Experiment: Adenine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_40 and Pf1N1B4_41 are separated by 367 nucleotides Pf1N1B4_41 and Pf1N1B4_42 overlap by 4 nucleotides Pf1N1B4_42 and Pf1N1B4_43 are separated by 327 nucleotides
Pf1N1B4_40 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 46,683 to 48,254
_40
Pf1N1B4_41 - hypothetical protein, at 48,622 to 49,104
_41
Pf1N1B4_42 - hypothetical protein, at 49,101 to 50,606
_42
Pf1N1B4_43 - hypothetical protein, at 50,934 to 51,638
_43
Position (kb)
49
50
51 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 48.134 kb on - strand at 48.249 kb on - strand at 48.273 kb on + strand at 48.302 kb on - strand at 48.316 kb on + strand at 48.316 kb on + strand at 48.338 kb on + strand at 48.338 kb on + strand at 48.353 kb on + strand at 48.382 kb on + strand at 48.382 kb on + strand at 48.424 kb on + strand at 48.424 kb on + strand at 48.425 kb on - strand at 48.425 kb on - strand at 48.429 kb on - strand at 48.458 kb on - strand at 48.507 kb on + strand at 48.507 kb on + strand at 48.589 kb on + strand at 48.589 kb on + strand at 48.590 kb on - strand at 48.627 kb on + strand at 48.627 kb on + strand at 48.627 kb on + strand at 48.628 kb on - strand at 48.727 kb on + strand, within Pf1N1B4_41 at 48.727 kb on + strand, within Pf1N1B4_41 at 48.727 kb on + strand, within Pf1N1B4_41 at 48.728 kb on - strand, within Pf1N1B4_41 at 48.736 kb on + strand, within Pf1N1B4_41 at 48.737 kb on - strand, within Pf1N1B4_41 at 48.737 kb on - strand, within Pf1N1B4_41 at 49.213 kb on + strand at 49.214 kb on - strand at 49.214 kb on - strand at 49.412 kb on + strand, within Pf1N1B4_42 at 49.477 kb on + strand at 49.478 kb on - strand, within Pf1N1B4_42 at 49.553 kb on + strand, within Pf1N1B4_42 at 49.726 kb on + strand, within Pf1N1B4_42 at 49.726 kb on + strand, within Pf1N1B4_42 at 49.726 kb on + strand, within Pf1N1B4_42 at 49.726 kb on + strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.727 kb on - strand, within Pf1N1B4_42 at 49.814 kb on - strand, within Pf1N1B4_42 at 49.829 kb on - strand, within Pf1N1B4_42 at 49.870 kb on + strand, within Pf1N1B4_42 at 49.870 kb on + strand, within Pf1N1B4_42 at 49.984 kb on + strand, within Pf1N1B4_42 at 49.984 kb on + strand, within Pf1N1B4_42 at 49.985 kb on - strand, within Pf1N1B4_42 at 49.999 kb on + strand, within Pf1N1B4_42 at 49.999 kb on + strand, within Pf1N1B4_42 at 50.000 kb on - strand, within Pf1N1B4_42 at 50.000 kb on - strand at 50.212 kb on + strand at 50.212 kb on + strand, within Pf1N1B4_42 at 50.213 kb on - strand, within Pf1N1B4_42 at 50.412 kb on - strand, within Pf1N1B4_42 at 50.595 kb on - strand at 50.710 kb on + strand at 50.841 kb on + strand at 50.904 kb on - strand at 50.959 kb on + strand at 50.987 kb on + strand at 50.987 kb on + strand at 50.987 kb on + strand at 51.128 kb on - strand at 51.142 kb on - strand, within Pf1N1B4_43 at 51.318 kb on + strand, within Pf1N1B4_43 at 51.318 kb on + strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.319 kb on - strand, within Pf1N1B4_43 at 51.417 