Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS03075 and RR42_RS03080 are separated by 310 nucleotides RR42_RS03080 and RR42_RS03085 are separated by 179 nucleotides RR42_RS03085 and RR42_RS03090 are separated by 327 nucleotides
RR42_RS03075: RR42_RS03075 - transcriptional regulator, at 680,385 to 681,071
_RS03075
RR42_RS03080: RR42_RS03080 - recombinase RecA, at 681,382 to 682,455
_RS03080
RR42_RS03085: RR42_RS03085 - RecX family transcriptional regulator, at 682,635 to 683,102
_RS03085
RR42_RS03090: RR42_RS03090 - hypothetical protein, at 683,430 to 684,104
_RS03090
Position (kb) 681
682
683 Strain fitness (log2 ratio) -2
-1
0
1
2 at 680.474 kb on - strand, within RR42_RS03075 at 680.490 kb on + strand, within RR42_RS03075 at 680.490 kb on + strand, within RR42_RS03075 at 680.490 kb on + strand, within RR42_RS03075 at 680.490 kb on + strand, within RR42_RS03075 at 680.491 kb on - strand, within RR42_RS03075 at 680.491 kb on - strand, within RR42_RS03075 at 680.491 kb on - strand, within RR42_RS03075 at 680.494 kb on + strand, within RR42_RS03075 at 680.494 kb on + strand, within RR42_RS03075 at 680.495 kb on - strand, within RR42_RS03075 at 680.495 kb on - strand, within RR42_RS03075 at 680.647 kb on + strand, within RR42_RS03075 at 680.648 kb on - strand, within RR42_RS03075 at 680.779 kb on + strand, within RR42_RS03075 at 680.779 kb on + strand, within RR42_RS03075 at 680.780 kb on - strand, within RR42_RS03075 at 680.780 kb on - strand, within RR42_RS03075 at 680.780 kb on - strand, within RR42_RS03075 at 680.780 kb on - strand, within RR42_RS03075 at 680.780 kb on - strand, within RR42_RS03075 at 680.780 kb on - strand, within RR42_RS03075 at 681.012 kb on + strand at 681.012 kb on + strand at 681.012 kb on + strand at 681.013 kb on - strand at 681.065 kb on + strand at 681.066 kb on - strand at 681.066 kb on - strand at 681.066 kb on - strand at 681.095 kb on + strand at 681.107 kb on + strand at 681.108 kb on - strand at 681.141 kb on + strand at 681.153 kb on - strand at 681.154 kb on + strand at 681.199 kb on + strand at 681.234 kb on - strand at 681.234 kb on - strand at 681.353 kb on - strand at 681.356 kb on + strand at 681.356 kb on + strand at 681.357 kb on - strand at 681.358 kb on + strand at 681.719 kb on - strand, within RR42_RS03080 at 682.164 kb on - strand, within RR42_RS03080 at 682.289 kb on - strand, within RR42_RS03080 at 682.333 kb on + strand, within RR42_RS03080 at 682.555 kb on - strand at 682.564 kb on + strand at 682.564 kb on + strand at 682.567 kb on - strand at 682.567 kb on - strand at 682.568 kb on + strand at 682.569 kb on - strand at 682.569 kb on - strand at 682.569 kb on - strand at 682.569 kb on - strand at 682.577 kb on + strand at 682.577 kb on + strand at 682.580 kb on - strand at 682.591 kb on - strand at 682.591 kb on - strand at 682.592 kb on + strand at 682.592 kb on + strand at 682.592 kb on + strand at 682.593 kb on - strand at 682.593 kb on - strand at 682.593 kb on - strand at 682.594 kb on + strand at 682.594 kb on + strand at 682.595 kb on - strand at 682.595 kb on - strand at 682.595 kb on - strand at 682.680 kb on + strand at 682.680 kb on + strand at 682.681 kb on - strand at 682.845 kb on + strand, within RR42_RS03085 at 683.162 kb on + strand at 683.162 kb on + strand at 683.162 kb on + strand at 683.162 kb on + strand at 683.162 kb on + strand at 683.162 kb on + strand at 683.163 kb on - strand at 683.163 kb on - strand at 683.194 kb on + strand at 683.215 kb on + strand at 683.215 kb on + strand at 683.374 kb on - strand at 683.429 kb on + strand at 683.429 kb on + strand at 683.430 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 680,474 - RR42_RS03075 0.13 -0.1 680,490 + RR42_RS03075 0.15 +0.3 680,490 + RR42_RS03075 0.15 -1.0 680,490 + RR42_RS03075 0.15 +0.5 680,490 + RR42_RS03075 0.15 -0.2 680,491 - RR42_RS03075 0.15 -0.6 680,491 - RR42_RS03075 0.15 -1.4 680,491 - RR42_RS03075 0.15 +0.8 680,494 + RR42_RS03075 0.16 -0.7 680,494 + RR42_RS03075 0.16 -0.5 680,495 - RR42_RS03075 0.16 -0.2 680,495 - RR42_RS03075 0.16 -0.2 680,647 + RR42_RS03075 0.38 +0.2 680,648 - RR42_RS03075 0.38 -0.2 680,779 + RR42_RS03075 0.57 +1.6 680,779 + RR42_RS03075 0.57 +0.1 680,780 - RR42_RS03075 0.57 -0.2 680,780 - RR42_RS03075 0.57 +0.7 680,780 - RR42_RS03075 0.57 -0.3 680,780 - RR42_RS03075 0.57 -0.2 680,780 - RR42_RS03075 0.57 -0.1 680,780 - RR42_RS03075 0.57 -0.6 681,012 + +0.4 681,012 + -2.4 681,012 + -1.7 681,013 - -1.5 681,065 + +0.7 681,066 - +0.2 681,066 - -1.0 681,066 - -0.3 681,095 + -0.1 681,107 + +0.5 681,108 - +1.4 681,141 + +0.6 681,153 - -0.1 681,154 + -0.6 681,199 + +0.3 681,234 - -1.7 681,234 - -0.7 681,353 - -0.9 681,356 + -1.5 681,356 + -0.5 681,357 - -2.7 681,358 + +0.6 681,719 - RR42_RS03080 0.31 +0.6 682,164 - RR42_RS03080 0.73 -1.0 682,289 - RR42_RS03080 0.84 -2.3 682,333 + RR42_RS03080 0.89 -0.6 682,555 - -1.4 682,564 + +0.3 682,564 + -0.6 682,567 - -0.1 682,567 - +0.2 682,568 + -0.2 682,569 - -0.5 682,569 - -1.3 682,569 - -0.7 682,569 - +0.0 682,577 + +0.5 682,577 + -1.0 682,580 - -1.2 682,591 - +1.1 682,591 - +2.6 682,592 + -0.3 682,592 + +0.7 682,592 + -0.7 682,593 - +0.4 682,593 - -0.8 682,593 - +0.5 682,594 + +1.0 682,594 + +0.5 682,595 - -0.2 682,595 - -1.3 682,595 - +0.2 682,680 + +0.1 682,680 + +0.3 682,681 - -1.3 682,845 + RR42_RS03085 0.45 -0.4 683,162 + +1.3 683,162 + +1.6 683,162 + -0.6 683,162 + +0.1 683,162 + -0.4 683,162 + -0.2 683,163 - +0.9 683,163 - -1.0 683,194 + +0.0 683,215 + -0.2 683,215 + +0.0 683,374 - -0.2 683,429 + -0.0 683,429 + -2.6 683,430 - +0.9
Or see this region's nucleotide sequence