Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04630 and RR42_RS04635 are separated by 75 nucleotides RR42_RS04635 and RR42_RS04640 are separated by 143 nucleotides RR42_RS04640 and RR42_RS04645 are separated by 15 nucleotides
RR42_RS04630: RR42_RS04630 - tRNA (guanine-N1)-methyltransferase, at 1,031,016 to 1,031,822
_RS04630
RR42_RS04635: RR42_RS04635 - 50S ribosomal protein L19, at 1,031,898 to 1,032,305
_RS04635
RR42_RS04640: RR42_RS04640 - DNA mismatch repair protein MutT, at 1,032,449 to 1,033,129
_RS04640
RR42_RS04645: RR42_RS04645 - rhodanese superfamily protein, at 1,033,145 to 1,034,002
_RS04645
Position (kb)
1032
1033
1034 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1032.256 kb on - strand, within RR42_RS04635 at 1032.448 kb on + strand at 1032.448 kb on + strand at 1032.448 kb on + strand at 1032.448 kb on + strand at 1032.448 kb on + strand at 1032.448 kb on + strand at 1032.449 kb on - strand at 1032.449 kb on - strand at 1032.449 kb on - strand at 1032.605 kb on + strand, within RR42_RS04640 at 1032.606 kb on - strand, within RR42_RS04640 at 1032.606 kb on - strand, within RR42_RS04640 at 1032.821 kb on + strand, within RR42_RS04640 at 1032.822 kb on - strand, within RR42_RS04640 at 1032.838 kb on + strand, within RR42_RS04640 at 1032.838 kb on + strand, within RR42_RS04640 at 1032.851 kb on + strand, within RR42_RS04640 at 1032.851 kb on + strand, within RR42_RS04640 at 1032.851 kb on + strand, within RR42_RS04640 at 1032.851 kb on + strand, within RR42_RS04640 at 1032.960 kb on + strand, within RR42_RS04640 at 1032.961 kb on - strand, within RR42_RS04640 at 1033.031 kb on + strand, within RR42_RS04640 at 1033.032 kb on - strand, within RR42_RS04640 at 1033.032 kb on - strand, within RR42_RS04640 at 1033.032 kb on - strand, within RR42_RS04640 at 1033.032 kb on - strand, within RR42_RS04640 at 1033.109 kb on + strand at 1033.109 kb on + strand at 1033.110 kb on - strand at 1033.212 kb on + strand at 1033.212 kb on + strand at 1033.212 kb on + strand at 1033.213 kb on - strand at 1033.213 kb on - strand at 1033.311 kb on + strand, within RR42_RS04645 at 1033.339 kb on - strand, within RR42_RS04645 at 1033.365 kb on + strand, within RR42_RS04645 at 1033.366 kb on - strand, within RR42_RS04645 at 1033.393 kb on - strand, within RR42_RS04645 at 1033.977 kb on + strand at 1033.977 kb on + strand at 1033.978 kb on - strand at 1033.978 kb on - strand at 1034.002 kb on + strand at 1034.002 kb on + strand at 1034.003 kb on - strand at 1034.003 kb on - strand at 1034.003 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,032,256 - RR42_RS04635 0.88 +1.1 1,032,448 + +0.2 1,032,448 + +0.5 1,032,448 + -1.5 1,032,448 + +0.4 1,032,448 + +0.4 1,032,448 + -0.4 1,032,449 - -0.3 1,032,449 - +0.2 1,032,449 - -0.2 1,032,605 + RR42_RS04640 0.23 +2.5 1,032,606 - RR42_RS04640 0.23 -1.8 1,032,606 - RR42_RS04640 0.23 -0.4 1,032,821 + RR42_RS04640 0.55 -0.7 1,032,822 - RR42_RS04640 0.55 +0.9 1,032,838 + RR42_RS04640 0.57 -0.3 1,032,838 + RR42_RS04640 0.57 +0.2 1,032,851 + RR42_RS04640 0.59 +0.9 1,032,851 + RR42_RS04640 0.59 -0.8 1,032,851 + RR42_RS04640 0.59 -0.3 1,032,851 + RR42_RS04640 0.59 -1.0 1,032,960 + RR42_RS04640 0.75 +0.2 1,032,961 - RR42_RS04640 0.75 -1.2 1,033,031 + RR42_RS04640 0.85 +0.8 1,033,032 - RR42_RS04640 0.86 -3.0 1,033,032 - RR42_RS04640 0.86 -1.1 1,033,032 - RR42_RS04640 0.86 +0.5 1,033,032 - RR42_RS04640 0.86 -1.5 1,033,109 + -3.9 1,033,109 + -1.8 1,033,110 - -0.2 1,033,212 + -0.0 1,033,212 + -0.3 1,033,212 + -1.0 1,033,213 - +0.9 1,033,213 - +0.7 1,033,311 + RR42_RS04645 0.19 +0.1 1,033,339 - RR42_RS04645 0.23 +0.3 1,033,365 + RR42_RS04645 0.26 +0.9 1,033,366 - RR42_RS04645 0.26 -0.5 1,033,393 - RR42_RS04645 0.29 -0.5 1,033,977 + +0.1 1,033,977 + +0.3 1,033,978 - -0.3 1,033,978 - -1.8 1,034,002 + -0.2 1,034,002 + +0.1 1,034,003 - -0.9 1,034,003 - -0.7 1,034,003 - -1.4
Or see this region's nucleotide sequence