Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01650 and RR42_RS01655 are separated by 160 nucleotides RR42_RS01655 and RR42_RS01660 overlap by 10 nucleotides RR42_RS01660 and RR42_RS01665 are separated by 77 nucleotides
RR42_RS01650: RR42_RS01650 - branched-chain amino acid ABC transporter ATP-binding protein, at 376,432 to 377,247
_RS01650
RR42_RS01655: RR42_RS01655 - AMP-dependent synthetase, at 377,408 to 378,658
_RS01655
RR42_RS01660: RR42_RS01660 - hypothetical protein, at 378,649 to 379,047
_RS01660
RR42_RS01665: RR42_RS01665 - 2-nitropropane dioxygenase, at 379,125 to 380,102
_RS01665
Position (kb) 377
378
379 Strain fitness (log2 ratio) -1
0
1 at 376.456 kb on - strand at 376.468 kb on + strand at 376.468 kb on + strand at 376.469 kb on - strand at 376.469 kb on - strand at 376.760 kb on - strand, within RR42_RS01650 at 376.760 kb on - strand, within RR42_RS01650 at 376.761 kb on + strand, within RR42_RS01650 at 376.761 kb on + strand, within RR42_RS01650 at 376.761 kb on + strand, within RR42_RS01650 at 376.811 kb on - strand, within RR42_RS01650 at 377.060 kb on - strand, within RR42_RS01650 at 377.074 kb on + strand, within RR42_RS01650 at 377.075 kb on - strand, within RR42_RS01650 at 377.152 kb on + strand, within RR42_RS01650 at 377.152 kb on + strand, within RR42_RS01650 at 377.153 kb on - strand, within RR42_RS01650 at 377.218 kb on + strand at 377.218 kb on + strand at 377.218 kb on + strand at 377.370 kb on - strand at 377.392 kb on - strand at 377.400 kb on + strand at 377.401 kb on - strand at 377.417 kb on + strand at 377.517 kb on - strand at 377.786 kb on + strand, within RR42_RS01655 at 377.786 kb on + strand, within RR42_RS01655 at 377.801 kb on + strand, within RR42_RS01655 at 378.089 kb on + strand, within RR42_RS01655 at 378.089 kb on + strand, within RR42_RS01655 at 378.090 kb on - strand, within RR42_RS01655 at 379.107 kb on + strand at 379.162 kb on + strand at 379.357 kb on + strand, within RR42_RS01665 at 379.357 kb on + strand, within RR42_RS01665 at 379.357 kb on + strand, within RR42_RS01665 at 379.357 kb on + strand, within RR42_RS01665 at 379.357 kb on + strand, within RR42_RS01665 at 379.358 kb on - strand, within RR42_RS01665 at 379.358 kb on - strand, within RR42_RS01665 at 379.390 kb on + strand, within RR42_RS01665 at 379.391 kb on - strand, within RR42_RS01665 at 379.391 kb on - strand, within RR42_RS01665 at 379.391 kb on - strand, within RR42_RS01665 at 379.391 kb on - strand, within RR42_RS01665 at 379.391 kb on - strand, within RR42_RS01665
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 376,456 - +0.6 376,468 + -0.7 376,468 + -0.6 376,469 - -0.1 376,469 - -0.7 376,760 - RR42_RS01650 0.40 +1.6 376,760 - RR42_RS01650 0.40 +1.6 376,761 + RR42_RS01650 0.40 +0.0 376,761 + RR42_RS01650 0.40 -0.3 376,761 + RR42_RS01650 0.40 -0.5 376,811 - RR42_RS01650 0.46 +1.0 377,060 - RR42_RS01650 0.77 -0.1 377,074 + RR42_RS01650 0.79 -0.4 377,075 - RR42_RS01650 0.79 -0.4 377,152 + RR42_RS01650 0.88 -0.2 377,152 + RR42_RS01650 0.88 -0.1 377,153 - RR42_RS01650 0.88 +0.9 377,218 + +1.0 377,218 + -0.2 377,218 + +0.6 377,370 - +0.2 377,392 - -0.3 377,400 + -1.4 377,401 - -0.3 377,417 + -0.2 377,517 - -0.1 377,786 + RR42_RS01655 0.30 +1.6 377,786 + RR42_RS01655 0.30 +0.1 377,801 + RR42_RS01655 0.31 +0.6 378,089 + RR42_RS01655 0.54 -0.2 378,089 + RR42_RS01655 0.54 +0.6 378,090 - RR42_RS01655 0.55 -0.7 379,107 + +0.1 379,162 + -0.5 379,357 + RR42_RS01665 0.24 +0.5 379,357 + RR42_RS01665 0.24 -1.0 379,357 + RR42_RS01665 0.24 +0.6 379,357 + RR42_RS01665 0.24 +0.1 379,357 + RR42_RS01665 0.24 +0.1 379,358 - RR42_RS01665 0.24 +0.7 379,358 - RR42_RS01665 0.24 +0.1 379,390 + RR42_RS01665 0.27 -0.8 379,391 - RR42_RS01665 0.27 -0.1 379,391 - RR42_RS01665 0.27 +0.9 379,391 - RR42_RS01665 0.27 +0.4 379,391 - RR42_RS01665 0.27 +0.3 379,391 - RR42_RS01665 0.27 -0.3
Or see this region's nucleotide sequence