Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02460 and RR42_RS02465 are separated by 201 nucleotides RR42_RS02465 and RR42_RS02470 are separated by 105 nucleotides RR42_RS02470 and RR42_RS02475 overlap by 4 nucleotides
RR42_RS02460: RR42_RS02460 - LysR family transcriptional regulator, at 553,879 to 554,775
_RS02460
RR42_RS02465: RR42_RS02465 - membrane protein, at 554,977 to 555,864
_RS02465
RR42_RS02470: RR42_RS02470 - dihydroneopterin triphosphate pyrophosphatase, at 555,970 to 556,443
_RS02470
RR42_RS02475: RR42_RS02475 - endonuclease, at 556,440 to 557,228
_RS02475
Position (kb) 554
555
556 Strain fitness (log2 ratio) -2
-1
0
1
2 at 554.199 kb on + strand, within RR42_RS02460 at 554.199 kb on + strand, within RR42_RS02460 at 554.200 kb on - strand, within RR42_RS02460 at 554.200 kb on - strand, within RR42_RS02460 at 554.347 kb on + strand, within RR42_RS02460 at 554.378 kb on - strand, within RR42_RS02460 at 554.378 kb on - strand, within RR42_RS02460 at 554.378 kb on - strand, within RR42_RS02460 at 554.410 kb on + strand, within RR42_RS02460 at 554.411 kb on - strand, within RR42_RS02460 at 554.498 kb on - strand, within RR42_RS02460 at 554.559 kb on + strand, within RR42_RS02460 at 554.605 kb on + strand, within RR42_RS02460 at 554.605 kb on + strand, within RR42_RS02460 at 554.605 kb on + strand, within RR42_RS02460 at 554.605 kb on + strand, within RR42_RS02460 at 554.680 kb on + strand, within RR42_RS02460 at 554.681 kb on - strand, within RR42_RS02460 at 554.687 kb on + strand at 554.688 kb on - strand at 554.875 kb on + strand at 554.918 kb on - strand at 554.919 kb on + strand at 554.990 kb on + strand at 554.991 kb on - strand at 555.433 kb on + strand, within RR42_RS02465 at 555.433 kb on + strand, within RR42_RS02465 at 555.433 kb on + strand, within RR42_RS02465 at 555.433 kb on + strand, within RR42_RS02465 at 555.433 kb on + strand, within RR42_RS02465 at 555.434 kb on - strand, within RR42_RS02465 at 555.434 kb on - strand, within RR42_RS02465 at 555.516 kb on + strand, within RR42_RS02465 at 555.516 kb on + strand, within RR42_RS02465 at 555.598 kb on + strand, within RR42_RS02465 at 555.623 kb on - strand, within RR42_RS02465 at 555.623 kb on - strand, within RR42_RS02465 at 555.939 kb on + strand at 555.964 kb on - strand at 555.964 kb on - strand at 555.976 kb on + strand at 556.204 kb on + strand, within RR42_RS02470 at 556.204 kb on + strand, within RR42_RS02470 at 556.322 kb on - strand, within RR42_RS02470 at 556.322 kb on - strand, within RR42_RS02470 at 556.439 kb on + strand at 556.439 kb on + strand at 556.461 kb on + strand at 556.462 kb on - strand at 556.632 kb on + strand, within RR42_RS02475 at 556.632 kb on + strand, within RR42_RS02475 at 556.633 kb on - strand, within RR42_RS02475 at 556.684 kb on - strand, within RR42_RS02475 at 556.684 kb on - strand, within RR42_RS02475 at 556.802 kb on + strand, within RR42_RS02475
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 554,199 + RR42_RS02460 0.36 +0.5 554,199 + RR42_RS02460 0.36 -1.4 554,200 - RR42_RS02460 0.36 -0.4 554,200 - RR42_RS02460 0.36 -0.3 554,347 + RR42_RS02460 0.52 -0.8 554,378 - RR42_RS02460 0.56 -0.6 554,378 - RR42_RS02460 0.56 +0.9 554,378 - RR42_RS02460 0.56 -0.9 554,410 + RR42_RS02460 0.59 -0.1 554,411 - RR42_RS02460 0.59 +2.3 554,498 - RR42_RS02460 0.69 +0.3 554,559 + RR42_RS02460 0.76 +0.4 554,605 + RR42_RS02460 0.81 +0.1 554,605 + RR42_RS02460 0.81 +1.6 554,605 + RR42_RS02460 0.81 -1.5 554,605 + RR42_RS02460 0.81 -0.5 554,680 + RR42_RS02460 0.89 -1.4 554,681 - RR42_RS02460 0.89 -0.5 554,687 + +0.1 554,688 - +0.7 554,875 + -1.8 554,918 - -0.2 554,919 + -1.5 554,990 + -1.1 554,991 - -1.3 555,433 + RR42_RS02465 0.51 -0.0 555,433 + RR42_RS02465 0.51 -1.0 555,433 + RR42_RS02465 0.51 -1.7 555,433 + RR42_RS02465 0.51 -0.2 555,433 + RR42_RS02465 0.51 +0.8 555,434 - RR42_RS02465 0.51 -0.0 555,434 - RR42_RS02465 0.51 -1.7 555,516 + RR42_RS02465 0.61 +1.9 555,516 + RR42_RS02465 0.61 +1.9 555,598 + RR42_RS02465 0.70 +0.9 555,623 - RR42_RS02465 0.73 +2.6 555,623 - RR42_RS02465 0.73 -0.5 555,939 + +0.2 555,964 - +0.7 555,964 - -0.1 555,976 + +0.3 556,204 + RR42_RS02470 0.49 +0.6 556,204 + RR42_RS02470 0.49 -1.4 556,322 - RR42_RS02470 0.74 +1.3 556,322 - RR42_RS02470 0.74 +0.3 556,439 + +0.1 556,439 + -2.5 556,461 + -0.2 556,462 - +0.1 556,632 + RR42_RS02475 0.24 -0.2 556,632 + RR42_RS02475 0.24 -1.5 556,633 - RR42_RS02475 0.24 -0.7 556,684 - RR42_RS02475 0.31 -0.4 556,684 - RR42_RS02475 0.31 -1.2 556,802 + RR42_RS02475 0.46 +0.1
Or see this region's nucleotide sequence