Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04845 and RR42_RS04850 are separated by 148 nucleotides RR42_RS04850 and RR42_RS04855 are separated by 25 nucleotides RR42_RS04855 and RR42_RS04860 are separated by 70 nucleotides RR42_RS04860 and RR42_RS04865 are separated by 8 nucleotides
RR42_RS04845: RR42_RS04845 - LacI family transcriptional regulator, at 1,079,218 to 1,080,255
_RS04845
RR42_RS04850: RR42_RS04850 - hypothetical protein, at 1,080,404 to 1,080,778
_RS04850
RR42_RS04855: RR42_RS04855 - hypothetical protein, at 1,080,804 to 1,080,989
_RS04855
RR42_RS04860: RR42_RS04860 - hypothetical protein, at 1,081,060 to 1,081,362
_RS04860
RR42_RS04865: RR42_RS04865 - hypothetical protein, at 1,081,371 to 1,081,748
_RS04865
Position (kb)
1080
1081 Strain fitness (log2 ratio)
-2
-1
0
1 at 1079.543 kb on - strand, within RR42_RS04845 at 1079.572 kb on + strand, within RR42_RS04845 at 1079.608 kb on + strand, within RR42_RS04845 at 1079.716 kb on + strand, within RR42_RS04845 at 1079.833 kb on + strand, within RR42_RS04845 at 1079.834 kb on - strand, within RR42_RS04845 at 1079.834 kb on - strand, within RR42_RS04845 at 1079.920 kb on - strand, within RR42_RS04845 at 1080.028 kb on + strand, within RR42_RS04845 at 1080.029 kb on - strand, within RR42_RS04845 at 1080.253 kb on + strand at 1080.253 kb on + strand at 1080.253 kb on + strand at 1080.254 kb on - strand at 1080.355 kb on + strand at 1080.355 kb on + strand at 1080.356 kb on - strand at 1080.361 kb on - strand at 1080.441 kb on + strand at 1080.600 kb on - strand, within RR42_RS04850 at 1080.713 kb on + strand, within RR42_RS04850 at 1080.714 kb on - strand, within RR42_RS04850 at 1080.743 kb on + strand at 1081.165 kb on + strand, within RR42_RS04860 at 1081.166 kb on - strand, within RR42_RS04860 at 1081.231 kb on - strand, within RR42_RS04860 at 1081.282 kb on + strand, within RR42_RS04860 at 1081.283 kb on - strand, within RR42_RS04860 at 1081.423 kb on + strand, within RR42_RS04865 at 1081.439 kb on + strand, within RR42_RS04865 at 1081.573 kb on + strand, within RR42_RS04865 at 1081.574 kb on - strand, within RR42_RS04865 at 1081.580 kb on - strand, within RR42_RS04865 at 1081.580 kb on - strand, within RR42_RS04865 at 1081.580 kb on - strand, within RR42_RS04865 at 1081.580 kb on - strand, within RR42_RS04865 at 1081.612 kb on + strand, within RR42_RS04865 at 1081.612 kb on + strand, within RR42_RS04865 at 1081.612 kb on + strand, within RR42_RS04865 at 1081.612 kb on + strand, within RR42_RS04865 at 1081.612 kb on + strand, within RR42_RS04865 at 1081.613 kb on - strand, within RR42_RS04865 at 1081.642 kb on + strand, within RR42_RS04865 at 1081.642 kb on + strand, within RR42_RS04865 at 1081.642 kb on + strand, within RR42_RS04865 at 1081.643 kb on - strand, within RR42_RS04865 at 1081.643 kb on - strand, within RR42_RS04865 at 1081.643 kb on - strand, within RR42_RS04865 at 1081.667 kb on - strand, within RR42_RS04865 at 1081.720 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,079,543 - RR42_RS04845 0.31 -1.4 1,079,572 + RR42_RS04845 0.34 +0.5 1,079,608 + RR42_RS04845 0.38 -0.4 1,079,716 + RR42_RS04845 0.48 +0.3 1,079,833 + RR42_RS04845 0.59 -0.5 1,079,834 - RR42_RS04845 0.59 +0.4 1,079,834 - RR42_RS04845 0.59 -0.8 1,079,920 - RR42_RS04845 0.68 +0.0 1,080,028 + RR42_RS04845 0.78 +1.5 1,080,029 - RR42_RS04845 0.78 +0.9 1,080,253 + -0.8 1,080,253 + +0.2 1,080,253 + +0.8 1,080,254 - +0.1 1,080,355 + -1.4 1,080,355 + -1.6 1,080,356 - -0.2 1,080,361 - -0.4 1,080,441 + +0.0 1,080,600 - RR42_RS04850 0.52 -1.1 1,080,713 + RR42_RS04850 0.82 -1.0 1,080,714 - RR42_RS04850 0.83 -1.0 1,080,743 + +0.5 1,081,165 + RR42_RS04860 0.35 -0.6 1,081,166 - RR42_RS04860 0.35 +0.3 1,081,231 - RR42_RS04860 0.56 +0.1 1,081,282 + RR42_RS04860 0.73 +0.5 1,081,283 - RR42_RS04860 0.74 +0.8 1,081,423 + RR42_RS04865 0.14 +0.0 1,081,439 + RR42_RS04865 0.18 +0.2 1,081,573 + RR42_RS04865 0.53 -0.9 1,081,574 - RR42_RS04865 0.54 +1.7 1,081,580 - RR42_RS04865 0.55 -0.2 1,081,580 - RR42_RS04865 0.55 -0.6 1,081,580 - RR42_RS04865 0.55 -1.2 1,081,580 - RR42_RS04865 0.55 -0.5 1,081,612 + RR42_RS04865 0.64 -1.5 1,081,612 + RR42_RS04865 0.64 -1.2 1,081,612 + RR42_RS04865 0.64 -0.1 1,081,612 + RR42_RS04865 0.64 -2.3 1,081,612 + RR42_RS04865 0.64 -0.1 1,081,613 - RR42_RS04865 0.64 -0.8 1,081,642 + RR42_RS04865 0.72 +0.6 1,081,642 + RR42_RS04865 0.72 -1.0 1,081,642 + RR42_RS04865 0.72 -0.2 1,081,643 - RR42_RS04865 0.72 +0.6 1,081,643 - RR42_RS04865 0.72 +0.8 1,081,643 - RR42_RS04865 0.72 +0.6 1,081,667 - RR42_RS04865 0.78 -0.8 1,081,720 - -0.2
Or see this region's nucleotide sequence