Experiment: ALP with Cobalt chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3430 and Dsui_3431 overlap by 4 nucleotides Dsui_3431 and Dsui_3432 overlap by 1 nucleotides Dsui_3432 and Dsui_3433 are separated by 124 nucleotides Dsui_3433 and Dsui_3434 are separated by 82 nucleotides
Dsui_3430: Dsui_3430 - heavy metal efflux pump, cobalt-zinc-cadmium, at 3,681,043 to 3,684,129
_3430
Dsui_3431: Dsui_3431 - Protein of unknown function (DUF3240), at 3,684,126 to 3,684,446
_3431
Dsui_3432: Dsui_3432 - outer membrane protein, at 3,684,446 to 3,685,723
_3432
Dsui_3433: Dsui_3433 - putative acetyltransferase, at 3,685,848 to 3,686,354
_3433
Dsui_3434: Dsui_3434 - hypothetical protein, at 3,686,437 to 3,686,694
_3434
Position (kb)
3684
3685
3686 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3683.504 kb on + strand, within Dsui_3430 at 3683.538 kb on + strand, within Dsui_3430 at 3683.546 kb on - strand, within Dsui_3430 at 3683.653 kb on - strand at 3683.716 kb on - strand, within Dsui_3430 at 3683.796 kb on + strand, within Dsui_3430 at 3683.979 kb on - strand at 3684.047 kb on + strand at 3684.047 kb on + strand at 3684.055 kb on + strand at 3684.110 kb on - strand at 3684.130 kb on + strand at 3684.130 kb on - strand at 3684.131 kb on - strand at 3684.210 kb on - strand, within Dsui_3431 at 3684.226 kb on - strand, within Dsui_3431 at 3684.354 kb on + strand, within Dsui_3431 at 3684.363 kb on + strand, within Dsui_3431 at 3684.407 kb on + strand, within Dsui_3431 at 3684.420 kb on - strand at 3684.521 kb on + strand at 3684.589 kb on + strand, within Dsui_3432 at 3684.597 kb on - strand, within Dsui_3432 at 3684.648 kb on - strand at 3684.702 kb on - strand, within Dsui_3432 at 3684.955 kb on + strand, within Dsui_3432 at 3685.030 kb on + strand, within Dsui_3432 at 3685.038 kb on - strand at 3685.049 kb on - strand at 3685.194 kb on + strand, within Dsui_3432 at 3685.212 kb on - strand at 3685.246 kb on + strand, within Dsui_3432 at 3685.251 kb on + strand, within Dsui_3432 at 3685.275 kb on + strand, within Dsui_3432 at 3685.283 kb on - strand at 3685.283 kb on - strand, within Dsui_3432 at 3685.396 kb on - strand, within Dsui_3432 at 3685.435 kb on + strand, within Dsui_3432 at 3685.680 kb on + strand at 3685.688 kb on - strand at 3685.763 kb on + strand at 3686.050 kb on - strand, within Dsui_3433 at 3686.124 kb on - strand, within Dsui_3433 at 3686.217 kb on - strand at 3686.226 kb on + strand at 3686.288 kb on - strand at 3686.294 kb on - strand at 3686.389 kb on - strand at 3686.404 kb on - strand at 3686.467 kb on + strand, within Dsui_3434 at 3686.528 kb on + strand, within Dsui_3434 at 3686.678 kb on + strand at 3686.698 kb on + strand at 3686.723 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cobalt chloride 0.25 mM remove 3,683,504 + Dsui_3430 0.80 -5.4 3,683,538 + Dsui_3430 0.81 -4.2 3,683,546 - Dsui_3430 0.81 -1.6 3,683,653 - +0.8 3,683,716 - Dsui_3430 0.87 -0.8 3,683,796 + Dsui_3430 0.89 -4.1 3,683,979 - +0.8 3,684,047 + -3.9 3,684,047 + -3.8 3,684,055 + -5.6 3,684,110 - +0.8 3,684,130 + -1.8 3,684,130 - +0.8 3,684,131 - +0.8 3,684,210 - Dsui_3431 0.26 -0.2 3,684,226 - Dsui_3431 0.31 -1.8 3,684,354 + Dsui_3431 0.71 -0.8 3,684,363 + Dsui_3431 0.74 -0.2 3,684,407 + Dsui_3431 0.88 -0.2 3,684,420 - +0.8 3,684,521 + -4.1 3,684,589 + Dsui_3432 0.11 -3.3 3,684,597 - Dsui_3432 0.12 +0.8 3,684,648 - -0.2 3,684,702 - Dsui_3432 0.20 -2.0 3,684,955 + Dsui_3432 0.40 -5.2 3,685,030 + Dsui_3432 0.46 -4.2 3,685,038 - +0.8 3,685,049 - -0.2 3,685,194 + Dsui_3432 0.59 -4.5 3,685,212 - +0.8 3,685,246 + Dsui_3432 0.63 -5.7 3,685,251 + Dsui_3432 0.63 -4.0 3,685,275 + Dsui_3432 0.65 -4.9 3,685,283 - +0.8 3,685,283 - Dsui_3432 0.65 +0.8 3,685,396 - Dsui_3432 0.74 -1.6 3,685,435 + Dsui_3432 0.77 -5.0 3,685,680 + -2.0 3,685,688 - -1.2 3,685,763 + -0.2 3,686,050 - Dsui_3433 0.40 -1.8 3,686,124 - Dsui_3433 0.54 -0.2 3,686,217 - -0.2 3,686,226 + +0.8 3,686,288 - +0.8 3,686,294 - +0.8 3,686,389 - -0.2 3,686,404 - +0.8 3,686,467 + Dsui_3434 0.12 +0.8 3,686,528 + Dsui_3434 0.35 -2.0 3,686,678 + -0.2 3,686,698 + +0.8 3,686,723 + +0.0
Or see this region's nucleotide sequence