Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03435 and RR42_RS03440 are separated by 27 nucleotides RR42_RS03440 and RR42_RS03445 are separated by 179 nucleotides RR42_RS03445 and RR42_RS03450 are separated by 360 nucleotides
RR42_RS03435: RR42_RS03435 - XRE family transcriptional regulator, at 763,992 to 764,801
_RS03435
RR42_RS03440: RR42_RS03440 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 764,829 to 765,587
_RS03440
RR42_RS03445: RR42_RS03445 - adenylate kinase, at 765,767 to 766,432
_RS03445
RR42_RS03450: RR42_RS03450 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 766,793 to 767,602
_RS03450
Position (kb) 765
766
767 Strain fitness (log2 ratio) -2
-1
0
1
2 at 764.831 kb on - strand at 765.115 kb on + strand, within RR42_RS03440 at 765.161 kb on + strand, within RR42_RS03440 at 765.161 kb on + strand, within RR42_RS03440 at 765.161 kb on + strand, within RR42_RS03440 at 765.162 kb on - strand, within RR42_RS03440 at 765.162 kb on - strand, within RR42_RS03440 at 765.162 kb on - strand, within RR42_RS03440 at 765.162 kb on - strand, within RR42_RS03440 at 765.162 kb on - strand, within RR42_RS03440 at 765.162 kb on - strand, within RR42_RS03440 at 765.162 kb on - strand, within RR42_RS03440 at 765.563 kb on + strand at 765.563 kb on + strand at 765.564 kb on - strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.567 kb on + strand at 765.568 kb on - strand at 765.568 kb on - strand at 765.568 kb on - strand at 765.568 kb on - strand at 765.568 kb on - strand at 765.568 kb on - strand at 765.641 kb on - strand at 765.769 kb on - strand at 766.461 kb on - strand at 766.461 kb on - strand at 766.461 kb on - strand at 766.461 kb on - strand at 766.461 kb on - strand at 766.461 kb on - strand at 766.461 kb on - strand at 766.461 kb on - strand at 766.671 kb on - strand at 766.671 kb on - strand at 766.671 kb on - strand at 766.684 kb on - strand at 766.739 kb on + strand at 766.739 kb on + strand at 766.739 kb on + strand at 766.739 kb on + strand at 766.740 kb on - strand at 766.740 kb on - strand at 766.747 kb on - strand at 766.747 kb on - strand at 766.753 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 764,831 - +0.5 765,115 + RR42_RS03440 0.38 +0.0 765,161 + RR42_RS03440 0.44 +0.0 765,161 + RR42_RS03440 0.44 +0.8 765,161 + RR42_RS03440 0.44 +0.0 765,162 - RR42_RS03440 0.44 -0.8 765,162 - RR42_RS03440 0.44 -0.1 765,162 - RR42_RS03440 0.44 -0.6 765,162 - RR42_RS03440 0.44 -0.8 765,162 - RR42_RS03440 0.44 -0.9 765,162 - RR42_RS03440 0.44 -1.8 765,162 - RR42_RS03440 0.44 -1.8 765,563 + -0.8 765,563 + +0.3 765,564 - -0.4 765,567 + +0.4 765,567 + -1.1 765,567 + +0.5 765,567 + -0.8 765,567 + +0.1 765,567 + +0.6 765,567 + +1.4 765,567 + -0.6 765,567 + +0.1 765,568 - -0.2 765,568 - -0.3 765,568 - -0.8 765,568 - -0.3 765,568 - +0.2 765,568 - -0.5 765,641 - +0.3 765,769 - -0.1 766,461 - +1.3 766,461 - -0.5 766,461 - -1.3 766,461 - -0.8 766,461 - +0.3 766,461 - +1.9 766,461 - +1.3 766,461 - -0.4 766,671 - -1.3 766,671 - -0.9 766,671 - +0.1 766,684 - +0.2 766,739 + +0.3 766,739 + -1.2 766,739 + -1.4 766,739 + +0.1 766,740 - +0.7 766,740 - +0.7 766,747 - -0.5 766,747 - -0.2 766,753 + +0.3
Or see this region's nucleotide sequence