Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02325 and RR42_RS02330 are separated by 34 nucleotides RR42_RS02330 and RR42_RS02335 are separated by 14 nucleotides RR42_RS02335 and RR42_RS02340 are separated by 20 nucleotides
RR42_RS02325: RR42_RS02325 - glucoamylase, at 520,917 to 522,755
_RS02325
RR42_RS02330: RR42_RS02330 - trehalose-6-phosphate synthase, at 522,790 to 524,190
_RS02330
RR42_RS02335: RR42_RS02335 - molybdopterin-guanine dinucleotide biosynthesis protein MobA, at 524,205 to 524,810
_RS02335
RR42_RS02340: RR42_RS02340 - lipoprotein, at 524,831 to 525,859
_RS02340
Position (kb) 522
523
524
525 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 521.829 kb on + strand, within RR42_RS02325 at 521.889 kb on + strand, within RR42_RS02325 at 522.010 kb on - strand, within RR42_RS02325 at 522.010 kb on - strand, within RR42_RS02325 at 522.019 kb on + strand, within RR42_RS02325 at 522.019 kb on + strand, within RR42_RS02325 at 522.019 kb on + strand, within RR42_RS02325 at 522.019 kb on + strand, within RR42_RS02325 at 522.085 kb on - strand, within RR42_RS02325 at 522.179 kb on + strand, within RR42_RS02325 at 522.179 kb on + strand, within RR42_RS02325 at 522.179 kb on + strand, within RR42_RS02325 at 522.264 kb on + strand, within RR42_RS02325 at 522.471 kb on + strand, within RR42_RS02325 at 522.471 kb on + strand, within RR42_RS02325 at 522.471 kb on + strand, within RR42_RS02325 at 522.472 kb on - strand, within RR42_RS02325 at 522.472 kb on - strand, within RR42_RS02325 at 522.472 kb on - strand, within RR42_RS02325 at 522.472 kb on - strand, within RR42_RS02325 at 522.473 kb on + strand, within RR42_RS02325 at 522.473 kb on + strand, within RR42_RS02325 at 522.474 kb on - strand, within RR42_RS02325 at 522.474 kb on - strand, within RR42_RS02325 at 522.474 kb on - strand, within RR42_RS02325 at 522.700 kb on + strand at 522.700 kb on + strand at 522.701 kb on - strand at 522.790 kb on - strand at 522.800 kb on + strand at 522.801 kb on - strand at 523.030 kb on + strand, within RR42_RS02330 at 523.034 kb on - strand, within RR42_RS02330 at 523.034 kb on - strand, within RR42_RS02330 at 523.042 kb on + strand, within RR42_RS02330 at 523.075 kb on + strand, within RR42_RS02330 at 523.121 kb on - strand, within RR42_RS02330 at 523.198 kb on + strand, within RR42_RS02330 at 523.269 kb on + strand, within RR42_RS02330 at 523.342 kb on + strand, within RR42_RS02330 at 523.433 kb on - strand, within RR42_RS02330 at 523.540 kb on + strand, within RR42_RS02330 at 523.541 kb on - strand, within RR42_RS02330 at 523.561 kb on + strand, within RR42_RS02330 at 523.562 kb on - strand, within RR42_RS02330 at 523.603 kb on + strand, within RR42_RS02330 at 523.726 kb on + strand, within RR42_RS02330 at 523.727 kb on - strand, within RR42_RS02330 at 523.781 kb on - strand, within RR42_RS02330 at 523.810 kb on + strand, within RR42_RS02330 at 523.962 kb on - strand, within