Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02050 and RR42_RS02055 are separated by 6 nucleotides RR42_RS02055 and RR42_RS02060 are separated by 66 nucleotides RR42_RS02060 and RR42_RS02065 are separated by 119 nucleotides RR42_RS02065 and RR42_RS02070 are separated by 191 nucleotides
RR42_RS02050: RR42_RS02050 - adenine glycosylase, at 457,498 to 458,667
_RS02050
RR42_RS02055: RR42_RS02055 - ATP-dependent protease, at 458,674 to 459,351
_RS02055
RR42_RS02060: RR42_RS02060 - nucleotide-binding protein, at 459,418 to 460,335
_RS02060
RR42_RS02065: RR42_RS02065 - phosphate starvation-inducible protein PsiF, at 460,455 to 460,778
_RS02065
RR42_RS02070: RR42_RS02070 - HPr kinase, at 460,970 to 461,941
_RS02070
Position (kb) 459
460
461 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 458.463 kb on + strand, within RR42_RS02050 at 458.464 kb on - strand, within RR42_RS02050 at 458.464 kb on - strand, within RR42_RS02050 at 458.464 kb on - strand, within RR42_RS02050 at 458.606 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.699 kb on + strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.700 kb on - strand at 458.703 kb on + strand at 458.704 kb on - strand at 458.704 kb on - strand at 458.704 kb on - strand at 458.704 kb on - strand at 458.881 kb on - strand, within RR42_RS02055 at 459.054 kb on + strand, within RR42_RS02055 at 459.054 kb on + strand, within RR42_RS02055 at 459.054 kb on + strand, within RR42_RS02055 at 459.054 kb on + strand, within RR42_RS02055 at 459.055 kb on - strand, within RR42_RS02055 at 459.055 kb on - strand, within RR42_RS02055 at 459.197 kb on + strand, within RR42_RS02055 at 459.197 kb on + strand, within RR42_RS02055 at 459.198 kb on - strand, within RR42_RS02055 at 459.198 kb on - strand, within RR42_RS02055 at 459.244 kb on + strand, within RR42_RS02055 at 459.245 kb on - strand, within RR42_RS02055 at 459.245 kb on - strand, within RR42_RS02055 at 459.245 kb on - strand, within RR42_RS02055 at 459.249 kb on + strand, within RR42_RS02055 at 459.283 kb on - strand, within RR42_RS02055 at 459.459 kb on + strand at 459.459 kb on + strand at 459.460 kb on - strand at 459.460 kb on - strand at 459.460 kb on - strand at 459.460 kb on - strand at 459.460 kb on - strand at 459.476 kb on + strand at 459.476 kb on + strand at 459.476 kb on + strand at 459.476 kb on + strand at 459.477 kb on - strand at 459.500 kb on + strand at 459.500 kb on + strand at 459.500 kb on + strand at 459.501 kb on - strand at 459.501 kb on - strand at 459.549 kb on - strand, within RR42_RS02060 at 459.549 kb on - strand, within RR42_RS02060 at 459.594 kb on - strand, within RR42_RS02060 at 459.594 kb on - strand, within RR42_RS02060 at 459.601 kb on + strand, within RR42_RS02060 at 459.602 kb on - strand, within RR42_RS02060 at 459.602 kb on - strand, within RR42_RS02060 at 459.602 kb on - strand, within RR42_RS02060 at 