Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS00365 and RR42_RS00370 are separated by 13 nucleotides RR42_RS00370 and RR42_RS00375 are separated by 95 nucleotides
RR42_RS00365: RR42_RS00365 - dihydropyrimidinase, at 98,817 to 100,232
_RS00365
RR42_RS00370: RR42_RS00370 - Asp/Glu/hydantoin racemase, at 100,246 to 100,974
_RS00370
RR42_RS00375: RR42_RS00375 - MFS transporter, at 101,070 to 102,368
_RS00375
Position (kb) 100
101 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 99.253 kb on + strand, within RR42_RS00365 at 99.377 kb on - strand, within RR42_RS00365 at 99.377 kb on - strand, within RR42_RS00365 at 99.402 kb on - strand, within RR42_RS00365 at 99.418 kb on + strand, within RR42_RS00365 at 99.425 kb on - strand, within RR42_RS00365 at 99.741 kb on + strand, within RR42_RS00365 at 99.758 kb on - strand, within RR42_RS00365 at 99.766 kb on + strand, within RR42_RS00365 at 99.767 kb on - strand, within RR42_RS00365 at 99.767 kb on - strand, within RR42_RS00365 at 99.784 kb on + strand, within RR42_RS00365 at 99.784 kb on + strand, within RR42_RS00365 at 99.785 kb on - strand, within RR42_RS00365 at 99.862 kb on + strand, within RR42_RS00365 at 100.347 kb on - strand, within RR42_RS00370 at 100.496 kb on + strand, within RR42_RS00370 at 100.497 kb on - strand, within RR42_RS00370 at 100.497 kb on - strand, within RR42_RS00370 at 100.497 kb on - strand, within RR42_RS00370 at 100.554 kb on - strand, within RR42_RS00370 at 100.979 kb on + strand at 100.990 kb on - strand at 100.990 kb on - strand at 100.990 kb on - strand at 101.040 kb on + strand at 101.040 kb on + strand at 101.040 kb on + strand at 101.041 kb on - strand at 101.041 kb on - strand at 101.046 kb on + strand at 101.046 kb on + strand at 101.047 kb on - strand at 101.047 kb on - strand at 101.047 kb on - strand at 101.296 kb on + strand, within RR42_RS00375 at 101.297 kb on - strand, within RR42_RS00375 at 101.368 kb on + strand, within RR42_RS00375 at 101.573 kb on - strand, within RR42_RS00375 at 101.599 kb on + strand, within RR42_RS00375 at 101.653 kb on + strand, within RR42_RS00375 at 101.653 kb on + strand, within RR42_RS00375 at 101.654 kb on - strand, within RR42_RS00375 at 101.729 kb on + strand, within RR42_RS00375 at 101.731 kb on + strand, within RR42_RS00375 at 101.731 kb on + strand, within RR42_RS00375 at 101.732 kb on - strand, within RR42_RS00375 at 101.785 kb on + strand, within RR42_RS00375 at 101.786 kb on - strand, within RR42_RS00375 at 101.786 kb on - strand, within RR42_RS00375 at 101.788 kb on + strand, within RR42_RS00375 at 101.788 kb on + strand, within RR42_RS00375 at 101.788 kb on + strand, within RR42_RS00375
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 99,253 + RR42_RS00365 0.31 -0.7 99,377 - RR42_RS00365 0.40 -1.2 99,377 - RR42_RS00365 0.40 +0.8 99,402 - RR42_RS00365 0.41 +0.1 99,418 + RR42_RS00365 0.42 -1.3 99,425 - RR42_RS00365 0.43 -0.6 99,741 + RR42_RS00365 0.65 +0.8 99,758 - RR42_RS00365 0.66 +0.3 99,766 + RR42_RS00365 0.67 -0.3 99,767 - RR42_RS00365 0.67 +0.3 99,767 - RR42_RS00365 0.67 +0.1 99,784 + RR42_RS00365 0.68 +1.6 99,784 + RR42_RS00365 0.68 -2.8 99,785 - RR42_RS00365 0.68 +0.2 99,862 + RR42_RS00365 0.74 +0.8 100,347 - RR42_RS00370 0.14 -0.4 100,496 + RR42_RS00370 0.34 -0.8 100,497 - RR42_RS00370 0.34 -0.1 100,497 - RR42_RS00370 0.34 -0.6 100,497 - RR42_RS00370 0.34 -0.1 100,554 - RR42_RS00370 0.42 +3.6 100,979 + -0.1 100,990 - -0.7 100,990 - -0.8 100,990 - -0.9 101,040 + -0.2 101,040 + -1.0 101,040 + +1.3 101,041 - +0.0 101,041 - -0.2 101,046 + +0.1 101,046 + -0.3 101,047 - +0.6 101,047 - +1.0 101,047 - -0.6 101,296 + RR42_RS00375 0.17 -0.6 101,297 - RR42_RS00375 0.17 -0.9 101,368 + RR42_RS00375 0.23 -0.8 101,573 - RR42_RS00375 0.39 -0.9 101,599 + RR42_RS00375 0.41 -1.7 101,653 + RR42_RS00375 0.45 +1.6 101,653 + RR42_RS00375 0.45 -1.9 101,654 - RR42_RS00375 0.45 +0.6 101,729 + RR42_RS00375 0.51 -0.1 101,731 + RR42_RS00375 0.51 -0.4 101,731 + RR42_RS00375 0.51 +0.5 101,732 - RR42_RS00375 0.51 -0.7 101,785 + RR42_RS00375 0.55 -0.0 101,786 - RR42_RS00375 0.55 -0.6 101,786 - RR42_RS00375 0.55 +0.9 101,788 + RR42_RS00375 0.55 -1.5 101,788 + RR42_RS00375 0.55 -1.1 101,788 + RR42_RS00375 0.55 -0.4
Or see this region's nucleotide sequence