Experiment: Adenine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1016 and Pf1N1B4_1017 are separated by 359 nucleotides Pf1N1B4_1017 and Pf1N1B4_1018 are separated by 66 nucleotides Pf1N1B4_1018 and Pf1N1B4_1019 are separated by 28 nucleotides
Pf1N1B4_1016 - FIG00954263: hypothetical protein, at 1,151,995 to 1,152,495
_1016
Pf1N1B4_1017 - FIG00957554: hypothetical protein, at 1,152,855 to 1,153,220
_1017
Pf1N1B4_1018 - Dihydrolipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex (EC 2.3.1.168), at 1,153,287 to 1,154,396
_1018
Pf1N1B4_1019 - Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4), at 1,154,425 to 1,155,414
_1019
Position (kb)
1153
1154
1155 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1152.293 kb on + strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.294 kb on - strand, within Pf1N1B4_1016 at 1152.407 kb on + strand, within Pf1N1B4_1016 at 1152.408 kb on - strand, within Pf1N1B4_1016 at 1152.746 kb on + strand at 1152.746 kb on + strand at 1152.747 kb on - strand at 1152.747 kb on - strand at 1152.747 kb on - strand at 1152.747 kb on - strand at 1152.751 kb on + strand at 1152.751 kb on + strand at 1152.752 kb on - strand at 1152.752 kb on - strand at 1152.752 kb on - strand at 1152.752 kb on - strand at 1152.752 kb on - strand at 1152.785 kb on + strand at 1152.800 kb on - strand at 1152.800 kb on - strand at 1152.804 kb on - strand at 1152.812 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.829 kb on + strand at 1152.830 kb on - strand at 1152.830 kb on - strand at 1152.830 kb on - strand at 1152.830 kb on - strand at 1152.830 kb on - strand at 1152.871 kb on + strand at 1152.871 kb on + strand at 1152.871 kb on + strand at 1152.872 kb on - strand at 1152.872 kb on - strand at 1152.872 kb on - strand at 1152.872 kb on - strand at 1152.872 kb on - strand at 1152.873 kb on + strand at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand, within Pf1N1B4_1017 at 1153.026 kb on + strand at 1153.026 kb on + strand at 1153.027 kb on - strand, within Pf1N1B4_1017 at 1153.048 kb on + strand, within Pf1N1B4_1017 at 1153.048 kb on + strand, within Pf1N1B4_1017 at 1153.048 kb on + strand, within Pf1N1B4_1017 at 1153.048 kb on + strand at 1153.048 kb on + strand, within Pf1N1B4_1017 at 1153.049 kb on - strand, within Pf1N1B4_1017 at 1153.049 kb on - strand, within Pf1N1B4_1017 at 1153.049 kb on - strand, within Pf1N1B4_1017 at 1153.150 kb on + strand, within Pf1N1B4_1017 at 1153.150 kb on + strand, within Pf1N1B4_1017 at 1153.150 kb on + strand, within Pf1N1B4_1017 at 1153.150 kb on + strand at 1153.218 kb on + strand at 1153.218 kb on + strand at 1153.218 kb