Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01100 and RR42_RS01105 are separated by 94 nucleotides RR42_RS01105 and RR42_RS01110 are separated by 0 nucleotides RR42_RS01110 and RR42_RS01115 overlap by 4 nucleotides RR42_RS01115 and RR42_RS01120 are separated by 90 nucleotides
RR42_RS01100: RR42_RS01100 - LysR family transcriptional regulator, at 256,406 to 257,341
_RS01100
RR42_RS01105: RR42_RS01105 - membrane protein, at 257,436 to 257,948
_RS01105
RR42_RS01110: RR42_RS01110 - hypothetical protein, at 257,949 to 258,176
_RS01110
RR42_RS01115: RR42_RS01115 - hypothetical protein, at 258,173 to 258,400
_RS01115
RR42_RS01120: RR42_RS01120 - RNA polymerase sigma factor, at 258,491 to 259,072
_RS01120
Position (kb) 257
258
259 Strain fitness (log2 ratio) -2
-1
0
1 at 257.025 kb on + strand, within RR42_RS01100 at 257.026 kb on - strand, within RR42_RS01100 at 257.273 kb on - strand at 257.273 kb on - strand at 257.273 kb on - strand at 257.273 kb on - strand at 257.350 kb on + strand at 257.350 kb on + strand at 257.360 kb on - strand at 257.392 kb on - strand at 257.706 kb on + strand, within RR42_RS01105 at 257.707 kb on - strand, within RR42_RS01105 at 257.707 kb on - strand, within RR42_RS01105 at 257.707 kb on - strand, within RR42_RS01105 at 257.707 kb on - strand, within RR42_RS01105 at 257.712 kb on - strand, within RR42_RS01105 at 257.712 kb on - strand, within RR42_RS01105 at 257.712 kb on - strand, within RR42_RS01105 at 257.757 kb on - strand, within RR42_RS01105 at 258.391 kb on + strand at 258.392 kb on - strand at 258.552 kb on + strand, within RR42_RS01120 at 258.553 kb on - strand, within RR42_RS01120 at 258.553 kb on - strand, within RR42_RS01120 at 258.571 kb on + strand, within RR42_RS01120 at 258.571 kb on + strand, within RR42_RS01120 at 258.572 kb on - strand, within RR42_RS01120 at 258.572 kb on - strand, within RR42_RS01120 at 258.599 kb on + strand, within RR42_RS01120 at 258.620 kb on + strand, within RR42_RS01120 at 258.620 kb on + strand, within RR42_RS01120 at 258.748 kb on + strand, within RR42_RS01120 at 258.749 kb on - strand, within RR42_RS01120 at 258.753 kb on + strand, within RR42_RS01120 at 258.753 kb on + strand, within RR42_RS01120 at 258.753 kb on + strand, within RR42_RS01120 at 258.754 kb on - strand, within RR42_RS01120 at 258.872 kb on - strand, within RR42_RS01120 at 258.872 kb on - strand, within RR42_RS01120 at 258.906 kb on + strand, within RR42_RS01120 at 258.906 kb on + strand, within RR42_RS01120 at 258.906 kb on + strand, within RR42_RS01120 at 258.907 kb on - strand, within RR42_RS01120 at 258.980 kb on - strand, within RR42_RS01120 at 259.114 kb on + strand at 259.114 kb on + strand at 259.114 kb on + strand at 259.114 kb on + strand at 259.115 kb on - strand at 259.115 kb on - strand at 259.115 kb on - strand at 259.115 kb on - strand at 259.115 kb on - strand at 259.158 kb on + strand at 259.158 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 257,025 + RR42_RS01100 0.66 +0.6 257,026 - RR42_RS01100 0.66 +0.4 257,273 - +0.6 257,273 - +0.3 257,273 - -0.6 257,273 - -0.8 257,350 + -1.4 257,350 + +0.3 257,360 - -0.5 257,392 - +1.5 257,706 + RR42_RS01105 0.53 -1.8 257,707 - RR42_RS01105 0.53 +0.8 257,707 - RR42_RS01105 0.53 +0.0 257,707 - RR42_RS01105 0.53 +1.2 257,707 - RR42_RS01105 0.53 +0.7 257,712 - RR42_RS01105 0.54 -1.5 257,712 - RR42_RS01105 0.54 +0.0 257,712 - RR42_RS01105 0.54 -0.2 257,757 - RR42_RS01105 0.63 +0.0 258,391 + +1.7 258,392 - +0.6 258,552 + RR42_RS01120 0.10 -0.3 258,553 - RR42_RS01120 0.11 -0.6 258,553 - RR42_RS01120 0.11 -0.2 258,571 + RR42_RS01120 0.14 -1.0 258,571 + RR42_RS01120 0.14 +0.4 258,572 - RR42_RS01120 0.14 -0.5 258,572 - RR42_RS01120 0.14 -1.3 258,599 + RR42_RS01120 0.19 -0.1 258,620 + RR42_RS01120 0.22 +0.2 258,620 + RR42_RS01120 0.22 -0.7 258,748 + RR42_RS01120 0.44 -1.1 258,749 - RR42_RS01120 0.44 +1.2 258,753 + RR42_RS01120 0.45 +0.3 258,753 + RR42_RS01120 0.45 -0.6 258,753 + RR42_RS01120 0.45 -0.1 258,754 - RR42_RS01120 0.45 -0.4 258,872 - RR42_RS01120 0.65 -0.7 258,872 - RR42_RS01120 0.65 +0.6 258,906 + RR42_RS01120 0.71 -0.1 258,906 + RR42_RS01120 0.71 +0.3 258,906 + RR42_RS01120 0.71 -0.4 258,907 - RR42_RS01120 0.71 -1.0 258,980 - RR42_RS01120 0.84 +1.0 259,114 + -0.6 259,114 + -0.1 259,114 + -1.4 259,114 + -0.6 259,115 - -0.2 259,115 - +0.2 259,115 - -0.5 259,115 - +0.1 259,115 - -1.0 259,158 + -0.2 259,158 + -0.0
Or see this region's nucleotide sequence