Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02220 and RR42_RS02225 are separated by 27 nucleotides RR42_RS02225 and RR42_RS02230 are separated by 67 nucleotides
RR42_RS02220: RR42_RS02220 - LysR family transcriptional regulator, at 491,812 to 492,753
_RS02220
RR42_RS02225: RR42_RS02225 - ABC transporter substrate-binding protein, at 492,781 to 493,764
_RS02225
RR42_RS02230: RR42_RS02230 - sulfolactate dehydrogenase, at 493,832 to 494,836
_RS02230
Position (kb)
492
493
494 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 492.290 kb on + strand, within RR42_RS02220 at 492.290 kb on + strand, within RR42_RS02220 at 492.290 kb on + strand, within RR42_RS02220 at 492.290 kb on + strand, within RR42_RS02220 at 492.290 kb on + strand, within RR42_RS02220 at 492.291 kb on - strand, within RR42_RS02220 at 492.291 kb on - strand, within RR42_RS02220 at 492.292 kb on + strand, within RR42_RS02220 at 492.292 kb on + strand, within RR42_RS02220 at 492.292 kb on + strand, within RR42_RS02220 at 492.292 kb on + strand, within RR42_RS02220 at 492.293 kb on - strand, within RR42_RS02220 at 492.293 kb on - strand, within RR42_RS02220 at 492.293 kb on - strand, within RR42_RS02220 at 492.651 kb on + strand, within RR42_RS02220 at 492.652 kb on - strand, within RR42_RS02220 at 492.652 kb on - strand, within RR42_RS02220 at 492.670 kb on + strand at 492.845 kb on + strand at 493.416 kb on + strand, within RR42_RS02225 at 493.524 kb on + strand, within RR42_RS02225 at 493.525 kb on - strand, within RR42_RS02225 at 493.646 kb on + strand, within RR42_RS02225 at 493.646 kb on + strand, within RR42_RS02225 at 493.739 kb on - strand at 493.845 kb on - strand at 493.992 kb on + strand, within RR42_RS02230 at 493.993 kb on - strand, within RR42_RS02230 at 493.993 kb on - strand, within RR42_RS02230 at 494.048 kb on - strand, within RR42_RS02230 at 494.627 kb on + strand, within RR42_RS02230 at 494.679 kb on + strand, within RR42_RS02230 at 494.679 kb on + strand, within RR42_RS02230 at 494.680 kb on - strand, within RR42_RS02230
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 492,290 + RR42_RS02220 0.51 +0.3 492,290 + RR42_RS02220 0.51 +1.6 492,290 + RR42_RS02220 0.51 +0.6 492,290 + RR42_RS02220 0.51 -0.6 492,290 + RR42_RS02220 0.51 -1.2 492,291 - RR42_RS02220 0.51 -1.0 492,291 - RR42_RS02220 0.51 -0.1 492,292 + RR42_RS02220 0.51 -1.0 492,292 + RR42_RS02220 0.51 -0.1 492,292 + RR42_RS02220 0.51 +1.6 492,292 + RR42_RS02220 0.51 -1.6 492,293 - RR42_RS02220 0.51 +0.5 492,293 - RR42_RS02220 0.51 +0.7 492,293 - RR42_RS02220 0.51 -2.9 492,651 + RR42_RS02220 0.89 -0.5 492,652 - RR42_RS02220 0.89 -0.8 492,652 - RR42_RS02220 0.89 +0.1 492,670 + -0.6 492,845 + -0.3 493,416 + RR42_RS02225 0.65 -1.0 493,524 + RR42_RS02225 0.76 -0.6 493,525 - RR42_RS02225 0.76 +0.6 493,646 + RR42_RS02225 0.88 -1.3 493,646 + RR42_RS02225 0.88 -0.9 493,739 - -1.3 493,845 - -0.9 493,992 + RR42_RS02230 0.16 -0.3 493,993 - RR42_RS02230 0.16 -0.8 493,993 - RR42_RS02230 0.16 -0.4 494,048 - RR42_RS02230 0.21 +0.2 494,627 + RR42_RS02230 0.79 -0.7 494,679 + RR42_RS02230 0.84 -0.0 494,679 + RR42_RS02230 0.84 -0.5 494,680 - RR42_RS02230 0.84 -0.0
Or see this region's nucleotide sequence