RR42_RS02330 at 524.017 kb on + strand, within RR42_RS02330 at 524.022 kb on + strand, within RR42_RS02330 at 524.023 kb on - strand, within RR42_RS02330 at 524.061 kb on + strand at 524.062 kb on - strand at 524.062 kb on - strand at 524.062 kb on - strand at 524.062 kb on - strand at 524.146 kb on + strand at 524.146 kb on + strand at 524.147 kb on - strand at 524.147 kb on - strand at 524.181 kb on - strand at 524.224 kb on - strand at 524.658 kb on + strand, within RR42_RS02335 at 524.994 kb on + strand, within RR42_RS02340 at 524.994 kb on + strand, within RR42_RS02340 at 524.995 kb on - strand, within RR42_RS02340 at 525.105 kb on + strand, within RR42_RS02340 at 525.106 kb on - strand, within RR42_RS02340 at 525.106 kb on - strand, within RR42_RS02340
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 521,829 + RR42_RS02325 0.50 -1.4 521,889 + RR42_RS02325 0.53 +0.1 522,010 - RR42_RS02325 0.59 -0.1 522,010 - RR42_RS02325 0.59 -0.5 522,019 + RR42_RS02325 0.60 -0.3 522,019 + RR42_RS02325 0.60 +0.7 522,019 + RR42_RS02325 0.60 +0.4 522,019 + RR42_RS02325 0.60 -1.6 522,085 - RR42_RS02325 0.64 -0.1 522,179 + RR42_RS02325 0.69 +0.1 522,179 + RR42_RS02325 0.69 -0.1 522,179 + RR42_RS02325 0.69 -0.1 522,264 + RR42_RS02325 0.73 -0.7 522,471 + RR42_RS02325 0.85 -0.5 522,471 + RR42_RS02325 0.85 +2.6 522,471 + RR42_RS02325 0.85 +0.2 522,472 - RR42_RS02325 0.85 -0.5 522,472 - RR42_RS02325 0.85 +2.6 522,472 - RR42_RS02325 0.85 -2.3 522,472 - RR42_RS02325 0.85 -1.2 522,473 + RR42_RS02325 0.85 +0.7 522,473 + RR42_RS02325 0.85 +1.2 522,474 - RR42_RS02325 0.85 -1.0 522,474 - RR42_RS02325 0.85 +2.6 522,474 - RR42_RS02325 0.85 -0.5 522,700 + +0.4 522,700 + +0.1 522,701 - +0.6 522,790 - -0.3 522,800 + +0.4 522,801 - -1.9 523,030 + RR42_RS02330 0.17 -0.8 523,034 - RR42_RS02330 0.17 -0.6 523,034 - RR42_RS02330 0.17 +0.8 523,042 + RR42_RS02330 0.18 +0.1 523,075 + RR42_RS02330 0.20 +0.1 523,121 - RR42_RS02330 0.24 +1.3 523,198 + RR42_RS02330 0.29 +2.2 523,269 + RR42_RS02330 0.34 -0.4 523,342 + RR42_RS02330 0.39 -1.4 523,433 - RR42_RS02330 0.46 -0.9 523,540 + RR42_RS02330 0.54 -0.1 523,541 - RR42_RS02330 0.54 -0.7 523,561 + RR42_RS02330 0.55 +2.0 523,562 - RR42_RS02330 0.55 -0.3 523,603 + RR42_RS02330 0.58 -0.5 523,726 + RR42_RS02330 0.67 -0.2 523,727 - RR42_RS02330 0.67 -0.6 523,781 - RR42_RS02330 0.71 -0.6 523,810 + RR42_RS02330 0.73 -0.5 523,962 - RR42_RS02330 0.84 -1.2 524,017 + RR42_RS02330 0.88 +0.6 524,022 + RR42_RS02330 0.88 -0.3 524,023 - RR42_RS02330 0.88 -0.2 524,061 + -0.2 524,062 - +0.2 524,062 - -1.9 524,062 - +0.3 524,062 - -0.0 524,146 + -0.3 524,146 + +0.8 524,147 - +3.2 524,147 - -0.2 524,181 - -0.4 524,224 - -0.3 524,658 + RR42_RS02335 0.75 -0.6 524,994 + RR42_RS02340 0.16 +0.1 524,994 + RR42_RS02340 0.16 +0.7 524,995 - RR42_RS02340 0.16 +1.4 525,105 + RR42_RS02340 0.27 -1.0 525,106 - RR42_RS02340 0.27 +0.5 525,106 - RR42_RS02340 0.27 -0.5
Or see this region's nucleotide sequence