Experiment: Adenine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4016 and Pf1N1B4_4017 overlap by 4 nucleotides Pf1N1B4_4017 and Pf1N1B4_4018 are separated by 467 nucleotides
Pf1N1B4_4016 - Heavy metal sensor histidine kinase, at 4,466,486 to 4,467,889
_4016
Pf1N1B4_4017 - DNA-binding heavy metal response regulator, at 4,467,886 to 4,468,560
_4017
Pf1N1B4_4018 - Heavy metal RND efflux outer membrane protein, CzcC family, at 4,469,028 to 4,470,287
_4018
Position (kb)
4467
4468
4469 Strain fitness (log2 ratio)
-2
-1
0
1 at 4466.979 kb on - strand at 4467.052 kb on - strand at 4467.614 kb on + strand, within Pf1N1B4_4016 at 4467.615 kb on - strand, within Pf1N1B4_4016 at 4467.630 kb on + strand, within Pf1N1B4_4016 at 4467.802 kb on - strand at 4467.845 kb on - strand at 4467.908 kb on + strand at 4468.272 kb on - strand, within Pf1N1B4_4017 at 4468.272 kb on - strand, within Pf1N1B4_4017 at 4468.415 kb on - strand, within Pf1N1B4_4017 at 4468.427 kb on + strand, within Pf1N1B4_4017 at 4468.428 kb on - strand, within Pf1N1B4_4017 at 4468.486 kb on - strand, within Pf1N1B4_4017 at 4468.513 kb on + strand at 4468.514 kb on - strand at 4468.514 kb on - strand at 4468.593 kb on - strand at 4468.823 kb on + strand at 4468.824 kb on - strand at 4468.849 kb on + strand at 4468.854 kb on - strand at 4468.874 kb on + strand at 4468.875 kb on - strand at 4468.875 kb on - strand at 4468.941 kb on + strand at 4469.038 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine (N) remove 4,466,979 - -0.3 4,467,052 - +0.2 4,467,614 + Pf1N1B4_4016 0.80 -1.3 4,467,615 - Pf1N1B4_4016 0.80 -0.3 4,467,630 + Pf1N1B4_4016 0.81 +0.7 4,467,802 - -0.9 4,467,845 - -1.3 4,467,908 + -1.3 4,468,272 - Pf1N1B4_4017 0.57 +0.2 4,468,272 - Pf1N1B4_4017 0.57 +0.7 4,468,415 - Pf1N1B4_4017 0.78 +1.0 4,468,427 + Pf1N1B4_4017 0.80 +0.2 4,468,428 - Pf1N1B4_4017 0.80 +0.9 4,468,486 - Pf1N1B4_4017 0.89 +1.0 4,468,513 + -0.3 4,468,514 - -0.3 4,468,514 - -1.3 4,468,593 - -1.3 4,468,823 + -1.3 4,468,824 - -0.3 4,468,849 + -0.6 4,468,854 - +1.2 4,468,874 + -1.9 4,468,875 - -0.3 4,468,875 - +0.9 4,468,941 + +0.4 4,469,038 + +0.7
Or see this region's nucleotide sequence