Experiment: Adenine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2740 and Pf1N1B4_2741 overlap by 4 nucleotides Pf1N1B4_2741 and Pf1N1B4_2742 are separated by 141 nucleotides Pf1N1B4_2742 and Pf1N1B4_2743 overlap by 1 nucleotides
Pf1N1B4_2740 - Acyl-CoA thioesterase II (EC 3.1.2.-), at 3,078,941 to 3,079,810
_2740
Pf1N1B4_2741 - Hydrolase in polyol utilization gene cluster, haloacid dehalogenase-like family, at 3,079,807 to 3,080,400
_2741
Pf1N1B4_2742 - putative exported protein, at 3,080,542 to 3,081,501
_2742
Pf1N1B4_2743 - YpfJ protein, zinc metalloprotease superfamily, at 3,081,501 to 3,082,394
_2743
Position (kb)
3080
3081
3082 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3079.680 kb on - strand, within Pf1N1B4_2740 at 3080.118 kb on - strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.159 kb on + strand, within Pf1N1B4_2741 at 3080.160 kb on - strand, within Pf1N1B4_2741 at 3080.160 kb on - strand, within Pf1N1B4_2741 at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.405 kb on + strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.406 kb on - strand at 3080.478 kb on + strand at 3080.580 kb on + strand at 3080.581 kb on - strand at 3080.727 kb on + strand, within Pf1N1B4_2742 at 3080.770 kb on + strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.771 kb on - strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.774 kb on + strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.775 kb on - strand, within Pf1N1B4_2742 at 3080.795 kb on + strand, within Pf1N1B4_2742 at 3080.795 kb on + strand, within Pf1N1B4_2742 at 3080.795 kb on + strand, within Pf1N1B4_2742 at 3080.834 kb on + strand, within Pf1N1B4_2742 at 3080.834 kb on + strand, within Pf1N1B4_2742 at 3080.835 kb on - strand, within Pf1N1B4_2742 at 3080.859 kb on - strand, within Pf1N1B4_2742 at 3080.859 kb on - strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.924 kb on + strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.925 kb on - strand, within Pf1N1B4_2742 at 3080.930 kb on + strand, within Pf1N1B4_2742 at 3080.930 kb on + strand, within Pf1N1B4_2742 at 3080.930 kb on + strand, within Pf1N1B4_2742 at 3080.931 kb on - strand, within Pf1N1B4_2742 at 3080.931 kb on - strand, within Pf1N1B4_2742 at 3080.931 kb on - strand, within Pf1N1B4_2742 at 3080.954 kb on + strand, within Pf1N1B4_2742 at 3080.954 kb on + strand, within Pf1N1B4_2742 at 3080.954 kb on + strand, within Pf1N1B4_2742 at 3080.955 kb on - strand, within Pf1N1B4_2742 at 3080.955 kb on - strand, within Pf1N1B4_2742 at 3081.136 kb on - strand, within Pf1N1B4_2742 at 3081.188 kb on - strand, within Pf1N1B4_2742 at 3081.188 kb on - strand, within Pf1N1B4_2742 at 3081.308 kb on + strand, within Pf1N1B4_2742 at 3081.309 kb on - strand at 3081.475 kb on + strand at 3081.495 kb on + strand at 3081.495 kb on + strand at 3081.495 kb on + strand at 3081.495 kb on + strand at 3081.496 kb on - strand at 3081.496 kb on - strand at 3081.496 kb on - strand at 3081.496 kb on - strand at 3081.882 kb on + strand, within Pf1N1B4_2743 at 