Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01250 and RR42_RS01255 are separated by 100 nucleotides RR42_RS01255 and RR42_RS01260 overlap by 4 nucleotides RR42_RS01260 and RR42_RS01265 are separated by 385 nucleotides
RR42_RS01250: RR42_RS01250 - diaminopimelate epimerase, at 282,646 to 283,512
_RS01250
RR42_RS01255: RR42_RS01255 - lauroyl acyltransferase, at 283,613 to 284,506
_RS01255
RR42_RS01260: RR42_RS01260 - lauroyl acyltransferase, at 284,503 to 285,441
_RS01260
RR42_RS01265: RR42_RS01265 - S-adenosylmethionine synthetase, at 285,827 to 286,990
_RS01265
Position (kb) 284
285
286 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 283.523 kb on + strand at 283.523 kb on + strand at 283.617 kb on + strand at 283.617 kb on + strand at 283.669 kb on - strand at 283.672 kb on - strand at 283.785 kb on + strand, within RR42_RS01255 at 283.785 kb on + strand, within RR42_RS01255 at 283.795 kb on - strand, within RR42_RS01255 at 283.795 kb on - strand, within RR42_RS01255 at 283.816 kb on - strand, within RR42_RS01255 at 283.902 kb on + strand, within RR42_RS01255 at 284.075 kb on - strand, within RR42_RS01255 at 284.076 kb on + strand, within RR42_RS01255 at 284.076 kb on + strand, within RR42_RS01255 at 284.077 kb on - strand, within RR42_RS01255 at 284.400 kb on + strand, within RR42_RS01255 at 284.400 kb on + strand, within RR42_RS01255 at 284.652 kb on - strand, within RR42_RS01260 at 284.752 kb on - strand, within RR42_RS01260 at 284.752 kb on - strand, within RR42_RS01260 at 285.057 kb on - strand, within RR42_RS01260 at 285.198 kb on - strand, within RR42_RS01260 at 285.198 kb on - strand, within RR42_RS01260 at 285.198 kb on - strand, within RR42_RS01260 at 285.234 kb on - strand, within RR42_RS01260 at 285.481 kb on - strand at 285.481 kb on - strand at 285.481 kb on - strand at 285.537 kb on - strand at 285.537 kb on - strand at 285.551 kb on - strand at 285.689 kb on - strand at 285.760 kb on + strand at 285.766 kb on + strand at 285.787 kb on + strand at 285.787 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 283,523 + +0.3 283,523 + -1.9 283,617 + +0.5 283,617 + +0.5 283,669 - -3.4 283,672 - -1.6 283,785 + RR42_RS01255 0.19 -2.5 283,785 + RR42_RS01255 0.19 +1.6 283,795 - RR42_RS01255 0.20 -2.9 283,795 - RR42_RS01255 0.20 -1.4 283,816 - RR42_RS01255 0.23 -2.3 283,902 + RR42_RS01255 0.32 -1.0 284,075 - RR42_RS01255 0.52 -2.5 284,076 + RR42_RS01255 0.52 -1.9 284,076 + RR42_RS01255 0.52 -2.5 284,077 - RR42_RS01255 0.52 -1.2 284,400 + RR42_RS01255 0.88 -3.4 284,400 + RR42_RS01255 0.88 -1.7 284,652 - RR42_RS01260 0.16 -2.7 284,752 - RR42_RS01260 0.27 -3.7 284,752 - RR42_RS01260 0.27 -2.0 285,057 - RR42_RS01260 0.59 +0.0 285,198 - RR42_RS01260 0.74 -1.7 285,198 - RR42_RS01260 0.74 -1.9 285,198 - RR42_RS01260 0.74 -3.3 285,234 - RR42_RS01260 0.78 -1.5 285,481 - -0.5 285,481 - +0.3 285,481 - +0.2 285,537 - +1.0 285,537 - +2.6 285,551 - -0.9 285,689 - +1.6 285,760 + -1.8 285,766 + -1.6 285,787 + -1.2 285,787 + -1.4
Or see this region's nucleotide sequence