Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01505 and RR42_RS01510 are separated by 258 nucleotides RR42_RS01510 and RR42_RS01515 are separated by 85 nucleotides RR42_RS01515 and RR42_RS01520 are separated by 195 nucleotides
RR42_RS01505: RR42_RS01505 - tRNA-Cys, at 340,244 to 340,318
_RS01505
RR42_RS01510: RR42_RS01510 - N-acetylglutamate synthase, at 340,577 to 341,020
_RS01510
RR42_RS01515: RR42_RS01515 - cytidyltransferase, at 341,106 to 342,152
_RS01515
RR42_RS01520: RR42_RS01520 - LysR family transcriptional regulator, at 342,348 to 343,268
_RS01520
Position (kb) 341
342
343 Strain fitness (log2 ratio) -2
-1
0
1 at 340.161 kb on + strand at 340.162 kb on - strand at 340.162 kb on - strand at 340.285 kb on + strand, within RR42_RS01505 at 340.321 kb on - strand at 340.321 kb on - strand at 340.422 kb on - strand at 340.509 kb on + strand at 340.510 kb on - strand at 340.510 kb on - strand at 340.510 kb on - strand at 340.570 kb on - strand at 340.696 kb on + strand, within RR42_RS01510 at 340.697 kb on - strand, within RR42_RS01510 at 340.697 kb on - strand, within RR42_RS01510 at 340.697 kb on - strand, within RR42_RS01510 at 340.780 kb on - strand, within RR42_RS01510 at 340.817 kb on - strand, within RR42_RS01510 at 340.817 kb on - strand, within RR42_RS01510 at 340.896 kb on - strand, within RR42_RS01510 at 340.896 kb on - strand, within RR42_RS01510 at 341.113 kb on - strand at 341.113 kb on - strand at 341.114 kb on + strand at 341.334 kb on + strand, within RR42_RS01515 at 341.335 kb on - strand, within RR42_RS01515 at 341.335 kb on - strand, within RR42_RS01515 at 341.337 kb on + strand, within RR42_RS01515 at 341.338 kb on - strand, within RR42_RS01515 at 341.458 kb on - strand, within RR42_RS01515 at 341.652 kb on + strand, within RR42_RS01515 at 342.186 kb on + strand at 342.279 kb on + strand at 342.280 kb on - strand at 342.359 kb on + strand at 342.361 kb on + strand at 342.362 kb on - strand at 342.362 kb on - strand at 342.362 kb on - strand at 342.362 kb on - strand at 342.558 kb on + strand, within RR42_RS01520 at 342.558 kb on + strand, within RR42_RS01520 at 342.559 kb on - strand, within RR42_RS01520 at 342.631 kb on - strand, within RR42_RS01520 at 342.673 kb on + strand, within RR42_RS01520 at 342.673 kb on + strand, within RR42_RS01520 at 342.673 kb on + strand, within RR42_RS01520 at 342.674 kb on - strand, within RR42_RS01520 at 342.757 kb on + strand, within RR42_RS01520 at 342.758 kb on - strand, within RR42_RS01520 at 342.774 kb on + strand, within RR42_RS01520 at 342.774 kb on + strand, within RR42_RS01520 at 342.775 kb on - strand, within RR42_RS01520 at 342.775 kb on - strand, within RR42_RS01520 at 342.775 kb on - strand, within RR42_RS01520 at 342.775 kb on - strand, within RR42_RS01520 at 343.075 kb on + strand, within RR42_RS01520 at 343.076 kb on - strand, within RR42_RS01520
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 340,161 + -0.1 340,162 - +0.4 340,162 - -2.0 340,285 + RR42_RS01505 0.55 -0.2 340,321 - -1.5 340,321 - +0.3 340,422 - -0.7 340,509 + +0.3 340,510 - -1.1 340,510 - -1.5 340,510 - -0.1 340,570 - +0.6 340,696 + RR42_RS01510 0.27 -0.0 340,697 - RR42_RS01510 0.27 +0.8 340,697 - RR42_RS01510 0.27 +0.6 340,697 - RR42_RS01510 0.27 -0.2 340,780 - RR42_RS01510 0.46 -1.3 340,817 - RR42_RS01510 0.54 +0.2 340,817 - RR42_RS01510 0.54 +1.1 340,896 - RR42_RS01510 0.72 +0.6 340,896 - RR42_RS01510 0.72 +1.3 341,113 - -0.7 341,113 - -2.3 341,114 + +0.9 341,334 + RR42_RS01515 0.22 -0.5 341,335 - RR42_RS01515 0.22 -0.9 341,335 - RR42_RS01515 0.22 -1.3 341,337 + RR42_RS01515 0.22 +1.1 341,338 - RR42_RS01515 0.22 -0.2 341,458 - RR42_RS01515 0.34 -0.1 341,652 + RR42_RS01515 0.52 -2.0 342,186 + +0.2 342,279 + +1.6 342,280 - +0.5 342,359 + -0.1 342,361 + -0.4 342,362 - -1.4 342,362 - +0.0 342,362 - +0.6 342,362 - -0.6 342,558 + RR42_RS01520 0.23 -0.2 342,558 + RR42_RS01520 0.23 +1.0 342,559 - RR42_RS01520 0.23 +0.1 342,631 - RR42_RS01520 0.31 -0.1 342,673 + RR42_RS01520 0.35 -0.8 342,673 + RR42_RS01520 0.35 +0.8 342,673 + RR42_RS01520 0.35 -0.9 342,674 - RR42_RS01520 0.35 +0.1 342,757 + RR42_RS01520 0.44 -0.2 342,758 - RR42_RS01520 0.45 -1.1 342,774 + RR42_RS01520 0.46 -0.4 342,774 + RR42_RS01520 0.46 +0.0 342,775 - RR42_RS01520 0.46 -0.6 342,775 - RR42_RS01520 0.46 +0.6 342,775 - RR42_RS01520 0.46 -0.1 342,775 - RR42_RS01520 0.46 -0.6 343,075 + RR42_RS01520 0.79 -0.9 343,076 - RR42_RS01520 0.79 -0.4
Or see this region's nucleotide sequence