on + strand at 1153.218 kb on + strand at 1153.218 kb on + strand at 1153.219 kb on - strand at 1153.219 kb on - strand at 1153.238 kb on + strand at 1153.238 kb on + strand at 1153.238 kb on + strand at 1153.239 kb on - strand at 1153.288 kb on + strand at 1153.288 kb on + strand at 1153.289 kb on - strand at 1153.289 kb on - strand at 1153.753 kb on - strand, within Pf1N1B4_1018 at 1153.900 kb on - strand, within Pf1N1B4_1018 at 1153.900 kb on - strand, within Pf1N1B4_1018 at 1153.900 kb on - strand, within Pf1N1B4_1018 at 1153.900 kb on - strand, within Pf1N1B4_1018 at 1154.173 kb on + strand, within Pf1N1B4_1018 at 1154.245 kb on + strand, within Pf1N1B4_1018 at 1154.389 kb on + strand at 1154.389 kb on + strand at 1154.390 kb on - strand at 1154.390 kb on - strand at 1154.390 kb on - strand at 1154.432 kb on + strand at 1154.433 kb on - strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.486 kb on + strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.487 kb on - strand at 1154.504 kb on + strand at 1154.577 kb on - strand, within Pf1N1B4_1019 at 1154.793 kb on + strand, within Pf1N1B4_1019 at 1154.793 kb on + strand, within Pf1N1B4_1019 at 1154.793 kb on + strand, within Pf1N1B4_1019 at 1154.794 kb on - strand, within Pf1N1B4_1019 at 1154.794 kb on - strand, within Pf1N1B4_1019 at 1154.794 kb on - strand, within Pf1N1B4_1019 at 1154.880 kb on - strand, within Pf1N1B4_1019 at 1154.902 kb on + strand, within Pf1N1B4_1019 at 1154.902 kb on + strand, within Pf1N1B4_1019 at 1154.903 kb on - strand, within Pf1N1B4_1019 at 1154.903 kb on - strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.146 kb on + strand, within Pf1N1B4_1019 at 1155.147 kb on - strand, within Pf1N1B4_1019 at 1155.147 kb on - strand, within Pf1N1B4_1019 at 1155.147 kb on - strand at 1155.147 kb on - strand, within Pf1N1B4_1019 at 1155.311 kb on - strand, within Pf1N1B4_1019
Per-strain Table
Position Strand Gene LocusTag Fraction Adenine (N) remove 1,152,293 + Pf1N1B4_1016 0.59 +1.3 1,152,294 - Pf1N1B4_1016 0.60 -0.6 1,152,294 - Pf1N1B4_1016 0.60 -2.4 1,152,294 - Pf1N1B4_1016 0.60 +1.1 1,152,294 - Pf1N1B4_1016 0.60 +1.1 1,152,294 - Pf1N1B4_1016 0.60 +1.5 1,152,294 - Pf1N1B4_1016 0.60 -0.9 1,152,407 + Pf1N1B4_1016 0.82 +0.6 1,152,408 - Pf1N1B4_1016 0.82 -0.9 1,152,746 + +0.1 1,152,746 + +1.1 1,152,747 - +0.1 1,152,747 - +1.1 1,152,747 - +1.4 1,152,747 - +0.7 1,152,751 + -1.4 1,152,751 + +1.1 1,152,752 - +0.4 1,152,752 - +0.9 1,152,752 - +0.7 1,152,752 - +0.1 1,152,752 - +1.2 1,152,785 + +0.1 1,152,800 - -0.9 1,152,800 - +1.5 1,152,804 - +2.5 1,152,812 + -1.9 1,152,829 + -0.3 1,152,829 + +0.7 1,152,829 + -1.9 1,152,829 + -0.2 1,152,829 + -1.4 1,152,829 + -0.9 1,152,829 + +0.4 1,152,830 - -0.6 1,152,830 - +1.1 1,152,830 - +0.6 1,152,830 - -1.2 1,152,830 - +0.7 1,152,871 + +0.1 1,152,871 + -0.6 1,152,871 + -0.4 1,152,872 - +1.1 1,152,872 - -0.9 1,152,872 - +1.1 1,152,872 - +1.7 1,152,872 - +1.2 1,152,873 + +1.1 1,153,026 + Pf1N1B4_1017 0.47 -0.9 1,153,026 + Pf1N1B4_1017 0.47 -0.9 1,153,026 + Pf1N1B4_1017 0.47 +2.7 1,153,026 + Pf1N1B4_1017 0.47 +0.1 1,153,026 + Pf1N1B4_1017 0.47 +1.1 1,153,026 + Pf1N1B4_1017 0.47 +0.6 1,153,026 + -1.9 1,153,026 + -0.9 1,153,027 - Pf1N1B4_1017 0.47 +0.7 1,153,048 + Pf1N1B4_1017 0.53 +1.5 1,153,048 + Pf1N1B4_1017 0.53 +1.1 1,153,048 + Pf1N1B4_1017 0.53 +0.1 1,153,048 + +0.1 1,153,048 + Pf1N1B4_1017 0.53 -0.6 1,153,049 - Pf1N1B4_1017 0.53 +1.7 1,153,049 - Pf1N1B4_1017 0.53 -1.1 1,153,049 - Pf1N1B4_1017 0.53 +2.7 1,153,150 + Pf1N1B4_1017 0.81 +1.1 1,153,150 + Pf1N1B4_1017 0.81 +2.1 1,153,150 + Pf1N1B4_1017 0.81 -1.1 1,153,150 + -0.4 1,153,218 + +0.4 1,153,218 + -0.2 1,153,218 + +0.1 1,153,218 + +1.7 1,153,218 + +0.1 1,153,219 - +1.1 1,153,219 - -0.1 1,153,238 + -0.4 1,153,238 + -0.9 1,153,238 + +0.1 1,153,239 - +1.7 1,153,288 + -0.9 1,153,288 + -0.9 1,153,289 - +0.7 1,153,289 - +0.1 1,153,753 - Pf1N1B4_1018 0.42 +1.2 1,153,900 - Pf1N1B4_1018 0.55 -1.4 1,153,900 - Pf1N1B4_1018 0.55 +1.1 1,153,900 - Pf1N1B4_1018 0.55 +0.7 1,153,900 - Pf1N1B4_1018 0.55 +0.6 1,154,173 + Pf1N1B4_1018 0.80 +1.3 1,154,245 + Pf1N1B4_1018 0.86 +1.1 1,154,389 + -0.4 1,154,389 + +0.1 1,154,390 - +0.1 1,154,390 - +1.7 1,154,390 - -0.9 1,154,432 + +0.5 1,154,433 - -1.9 1,154,486 + +1.5 1,154,486 + +1.1 1,154,486 + +1.7 1,154,486 + +1.1 1,154,486 + -0.6 1,154,486 + -1.2 1,154,486 + +2.0 1,154,486 + +0.1 1,154,486 + -1.4 1,154,486 + -1.6 1,154,486 + +1.7 1,154,486 + +0.3 1,154,487 - +0.3 1,154,487 - +0.6 1,154,487 - +1.5 1,154,487 - -0.9 1,154,487 - +0.7 1,154,504 + +1.8 1,154,577 - Pf1N1B4_1019 0.15 -0.9 1,154,793 + Pf1N1B4_1019 0.37 -0.9 1,154,793 + Pf1N1B4_1019 0.37 +2.5 1,154,793 + Pf1N1B4_1019 0.37 +0.1 1,154,794 - Pf1N1B4_1019 0.37 +0.1 1,154,794 - Pf1N1B4_1019 0.37 -0.3 1,154,794 - Pf1N1B4_1019 0.37 +0.6 1,154,880 - Pf1N1B4_1019 0.46 +0.1 1,154,902 + Pf1N1B4_1019 0.48 +0.1 1,154,902 + Pf1N1B4_1019 0.48 +1.1 1,154,903 - Pf1N1B4_1019 0.48 +1.1 1,154,903 - Pf1N1B4_1019 0.48 -0.9 1,155,146 + Pf1N1B4_1019 0.73 -0.4 1,155,146 + Pf1N1B4_1019 0.73 +0.7 1,155,146 + Pf1N1B4_1019 0.73 +0.1 1,155,146 + Pf1N1B4_1019 0.73 -0.9 1,155,147 - Pf1N1B4_1019 0.73 -0.9 1,155,147 - Pf1N1B4_1019 0.73 +1.1 1,155,147 - +0.1 1,155,147 - Pf1N1B4_1019 0.73 -0.1 1,155,311 - Pf1N1B4_1019 0.89 +1.5
Or see this region's nucleotide sequence