Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01275 and RR42_RS01280 are separated by 293 nucleotides RR42_RS01280 and RR42_RS01285 are separated by 116 nucleotides
RR42_RS01275: RR42_RS01275 - aldehyde dehydrogenase, at 288,247 to 289,659
_RS01275
RR42_RS01280: RR42_RS01280 - GMC family oxidoreductase, at 289,953 to 291,632
_RS01280
RR42_RS01285: RR42_RS01285 - acyl-CoA dehydrogenase, at 291,749 to 293,410
_RS01285
Position (kb) 289
290
291
292 Strain fitness (log2 ratio) -2
-1
0
1
2 at 289.224 kb on - strand, within RR42_RS01275 at 289.292 kb on + strand, within RR42_RS01275 at 289.292 kb on + strand, within RR42_RS01275 at 289.293 kb on - strand, within RR42_RS01275 at 289.293 kb on - strand, within RR42_RS01275 at 289.293 kb on - strand, within RR42_RS01275 at 289.301 kb on + strand, within RR42_RS01275 at 289.302 kb on - strand, within RR42_RS01275 at 289.302 kb on - strand, within RR42_RS01275 at 289.723 kb on + strand at 289.723 kb on + strand at 289.723 kb on + strand at 289.723 kb on + strand at 289.724 kb on - strand at 289.785 kb on + strand at 289.787 kb on + strand at 289.788 kb on - strand at 289.788 kb on - strand at 289.831 kb on + strand at 289.831 kb on + strand at 289.832 kb on - strand at 289.832 kb on - strand at 289.832 kb on - strand at 289.832 kb on - strand at 289.832 kb on - strand at 289.971 kb on + strand at 289.972 kb on - strand at 290.136 kb on - strand, within RR42_RS01280 at 290.136 kb on - strand, within RR42_RS01280 at 290.139 kb on + strand, within RR42_RS01280 at 290.139 kb on + strand, within RR42_RS01280 at 290.139 kb on + strand, within RR42_RS01280 at 290.140 kb on - strand, within RR42_RS01280 at 290.140 kb on - strand, within RR42_RS01280 at 290.140 kb on - strand, within RR42_RS01280 at 290.241 kb on + strand, within RR42_RS01280 at 290.241 kb on + strand, within RR42_RS01280 at 290.241 kb on + strand, within RR42_RS01280 at 290.265 kb on + strand, within RR42_RS01280 at 290.323 kb on - strand, within RR42_RS01280 at 290.819 kb on - strand, within RR42_RS01280 at 290.823 kb on - strand, within RR42_RS01280 at 290.959 kb on + strand, within RR42_RS01280 at 291.095 kb on + strand, within RR42_RS01280 at 291.095 kb on + strand, within RR42_RS01280 at 291.096 kb on - strand, within RR42_RS01280 at 291.096 kb on - strand, within RR42_RS01280 at 291.124 kb on - strand, within RR42_RS01280 at 291.169 kb on + strand, within RR42_RS01280 at 291.170 kb on - strand, within RR42_RS01280 at 291.324 kb on + strand, within RR42_RS01280 at 291.324 kb on + strand, within RR42_RS01280 at 291.325 kb on - strand, within RR42_RS01280 at 291.395 kb on + strand, within RR42_RS01280 at 291.402 kb on + strand, within RR42_RS01280 at 291.402 kb on + strand, within RR42_RS01280 at 291.403 kb on - strand, within RR42_RS01280 at 291.403 kb on - strand, within RR42_RS01280 at 291.403 kb on - strand, within RR42_RS01280 at 291.403 kb on - strand, within RR42_RS01280 at 291.403 kb on - strand, within RR42_RS01280 at 291.403 kb on - strand, within RR42_RS01280 at 291.403 kb on - strand, within RR42_RS01280 at 292.147 kb on - strand, within RR42_RS01285 at 292.176 kb on + strand, within RR42_RS01285 at 292.177 kb on - strand, within RR42_RS01285 at 292.503 kb on + strand, within RR42_RS01285 at 292.503 kb on + strand, within RR42_RS01285 at 292.504 kb on - strand, within RR42_RS01285 at 292.504 kb on - strand, within RR42_RS01285
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 289,224 - RR42_RS01275 0.69 +0.9 289,292 + RR42_RS01275 0.74 -0.6 289,292 + RR42_RS01275 0.74 +0.0 289,293 - RR42_RS01275 0.74 +0.3 289,293 - RR42_RS01275 0.74 -0.4 289,293 - RR42_RS01275 0.74 +0.3 289,301 + RR42_RS01275 0.75 +0.4 289,302 - RR42_RS01275 0.75 +0.9 289,302 - RR42_RS01275 0.75 +1.2 289,723 + +0.3 289,723 + -0.1 289,723 + -0.7 289,723 + -0.8 289,724 - +0.3 289,785 + -0.3 289,787 + +0.1 289,788 - -0.4 289,788 - -1.0 289,831 + +0.7 289,831 + +0.3 289,832 - -0.3 289,832 - -0.3 289,832 - +0.4 289,832 - -0.1 289,832 - -0.8 289,971 + -0.4 289,972 - -0.6 290,136 - RR42_RS01280 0.11 +0.8 290,136 - RR42_RS01280 0.11 -0.8 290,139 + RR42_RS01280 0.11 -0.8 290,139 + RR42_RS01280 0.11 -0.3 290,139 + RR42_RS01280 0.11 -0.4 290,140 - RR42_RS01280 0.11 -0.3 290,140 - RR42_RS01280 0.11 -1.0 290,140 - RR42_RS01280 0.11 -1.1 290,241 + RR42_RS01280 0.17 +1.6 290,241 + RR42_RS01280 0.17 -0.6 290,241 + RR42_RS01280 0.17 +1.3 290,265 + RR42_RS01280 0.19 -1.0 290,323 - RR42_RS01280 0.22 +0.4 290,819 - RR42_RS01280 0.52 +0.2 290,823 - RR42_RS01280 0.52 +0.2 290,959 + RR42_RS01280 0.60 -1.2 291,095 + RR42_RS01280 0.68 -0.3 291,095 + RR42_RS01280 0.68 +0.8 291,096 - RR42_RS01280 0.68 -0.2 291,096 - RR42_RS01280 0.68 +0.1 291,124 - RR42_RS01280 0.70 -0.1 291,169 + RR42_RS01280 0.72 -0.9 291,170 - RR42_RS01280 0.72 -1.6 291,324 + RR42_RS01280 0.82 -0.4 291,324 + RR42_RS01280 0.82 -2.2 291,325 - RR42_RS01280 0.82 -1.1 291,395 + RR42_RS01280 0.86 -0.6 291,402 + RR42_RS01280 0.86 -0.4 291,402 + RR42_RS01280 0.86 -0.3 291,403 - RR42_RS01280 0.86 -0.3 291,403 - RR42_RS01280 0.86 +0.1 291,403 - RR42_RS01280 0.86 +0.1 291,403 - RR42_RS01280 0.86 +2.6 291,403 - RR42_RS01280 0.86 +1.2 291,403 - RR42_RS01280 0.86 +0.3 291,403 - RR42_RS01280 0.86 -0.3 292,147 - RR42_RS01285 0.24 +1.0 292,176 + RR42_RS01285 0.26 +1.2 292,177 - RR42_RS01285 0.26 -1.0 292,503 + RR42_RS01285 0.45 -0.6 292,503 + RR42_RS01285 0.45 +0.1 292,504 - RR42_RS01285 0.45 +0.3 292,504 - RR42_RS01285 0.45 +0.3
Or see this region's nucleotide sequence