Experiment: Adenine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4768 and Pf1N1B4_4769 are separated by 253 nucleotides Pf1N1B4_4769 and Pf1N1B4_4770 are separated by 118 nucleotides Pf1N1B4_4770 and Pf1N1B4_4771 are separated by 101 nucleotides Pf1N1B4_4771 and Pf1N1B4_4772 are separated by 190 nucleotides
Pf1N1B4_4768 - hypothetical protein, at 5,264,824 to 5,267,631
_4768
Pf1N1B4_4769 - hypothetical protein, at 5,267,885 to 5,268,076
_4769
Pf1N1B4_4770 - Putative autotransporter, at 5,268,195 to 5,270,408
_4770
Pf1N1B4_4771 - Probable transmembrane protein, at 5,270,510 to 5,271,151
_4771
Pf1N1B4_4772 - RND efflux system, inner membrane transporter CmeB, at 5,271,342 to 5,274,440
_4772
Position (kb)
5268
5269
5270
5271 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5267.257 kb on + strand, within Pf1N1B4_4768 at 5267.274 kb on + strand, within Pf1N1B4_4768 at 5267.288 kb on + strand, within Pf1N1B4_4768 at 5267.362 kb on - strand at 5267.413 kb on + strand at 5267.413 kb on + strand at 5267.413 kb on + strand at 5267.418 kb on + strand at 5267.418 kb on + strand at 5267.453 kb on + strand at 5267.453 kb on + strand at 5267.515 kb on + strand at 5267.523 kb on + strand at 5267.523 kb on + strand at 5267.523 kb on + strand at 5267.634 kb on - strand at 5267.795 kb on - strand at 5267.850 kb on + strand at 5267.959 kb on - strand, within Pf1N1B4_4769 at 5268.275 kb on + strand at 5268.275 kb on + strand at 5268.276 kb on - strand at 5268.276 kb on - strand at 5268.508 kb on + strand, within Pf1N1B4_4770 at 5268.710 kb on + strand, within Pf1N1B4_4770 at 5268.711 kb on - strand, within Pf1N1B4_4770 at 5268.711 kb on - strand, within Pf1N1B4_4770 at 5268.828 kb on - strand at 5268.831 kb on - strand, within Pf1N1B4_4770 at 5268.831 kb on - strand, within Pf1N1B4_4770 at 5268.843 kb on - strand, within Pf1N1B4_4770 at 5268.847 kb on + strand, within Pf1N1B4_4770 at 5268.847 kb on + strand, within Pf1N1B4_4770 at 5268.847 kb on + strand, within Pf1N1B4_4770 at 5268.848 kb on - strand, within Pf1N1B4_4770 at 5268.848 kb on - strand, within Pf1N1B4_4770 at 5268.848 kb on - strand, within Pf1N1B4_4770 at 5268.848 kb on - strand, within Pf1N1B4_4770 at 5269.184 kb on + strand, within Pf1N1B4_4770 at 5269.185 kb on - strand, within Pf1N1B4_4770 at 5269.383 kb on - strand, within Pf1N1B4_4770 at 5269.422 kb on + strand, within Pf1N1B4_4770 at 5269.642 kb on - strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.947 kb on + strand, within Pf1N1B4_4770 at 5269.948 kb on - strand, within Pf1N1B4_4770 at 5269.991 kb on + strand, within Pf1N1B4_4770 at 5270.010 kb on + strand, within Pf1N1B4_4770 at 5270.010 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.029 kb on + strand, within Pf1N1B4_4770 at 5270.035 kb on + strand, within Pf1N1B4_4770 at 5270.267 kb on + strand at 5270.315 kb on + strand at 5270.315 kb on + strand at 5270.315 kb on + strand at 5270.386 kb on + strand at 5270.386 kb on + strand at 