Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS04200 and RR42_RS04205 are separated by 66 nucleotides RR42_RS04205 and RR42_RS04210 are separated by 173 nucleotides
RR42_RS04200: RR42_RS04200 - peptidase M48, at 929,729 to 930,889
_RS04200
RR42_RS04205: RR42_RS04205 - glycine cleavage system regulatory protein, at 930,956 to 931,843
_RS04205
RR42_RS04210: RR42_RS04210 - membrane protein, at 932,017 to 933,306
_RS04210
Position (kb)
930
931
932 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 930.086 kb on + strand, within RR42_RS04200 at 930.092 kb on + strand, within RR42_RS04200 at 930.092 kb on + strand, within RR42_RS04200 at 930.093 kb on - strand, within RR42_RS04200 at 930.509 kb on + strand, within RR42_RS04200 at 930.590 kb on + strand, within RR42_RS04200 at 930.591 kb on - strand, within RR42_RS04200 at 930.887 kb on + strand at 930.887 kb on + strand at 930.887 kb on + strand at 930.888 kb on - strand at 930.888 kb on - strand at 930.888 kb on - strand at 930.888 kb on - strand at 930.910 kb on + strand at 930.910 kb on + strand at 930.910 kb on + strand at 930.910 kb on + strand at 930.911 kb on - strand at 930.911 kb on - strand at 930.911 kb on - strand at 930.976 kb on + strand at 930.976 kb on + strand at 930.977 kb on - strand at 930.977 kb on - strand at 930.977 kb on - strand at 931.032 kb on + strand at 931.033 kb on - strand at 931.033 kb on - strand at 931.093 kb on - strand, within RR42_RS04205 at 931.093 kb on - strand, within RR42_RS04205 at 931.093 kb on - strand, within RR42_RS04205 at 931.411 kb on - strand, within RR42_RS04205 at 931.954 kb on - strand at 931.972 kb on + strand at 932.033 kb on + strand at 932.033 kb on + strand at 932.033 kb on + strand at 932.146 kb on + strand, within RR42_RS04210 at 932.146 kb on + strand, within RR42_RS04210 at 932.146 kb on + strand, within RR42_RS04210 at 932.147 kb on - strand, within RR42_RS04210 at 932.147 kb on - strand, within RR42_RS04210 at 932.148 kb on + strand, within RR42_RS04210 at 932.148 kb on + strand, within RR42_RS04210 at 932.148 kb on + strand, within RR42_RS04210 at 932.149 kb on - strand, within RR42_RS04210 at 932.239 kb on + strand, within RR42_RS04210 at 932.239 kb on + strand, within RR42_RS04210 at 932.240 kb on - strand, within RR42_RS04210 at 932.352 kb on + strand, within RR42_RS04210 at 932.581 kb on + strand, within RR42_RS04210 at 932.581 kb on + strand, within RR42_RS04210 at 932.809 kb on + strand, within RR42_RS04210 at 932.809 kb on + strand, within RR42_RS04210
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 930,086 + RR42_RS04200 0.31 +0.4 930,092 + RR42_RS04200 0.31 -2.3 930,092 + RR42_RS04200 0.31 -1.1 930,093 - RR42_RS04200 0.31 +0.7 930,509 + RR42_RS04200 0.67 +0.3 930,590 + RR42_RS04200 0.74 +0.0 930,591 - RR42_RS04200 0.74 -0.2 930,887 + +0.1 930,887 + -0.6 930,887 + -0.3 930,888 - +0.2 930,888 - -0.6 930,888 - +1.8 930,888 - +0.4 930,910 + +0.1 930,910 + -1.5 930,910 + +0.1 930,910 + +0.2 930,911 - -0.8 930,911 - +0.5 930,911 - +0.2 930,976 + +0.1 930,976 + +0.7 930,977 - +1.0 930,977 - -1.0 930,977 - +0.2 931,032 + +1.8 931,033 - -1.4 931,033 - -0.5 931,093 - RR42_RS04205 0.15 -2.0 931,093 - RR42_RS04205 0.15 -0.0 931,093 - RR42_RS04205 0.15 -1.9 931,411 - RR42_RS04205 0.51 -0.0 931,954 - +0.1 931,972 + -1.3 932,033 + -0.7 932,033 + -3.1 932,033 + -2.3 932,146 + RR42_RS04210 0.10 -1.1 932,146 + RR42_RS04210 0.10 -0.5 932,146 + RR42_RS04210 0.10 +0.5 932,147 - RR42_RS04210 0.10 +0.3 932,147 - RR42_RS04210 0.10 +0.4 932,148 + RR42_RS04210 0.10 -0.4 932,148 + RR42_RS04210 0.10 +0.4 932,148 + RR42_RS04210 0.10 -1.4 932,149 - RR42_RS04210 0.10 -0.7 932,239 + RR42_RS04210 0.17 +0.3 932,239 + RR42_RS04210 0.17 -1.2 932,240 - RR42_RS04210 0.17 -0.3 932,352 + RR42_RS04210 0.26 +0.2 932,581 + RR42_RS04210 0.44 -0.9 932,581 + RR42_RS04210 0.44 -1.2 932,809 + RR42_RS04210 0.61 -0.0 932,809 + RR42_RS04210 0.61 -0.6
Or see this region's nucleotide sequence