.15 -0.6 1,098,251 - RR42_RS04970 0.15 -0.2 1,098,251 - RR42_RS04970 0.15 -2.7 1,098,251 - RR42_RS04970 0.15 -1.1 1,098,251 - RR42_RS04970 0.15 -1.2 1,098,254 + RR42_RS04970 0.15 -0.2 1,098,255 - RR42_RS04970 0.16 -0.7 1,098,280 + RR42_RS04970 0.18 -0.7 1,098,280 + RR42_RS04970 0.18 -2.0 1,098,280 + RR42_RS04970 0.18 -0.6 1,098,281 - RR42_RS04970 0.18 +0.5 1,098,281 - RR42_RS04970 0.18 +0.2 1,098,281 - RR42_RS04970 0.18 -0.8 1,098,281 - RR42_RS04970 0.18 -0.5 1,098,428 - RR42_RS04970 0.35 -1.0 1,098,445 + RR42_RS04970 0.37 -1.7 1,098,675 + RR42_RS04970 0.62 -1.5 1,098,675 + RR42_RS04970 0.62 -2.8 1,098,675 + RR42_RS04970 0.62 -0.5 1,098,676 - RR42_RS04970 0.62 -1.3 1,098,676 - RR42_RS04970 0.62 -1.0 1,098,676 - RR42_RS04970 0.62 +0.9 1,098,701 + RR42_RS04970 0.65 +0.3 1,098,701 + RR42_RS04970 0.65 -0.4 1,098,701 - RR42_RS04970 0.65 -1.3 1,098,715 + RR42_RS04970 0.66 -1.0 1,098,715 + RR42_RS04970 0.66 -1.7 1,098,716 - RR42_RS04970 0.67 -0.3 1,098,901 - RR42_RS04970 0.87 -1.1
Or see this region's nucleotide sequence