459.602 kb on - strand, within RR42_RS02060 at 459.602 kb on - strand, within RR42_RS02060 at 459.695 kb on + strand, within RR42_RS02060 at 459.695 kb on + strand, within RR42_RS02060 at 459.695 kb on + strand, within RR42_RS02060 at 459.696 kb on - strand at 459.696 kb on - strand, within RR42_RS02060 at 459.696 kb on - strand, within RR42_RS02060 at 459.696 kb on - strand, within RR42_RS02060 at 459.699 kb on - strand, within RR42_RS02060 at 459.701 kb on - strand, within RR42_RS02060 at 459.837 kb on + strand, within RR42_RS02060 at 459.837 kb on + strand, within RR42_RS02060 at 459.837 kb on + strand, within RR42_RS02060 at 459.837 kb on + strand, within RR42_RS02060 at 459.838 kb on - strand, within RR42_RS02060 at 459.911 kb on + strand, within RR42_RS02060 at 460.050 kb on - strand, within RR42_RS02060 at 460.050 kb on - strand, within RR42_RS02060 at 460.071 kb on + strand, within RR42_RS02060 at 460.072 kb on - strand, within RR42_RS02060 at 460.209 kb on - strand, within RR42_RS02060 at 460.209 kb on - strand, within RR42_RS02060 at 460.209 kb on - strand, within RR42_RS02060 at 460.260 kb on - strand at 460.276 kb on + strand at 460.276 kb on + strand at 460.277 kb on - strand at 460.277 kb on - strand at 460.277 kb on - strand at 460.277 kb on - strand at 460.320 kb on + strand at 460.320 kb on + strand at 460.320 kb on + strand at 460.321 kb on - strand at 460.321 kb on - strand at 460.321 kb on - strand at 460.321 kb on - strand at 460.324 kb on + strand at 460.325 kb on - strand at 460.325 kb on - strand at 460.325 kb on - strand at 460.325 kb on - strand at 460.325 kb on - strand at 460.325 kb on - strand at 460.325 kb on - strand at 460.325 kb on - strand at 460.335 kb on + strand at 460.335 kb on + strand at 460.349 kb on + strand at 460.349 kb on + strand at 460.350 kb on - strand at 460.391 kb on - strand at 460.423 kb on + strand at 460.451 kb on + strand at 460.456 kb on + strand at 460.456 kb on + strand at 460.456 kb on + strand at 460.456 kb on + strand at 460.456 kb on + strand at 460.457 kb on - strand at 460.457 kb on - strand at 460.577 kb on + strand, within RR42_RS02065 at 460.578 kb on - strand, within RR42_RS02065 at 460.858 kb on + strand at 460.859 kb on - strand at 460.915 kb on + strand at 460.915 kb on + strand at 460.915 kb on + strand at 460.916 kb on - strand at 460.916 kb on - strand at 460.916 kb on - strand at 460.916 kb on - strand at 460.972 kb on - strand at 460.972 kb on - strand at 460.972 kb on - strand at 460.972 kb on - strand at 460.972 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 458,463 + RR42_RS02050 0.82 -0.5 458,464 - RR42_RS02050 0.83 -0.8 458,464 - RR42_RS02050 0.83 +1.2 458,464 - RR42_RS02050 0.83 +0.0 458,606 + -0.4 458,699 + -0.9 458,699 + +0.8 458,699 + +0.7 458,699 + +0.1 458,699 + -1.3 458,699 + +0.6 458,699 + -0.5 458,699 + -1.5 458,699 + +0.9 458,700 - +0.3 458,700 - -2.2 458,700 - -3.0 458,700 - -0.2 458,700 - -0.2 458,700 - +0.2 458,700 - -1.2 458,703 + +0.3 458,704 - -0.2 458,704 - +1.1 458,704 - +2.1 458,704 - -0.2 458,881 - RR42_RS02055 0.31 -1.0 459,054 + RR42_RS02055 0.56 -0.6 459,054 + RR42_RS02055 0.56 -0.2 459,054 + RR42_RS02055 0.56 -0.6 459,054 + RR42_RS02055 0.56 -0.9 459,055 - RR42_RS02055 0.56 +1.9 459,055 - RR42_RS02055 0.56 +0.4 459,197 + RR42_RS02055 0.77 +0.5 459,197 + RR42_RS02055 0.77 +1.6 459,198 - RR42_RS02055 0.77 -1.7 459,198 - RR42_RS02055 0.77 -0.5 459,244 + RR42_RS02055 0.84 +1.1 459,245 - RR42_RS02055 0.84 -0.2 459,245 - RR42_RS02055 0.84 +0.5 459,245 - RR42_RS02055 0.84 +0.3 459,249 + RR42_RS02055 0.85 +0.5 459,283 - RR42_RS02055 0.90 -1.0 459,459 + +1.6 459,459 + -0.8 459,460 - -0.4 459,460 - -0.5 459,460 - -0.5 459,460 - +0.2 459,460 - +1.6 459,476 + -0.7 459,476 + -0.4 459,476 + -0.0 459,476 + -1.3 459,477 - +1.6 459,500 + -1.0 459,500 + +0.1 459,500 + -1.8 459,501 - +1.9 459,501 - +1.6 459,549 - RR42_RS02060 0.14 +0.3 459,549 - RR42_RS02060 0.14 -0.4 459,594 - RR42_RS02060 0.19 +0.2 459,594 - RR42_RS02060 0.19 +1.6 459,601 + RR42_RS02060 0.20 +0.1 459,602 - RR42_RS02060 0.20 -2.7 459,602 - RR42_RS02060 0.20 -0.8 459,602 - RR42_RS02060 0.20 -0.3 459,602 - RR42_RS02060 0.20 +0.3 459,602 - RR42_RS02060 0.20 -0.5 459,695 + RR42_RS02060 0.30 -0.7 459,695 + RR42_RS02060 0.30 -0.5 459,695 + RR42_RS02060 0.30 -0.3 459,696 - +3.6 459,696 - RR42_RS02060 0.30 -1.0 459,696 - RR42_RS02060 0.30 +0.5 459,696 - RR42_RS02060 0.30 -0.1 459,699 - RR42_RS02060 0.31 +0.0 459,701 - RR42_RS02060 0.31 -0.9 459,837 + RR42_RS02060 0.46 +0.8 459,837 + RR42_RS02060 0.46 +0.0 459,837 + RR42_RS02060 0.46 -0.7 459,837 + RR42_RS02060 0.46 +0.1 459,838 - RR42_RS02060 0.46 +0.0 459,911 + RR42_RS02060 0.54 +1.1 460,050 - RR42_RS02060 0.69 -1.3 460,050 - RR42_RS02060 0.69 +0.8 460,071 + RR42_RS02060 0.71 -0.2 460,072 - RR42_RS02060 0.71 -0.2 460,209 - RR42_RS02060 0.86 +0.3 460,209 - RR42_RS02060 0.86 +2.3 460,209 - RR42_RS02060 0.86 -1.5 460,260 - -0.1 460,276 + -0.4 460,276 + -0.6 460,277 - +0.3 460,277 - -0.8 460,277 - -0.4 460,277 - -0.3 460,320 + -0.7 460,320 + -0.9 460,320 + -0.1 460,321 - +0.8 460,321 - +1.0 460,321 - -1.3 460,321 - -0.3 460,324 + +0.6 460,325 - -2.1 460,325 - -0.2 460,325 - -0.4 460,325 - -1.4 460,325 - -0.8 460,325 - +0.3 460,325 - -1.1 460,325 - -0.5 460,335 + -0.1 460,335 + -0.4 460,349 + +0.3 460,349 + +1.2 460,350 - -0.4 460,391 - +0.5 460,423 + -0.1 460,451 + -0.2 460,456 + +0.4 460,456 + -0.5 460,456 + +0.4 460,456 + +0.5 460,456 + +0.1 460,457 - -1.1 460,457 - +0.1 460,577 + RR42_RS02065 0.38 +0.0 460,578 - RR42_RS02065 0.38 +0.7 460,858 + +0.4 460,859 - -0.9 460,915 + -1.2 460,915 + +0.3 460,915 + -0.3 460,916 - -0.4 460,916 - +0.3 460,916 - -0.0 460,916 - -1.7 460,972 - +0.1 460,972 - -0.1 460,972 - -0.1 460,972 - +0.1 460,972 - -1.5
Or see this region's nucleotide sequence