kb on + strand, within Pf1N1B4_43 at 51.418 kb on - strand at 51.418 kb on - strand, within Pf1N1B4_43 at 51.543 kb on + strand, within Pf1N1B4_43 at 51.543 kb on + strand, within Pf1N1B4_43 at 51.544 kb on - strand, within Pf1N1B4_43 at 51.579 kb on + strand at 51.580 kb on - strand at 51.580 kb on - strand at 51.580 kb on - strand at 51.580 kb on - strand at 51.580 kb on - strand at 51.580 kb on - strand at 51.581 kb on + strand at 51.582 kb on - strand at 51.582 kb on - strand at 51.582 kb on - strand at 51.582 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine (N) remove 48,134 - +1.3 48,249 - +1.5 48,273 + +2.1 48,302 - +0.5 48,316 + +0.3 48,316 + -0.3 48,338 + +1.5 48,338 + +0.7 48,353 + -0.3 48,382 + +0.7 48,382 + -0.3 48,424 + +0.7 48,424 + +2.1 48,425 - -0.8 48,425 - -0.3 48,429 - -0.3 48,458 - -0.0 48,507 + +0.3 48,507 + +0.4 48,589 + +1.3 48,589 + -1.3 48,590 - +1.1 48,627 + +0.7 48,627 + -1.3 48,627 + -0.3 48,628 - +1.7 48,727 + Pf1N1B4_41 0.22 +0.7 48,727 + Pf1N1B4_41 0.22 -0.8 48,727 + Pf1N1B4_41 0.22 +0.7 48,728 - Pf1N1B4_41 0.22 -0.3 48,736 + Pf1N1B4_41 0.24 +0.2 48,737 - Pf1N1B4_41 0.24 +0.3 48,737 - Pf1N1B4_41 0.24 +1.3 49,213 + -0.8 49,214 - +2.1 49,214 - +1.7 49,412 + Pf1N1B4_42 0.21 +1.7 49,477 + -0.3 49,478 - Pf1N1B4_42 0.25 +2.1 49,553 + Pf1N1B4_42 0.30 -1.3 49,726 + Pf1N1B4_42 0.42 -0.3 49,726 + Pf1N1B4_42 0.42 -1.3 49,726 + Pf1N1B4_42 0.42 -1.3 49,726 + Pf1N1B4_42 0.42 -0.3 49,727 - Pf1N1B4_42 0.42 +1.3 49,727 - Pf1N1B4_42 0.42 -2.3 49,727 - Pf1N1B4_42 0.42 +0.7 49,727 - Pf1N1B4_42 0.42 +0.7 49,727 - Pf1N1B4_42 0.42 +0.3 49,727 - Pf1N1B4_42 0.42 +0.7 49,727 - Pf1N1B4_42 0.42 -0.3 49,727 - Pf1N1B4_42 0.42 +0.7 49,727 - Pf1N1B4_42 0.42 -0.8 49,727 - Pf1N1B4_42 0.42 -0.7 49,727 - Pf1N1B4_42 0.42 +1.7 49,814 - Pf1N1B4_42 0.47 +0.3 49,829 - Pf1N1B4_42 0.48 -0.3 49,870 + Pf1N1B4_42 0.51 -0.3 49,870 + Pf1N1B4_42 0.51 +0.1 49,984 + Pf1N1B4_42 0.59 +0.3 49,984 + Pf1N1B4_42 0.59 -0.3 49,985 - Pf1N1B4_42 0.59 -0.3 49,999 + Pf1N1B4_42 0.60 +0.3 49,999 + Pf1N1B4_42 0.60 -0.3 50,000 - Pf1N1B4_42 0.60 -0.3 50,000 - -0.3 50,212 + -0.3 50,212 + Pf1N1B4_42 0.74 -0.0 50,213 - Pf1N1B4_42 0.74 -0.3 50,412 - Pf1N1B4_42 0.87 +1.5 50,595 - +0.5 50,710 + -0.3 50,841 + -1.3 50,904 - +1.3 50,959 + -1.3 50,987 + -1.3 50,987 + +0.7 50,987 + +0.2 51,128 - +0.7 51,142 - Pf1N1B4_43 0.30 -0.5 51,318 + Pf1N1B4_43 0.54 -0.3 51,318 + Pf1N1B4_43 0.54 +0.5 51,319 - Pf1N1B4_43 0.55 +0.5 51,319 - Pf1N1B4_43 0.55 -0.3 51,319 - Pf1N1B4_43 0.55 +1.2 51,319 - Pf1N1B4_43 0.55 -0.6 51,319 - Pf1N1B4_43 0.55 +0.5 51,319 - Pf1N1B4_43 0.55 -1.3 51,319 - Pf1N1B4_43 0.55 +0.2 51,319 - Pf1N1B4_43 0.55 +0.8 51,319 - Pf1N1B4_43 0.55 +0.7 51,319 - Pf1N1B4_43 0.55 +0.5 51,417 + Pf1N1B4_43 0.69 -0.3 51,418 - -0.3 51,418 - Pf1N1B4_43 0.69 +0.7 51,543 + Pf1N1B4_43 0.86 +1.3 51,543 + Pf1N1B4_43 0.86 -0.3 51,544 - Pf1N1B4_43 0.87 -0.3 51,579 + +1.1 51,580 - +0.1 51,580 - -0.3 51,580 - +1.7 51,580 - +0.7 51,580 - +0.7 51,580 - +1.3 51,581 + +0.2 51,582 - -0.7 51,582 - +0.3 51,582 - -0.3 51,582 - +0.2
Or see this region's nucleotide sequence