Experiment: Adenine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5867 and Pf1N1B4_5868 are separated by 105 nucleotides Pf1N1B4_5868 and Pf1N1B4_5869 overlap by 4 nucleotides Pf1N1B4_5869 and Pf1N1B4_5870 are separated by 17 nucleotides Pf1N1B4_5870 and Pf1N1B4_5871 are separated by 37 nucleotides Pf1N1B4_5871 and Pf1N1B4_5872 overlap by 4 nucleotides
Pf1N1B4_5867 - hypothetical protein, at 302,569 to 302,763
_5867
Pf1N1B4_5868 - Secreted protein Hcp, at 302,869 to 303,384
_5868
Pf1N1B4_5869 - hypothetical protein, at 303,381 to 303,497
_5869
Pf1N1B4_5870 - VgrG protein, at 303,515 to 305,557
_5870
Pf1N1B4_5871 - FIG00963848: hypothetical protein, at 305,595 to 306,290
_5871
Pf1N1B4_5872 - Rhs family protein, at 306,287 to 311,062
_5872
Position (kb) 303
304
305
306 Strain fitness (log2 ratio) -1
0
1
2 at 302.642 kb on - strand, within Pf1N1B4_5867 at 302.776 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.884 kb on + strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.885 kb on - strand at 302.960 kb on - strand, within Pf1N1B4_5868 at 303.059 kb on + strand, within Pf1N1B4_5868 at 303.089 kb on - strand at 303.196 kb on + strand at 303.197 kb on - strand, within Pf1N1B4_5868 at 303.197 kb on - strand, within Pf1N1B4_5868 at 303.197 kb on - strand, within Pf1N1B4_5868 at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.199 kb on + strand at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.199 kb on + strand, within Pf1N1B4_5868 at 303.200 kb on - strand, within Pf1N1B4_5868 at 303.338 kb on + strand at 303.339 kb on - strand at 303.383 kb on - strand at 303.810 kb on - strand, within Pf1N1B4_5870 at 303.851 kb on - strand, within Pf1N1B4_5870 at 304.268 kb on + strand, within Pf1N1B4_5870 at 304.878 kb on - strand, within Pf1N1B4_5870 at 304.878 kb on - strand, within Pf1N1B4_5870 at 305.108 kb on + strand, within Pf1N1B4_5870 at 305.144 kb on + strand, within Pf1N1B4_5870 at 305.485 kb on + strand at 305.485 kb on + strand at 305.489 kb on - strand at 305.930 kb on + strand, within Pf1N1B4_5871 at 305.930 kb on + strand, within Pf1N1B4_5871 at 305.930 kb on + strand, within Pf1N1B4_5871 at 305.995 kb on - strand, within Pf1N1B4_5871 at 306.002 kb on + strand, within Pf1N1B4_5871 at 306.165 kb on + strand, within Pf1N1B4_5871 at 306.165 kb on + strand, within Pf1N1B4_5871 at 306.286 kb on + strand at 306.287 kb on - strand at 306.407 kb on + strand at 306.497 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine (N) remove 302,642 - Pf1N1B4_5867 0.37 +0.3 302,776 + +0.1 302,884 + +1.5 302,884 + -1.1 302,884 + -1.5 302,884 + -0.1 302,884 + +1.1 302,884 + -0.5 302,884 + -0.5 302,884 + -0.9 302,885 - +0.5 302,885 - -1.5 302,885 - +1.5 302,885 - +0.5 302,885 - -1.1 302,885 - +1.5 302,885 - +0.9 302,885 - +0.5 302,885 - +0.3 302,960 - Pf1N1B4_5868 0.18 -0.7 303,059 + Pf1N1B4_5868 0.37 -0.5 303,089 - +0.5 303,196 + -0.5 303,197 - Pf1N1B4_5868 0.64 +1.5 303,197 - Pf1N1B4_5868 0.64 -0.5 303,197 - Pf1N1B4_5868 0.64 +1.5 303,199 + Pf1N1B4_5868 0.64 -0.1 303,199 + Pf1N1B4_5868 0.64 +1.9 303,199 + -0.5 303,199 + Pf1N1B4_5868 0.64 +0.5 303,199 + Pf1N1B4_5868 0.64 -1.5 303,200 - Pf1N1B4_5868 0.64 +1.5 303,338 + -0.5 303,339 - -0.5 303,383 - +1.5 303,810 - Pf1N1B4_5870 0.14 +0.5 303,851 - Pf1N1B4_5870 0.16 +0.9 304,268 + Pf1N1B4_5870 0.37 +1.9 304,878 - Pf1N1B4_5870 0.67 +0.5 304,878 - Pf1N1B4_5870 0.67 +0.5 305,108 + Pf1N1B4_5870 0.78 +0.1 305,144 + Pf1N1B4_5870 0.80 +0.1 305,485 + +0.1 305,485 + -0.5 305,489 - +1.1 305,930 + Pf1N1B4_5871 0.48 -0.5 305,930 + Pf1N1B4_5871 0.48 +0.5 305,930 + Pf1N1B4_5871 0.48 -0.5 305,995 - Pf1N1B4_5871 0.57 -1.5 306,002 + Pf1N1B4_5871 0.58 +0.5 306,165 + Pf1N1B4_5871 0.82 -0.5 306,165 + Pf1N1B4_5871 0.82 +0.5 306,286 + +2.3 306,287 - -1.1 306,407 + +0.8 306,497 + +0.9
Or see this region's nucleotide sequence