3081.883 kb on - strand, within Pf1N1B4_2743 at 3081.883 kb on - strand, within Pf1N1B4_2743 at 3081.883 kb on - strand, within Pf1N1B4_2743 at 3081.883 kb on - strand, within Pf1N1B4_2743 at 3081.883 kb on - strand, within Pf1N1B4_2743 at 3081.886 kb on + strand, within Pf1N1B4_2743 at 3081.887 kb on - strand, within Pf1N1B4_2743 at 3081.887 kb on - strand at 3081.887 kb on - strand, within Pf1N1B4_2743 at 3081.955 kb on + strand, within Pf1N1B4_2743 at 3081.955 kb on + strand, within Pf1N1B4_2743 at 3081.955 kb on + strand, within Pf1N1B4_2743 at 3081.955 kb on + strand, within Pf1N1B4_2743 at 3081.955 kb on + strand, within Pf1N1B4_2743 at 3081.956 kb on - strand, within Pf1N1B4_2743 at 3081.956 kb on - strand, within Pf1N1B4_2743 at 3081.956 kb on - strand, within Pf1N1B4_2743 at 3081.979 kb on + strand, within Pf1N1B4_2743 at 3081.979 kb on + strand, within Pf1N1B4_2743 at 3081.979 kb on + strand, within Pf1N1B4_2743 at 3081.979 kb on + strand, within Pf1N1B4_2743 at 3081.979 kb on + strand, within Pf1N1B4_2743 at 3081.979 kb on + strand, within Pf1N1B4_2743 at 3081.980 kb on - strand, within Pf1N1B4_2743 at 3082.058 kb on + strand, within Pf1N1B4_2743 at 3082.058 kb on + strand, within Pf1N1B4_2743 at 3082.058 kb on + strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.059 kb on - strand, within Pf1N1B4_2743 at 3082.061 kb on - strand, within Pf1N1B4_2743 at 3082.061 kb on - strand, within Pf1N1B4_2743 at 3082.451 kb on + strand at 3082.451 kb on + strand at 3082.452 kb on - strand at 3082.452 kb on - strand at 3082.452 kb on - strand at 3082.452 kb on - strand at 3082.452 kb on - strand at 3082.452 kb on - strand at 3082.452 kb on - strand at 3082.452 kb on - strand at 3082.455 kb on + strand at 3082.456 kb on - strand at 3082.456 kb on - strand at 3082.456 kb on - strand at 3082.456 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine (N) remove 3,079,680 - Pf1N1B4_2740 0.85 +0.2 3,080,118 - Pf1N1B4_2741 0.52 -0.8 3,080,159 + Pf1N1B4_2741 0.59 -1.8 3,080,159 + Pf1N1B4_2741 0.59 +0.2 3,080,159 + Pf1N1B4_2741 0.59 +0.5 3,080,159 + Pf1N1B4_2741 0.59 -0.5 3,080,160 - Pf1N1B4_2741 0.59 -0.2 3,080,160 - Pf1N1B4_2741 0.59 -1.1 3,080,405 + +1.2 3,080,405 + -2.2 3,080,405 + -0.4 3,080,405 + -0.4 3,080,405 + +1.4 3,080,405 + +0.2 3,080,405 + +0.8 3,080,405 + +0.2 3,080,405 + -0.3 3,080,406 - +0.4 3,080,406 - -1.2 3,080,406 - +0.8 3,080,406 - +1.8 3,080,406 - +1.2 3,080,406 - +1.5 3,080,478 + +0.2 3,080,580 + +2.2 3,080,581 - +0.8 3,080,727 + Pf1N1B4_2742 0.19 -0.2 3,080,770 + Pf1N1B4_2742 0.24 -0.6 3,080,771 - Pf1N1B4_2742 0.24 -1.1 3,080,771 - Pf1N1B4_2742 0.24 +0.2 3,080,771 - Pf1N1B4_2742 0.24 -0.8 3,080,771 - Pf1N1B4_2742 0.24 +0.2 3,080,774 + Pf1N1B4_2742 0.24 +1.2 3,080,774 + Pf1N1B4_2742 0.24 -1.4 3,080,774 + Pf1N1B4_2742 0.24 +2.0 3,080,774 + Pf1N1B4_2742 0.24 -1.2 3,080,775 - Pf1N1B4_2742 0.24 -1.4 3,080,775 - Pf1N1B4_2742 0.24 -0.8 3,080,775 - Pf1N1B4_2742 0.24 +0.2 3,080,775 - Pf1N1B4_2742 0.24 +0.6 3,080,795 + Pf1N1B4_2742 0.26 -0.1 3,080,795 + Pf1N1B4_2742 0.26 +0.8 3,080,795 + Pf1N1B4_2742 0.26 +1.2 3,080,834 + Pf1N1B4_2742 0.30 +0.2 3,080,834 + Pf1N1B4_2742 0.30 +0.5 3,080,835 - Pf1N1B4_2742 0.31 +1.8 3,080,859 - Pf1N1B4_2742 0.33 +0.2 3,080,859 - Pf1N1B4_2742 0.33 +0.2 3,080,924 + Pf1N1B4_2742 0.40 +0.2 3,080,924 + Pf1N1B4_2742 0.40 +0.2 3,080,924 + Pf1N1B4_2742 0.40 +1.8 3,080,924 + Pf1N1B4_2742 0.40 +2.0 3,080,924 + Pf1N1B4_2742 0.40 +0.4 3,080,924 + Pf1N1B4_2742 0.40 -1.2 3,080,924 + Pf1N1B4_2742 0.40 +0.8 3,080,925 - Pf1N1B4_2742 0.40 +1.2 3,080,925 - Pf1N1B4_2742 0.40 +1.6 3,080,925 - Pf1N1B4_2742 0.40 +1.4 3,080,925 - Pf1N1B4_2742 0.40 -2.2 3,080,925 - Pf1N1B4_2742 0.40 +0.5 3,080,930 + Pf1N1B4_2742 0.40 -0.8 3,080,930 + Pf1N1B4_2742 0.40 +0.2 3,080,930 + Pf1N1B4_2742 0.40 -0.1 3,080,931 - Pf1N1B4_2742 0.41 +0.8 3,080,931 - Pf1N1B4_2742 0.41 +1.2 3,080,931 - Pf1N1B4_2742 0.41 +1.2 3,080,954 + Pf1N1B4_2742 0.43 +0.6 3,080,954 + Pf1N1B4_2742 0.43 +0.5 3,080,954 + Pf1N1B4_2742 0.43 +0.4 3,080,955 - Pf1N1B4_2742 0.43 +0.2 3,080,955 - Pf1N1B4_2742 0.43 +0.8 3,081,136 - Pf1N1B4_2742 0.62 +0.8 3,081,188 - Pf1N1B4_2742 0.67 +0.2 3,081,188 - Pf1N1B4_2742 0.67 +3.3 3,081,308 + Pf1N1B4_2742 0.80 +0.8 3,081,309 - -0.8 3,081,475 + +1.2 3,081,495 + -0.4 3,081,495 + -0.8 3,081,495 + +0.6 3,081,495 + -1.4 3,081,496 - +1.2 3,081,496 - +0.2 3,081,496 - -0.2 3,081,496 - +1.2 3,081,882 + Pf1N1B4_2743 0.43 +1.5 3,081,883 - Pf1N1B4_2743 0.43 +0.2 3,081,883 - Pf1N1B4_2743 0.43 +2.0 3,081,883 - Pf1N1B4_2743 0.43 -0.8 3,081,883 - Pf1N1B4_2743 0.43 +1.7 3,081,883 - Pf1N1B4_2743 0.43 -0.8 3,081,886 + Pf1N1B4_2743 0.43 +0.9 3,081,887 - Pf1N1B4_2743 0.43 -0.4 3,081,887 - +0.2 3,081,887 - Pf1N1B4_2743 0.43 -0.4 3,081,955 + Pf1N1B4_2743 0.51 -0.8 3,081,955 + Pf1N1B4_2743 0.51 +0.7 3,081,955 + Pf1N1B4_2743 0.51 +0.2 3,081,955 + Pf1N1B4_2743 0.51 +0.2 3,081,955 + Pf1N1B4_2743 0.51 +2.2 3,081,956 - Pf1N1B4_2743 0.51 -0.7 3,081,956 - Pf1N1B4_2743 0.51 +0.7 3,081,956 - Pf1N1B4_2743 0.51 +0.2 3,081,979 + Pf1N1B4_2743 0.53 +0.6 3,081,979 + Pf1N1B4_2743 0.53 +0.7 3,081,979 + Pf1N1B4_2743 0.53 +1.7 3,081,979 + Pf1N1B4_2743 0.53 +1.2 3,081,979 + Pf1N1B4_2743 0.53 +0.2 3,081,979 + Pf1N1B4_2743 0.53 +0.2 3,081,980 - Pf1N1B4_2743 0.54 +0.2 3,082,058 + Pf1N1B4_2743 0.62 +0.2 3,082,058 + Pf1N1B4_2743 0.62 +2.7 3,082,058 + Pf1N1B4_2743 0.62 +0.2 3,082,059 - Pf1N1B4_2743 0.62 -0.4 3,082,059 - Pf1N1B4_2743 0.62 +2.2 3,082,059 - Pf1N1B4_2743 0.62 +0.8 3,082,059 - Pf1N1B4_2743 0.62 +0.2 3,082,059 - Pf1N1B4_2743 0.62 -2.2 3,082,059 - Pf1N1B4_2743 0.62 +3.2 3,082,059 - Pf1N1B4_2743 0.62 +0.6 3,082,059 - Pf1N1B4_2743 0.62 +0.4 3,082,059 - Pf1N1B4_2743 0.62 +2.2 3,082,059 - Pf1N1B4_2743 0.62 +1.2 3,082,059 - Pf1N1B4_2743 0.62 -0.4 3,082,059 - Pf1N1B4_2743 0.62 +0.2 3,082,061 - Pf1N1B4_2743 0.63 +0.5 3,082,061 - Pf1N1B4_2743 0.63 +2.2 3,082,451 + +0.2 3,082,451 + +0.7 3,082,452 - +0.2 3,082,452 - +0.0 3,082,452 - -1.8 3,082,452 - +1.2 3,082,452 - +2.5 3,082,452 - -0.6 3,082,452 - +2.5 3,082,452 - +0.2 3,082,455 + -1.4 3,082,456 - +0.7 3,082,456 - +1.0 3,082,456 - +2.2 3,082,456 - -0.4
Or see this region's nucleotide sequence