5270.386 kb on + strand at 5270.386 kb on + strand at 5270.397 kb on + strand at 5270.398 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.561 kb on - strand at 5270.745 kb on + strand, within Pf1N1B4_4771 at 5270.745 kb on + strand at 5270.746 kb on - strand, within Pf1N1B4_4771 at 5270.748 kb on + strand, within Pf1N1B4_4771 at 5270.748 kb on + strand at 5270.749 kb on - strand, within Pf1N1B4_4771 at 5270.749 kb on - strand at 5270.760 kb on + strand, within Pf1N1B4_4771 at 5270.761 kb on - strand, within Pf1N1B4_4771 at 5270.927 kb on + strand at 5270.927 kb on + strand at 5270.927 kb on + strand, within Pf1N1B4_4771 at 5271.177 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine (N) remove 5,267,257 + Pf1N1B4_4768 0.87 +0.4 5,267,274 + Pf1N1B4_4768 0.87 -0.6 5,267,288 + Pf1N1B4_4768 0.88 +2.2 5,267,362 - -0.2 5,267,413 + -1.0 5,267,413 + +0.8 5,267,413 + -1.1 5,267,418 + -0.6 5,267,418 + +0.2 5,267,453 + +0.0 5,267,453 + +1.0 5,267,515 + +0.4 5,267,523 + +1.4 5,267,523 + -0.6 5,267,523 + -0.6 5,267,634 - +0.0 5,267,795 - +0.4 5,267,850 + -0.6 5,267,959 - Pf1N1B4_4769 0.39 -0.6 5,268,275 + +1.0 5,268,275 + -1.6 5,268,276 - -2.1 5,268,276 - -0.6 5,268,508 + Pf1N1B4_4770 0.14 -3.7 5,268,710 + Pf1N1B4_4770 0.23 +0.0 5,268,711 - Pf1N1B4_4770 0.23 -1.6 5,268,711 - Pf1N1B4_4770 0.23 +0.4 5,268,828 - -1.6 5,268,831 - Pf1N1B4_4770 0.29 -0.2 5,268,831 - Pf1N1B4_4770 0.29 +0.8 5,268,843 - Pf1N1B4_4770 0.29 +0.4 5,268,847 + Pf1N1B4_4770 0.29 +1.0 5,268,847 + Pf1N1B4_4770 0.29 -1.0 5,268,847 + Pf1N1B4_4770 0.29 -0.6 5,268,848 - Pf1N1B4_4770 0.29 -0.1 5,268,848 - Pf1N1B4_4770 0.29 -0.6 5,268,848 - Pf1N1B4_4770 0.29 -0.6 5,268,848 - Pf1N1B4_4770 0.29 +0.4 5,269,184 + Pf1N1B4_4770 0.45 -1.6 5,269,185 - Pf1N1B4_4770 0.45 -0.8 5,269,383 - Pf1N1B4_4770 0.54 -1.6 5,269,422 + Pf1N1B4_4770 0.55 -1.6 5,269,642 - Pf1N1B4_4770 0.65 +0.4 5,269,947 + Pf1N1B4_4770 0.79 +0.0 5,269,947 + Pf1N1B4_4770 0.79 -1.3 5,269,947 + Pf1N1B4_4770 0.79 -0.6 5,269,947 + Pf1N1B4_4770 0.79 +1.2 5,269,948 - Pf1N1B4_4770 0.79 +0.4 5,269,991 + Pf1N1B4_4770 0.81 -0.6 5,270,010 + Pf1N1B4_4770 0.82 +1.0 5,270,010 + Pf1N1B4_4770 0.82 +1.8 5,270,029 + Pf1N1B4_4770 0.83 -0.6 5,270,029 + -0.6 5,270,029 + Pf1N1B4_4770 0.83 +0.8 5,270,029 + Pf1N1B4_4770 0.83 +1.4 5,270,029 + Pf1N1B4_4770 0.83 -0.6 5,270,035 + Pf1N1B4_4770 0.83 -0.6 5,270,267 + +0.4 5,270,315 + +0.4 5,270,315 + +0.0 5,270,315 + -0.6 5,270,386 + -0.8 5,270,386 + +2.2 5,270,386 + -0.2 5,270,386 + +0.4 5,270,397 + -0.2 5,270,398 - +1.3 5,270,561 - -0.6 5,270,561 - -0.6 5,270,561 - +0.9 5,270,561 - +1.0 5,270,561 - +0.8 5,270,745 + Pf1N1B4_4771 0.37 +0.4 5,270,745 + -0.6 5,270,746 - Pf1N1B4_4771 0.37 -1.6 5,270,748 + Pf1N1B4_4771 0.37 -1.6 5,270,748 + +0.4 5,270,749 - Pf1N1B4_4771 0.37 -0.6 5,270,749 - -0.6 5,270,760 + Pf1N1B4_4771 0.39 -0.6 5,270,761 - Pf1N1B4_4771 0.39 +0.4 5,270,927 + -1.6 5,270,927 + +1.0 5,270,927 + Pf1N1B4_4771 0.65 -0.0 5,271,177 - +0.8
Or see this region's nucleotide sequence