Experiment: ALP with Cobalt chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_2723 and Dsui_2724 are separated by 36 nucleotides Dsui_2724 and Dsui_2725 are separated by 191 nucleotides Dsui_2725 and Dsui_2726 overlap by 11 nucleotides Dsui_2726 and Dsui_2727 overlap by 20 nucleotides
Dsui_2723: Dsui_2723 - transcription termination factor NusA, at 2,878,955 to 2,880,427
_2723
Dsui_2724: Dsui_2724 - hypothetical protein, at 2,880,464 to 2,880,925
_2724
Dsui_2725: Dsui_2725 - pseudouridine synthase family protein, at 2,881,117 to 2,882,262
_2725
Dsui_2726: Dsui_2726 - segregation and condensation protein B, at 2,882,252 to 2,882,809
_2726
Dsui_2727: Dsui_2727 - hypothetical protein, at 2,882,790 to 2,883,713
_2727
Position (kb)
2881
2882
2883 Strain fitness (log2 ratio)
-1
0
1 at 2880.457 kb on - strand at 2880.491 kb on - strand at 2880.922 kb on - strand at 2880.922 kb on - strand at 2881.054 kb on + strand at 2881.157 kb on + strand at 2882.283 kb on - strand at 2882.331 kb on - strand, within Dsui_2726 at 2882.495 kb on - strand, within Dsui_2726 at 2882.870 kb on - strand at 2882.900 kb on - strand, within Dsui_2727 at 2882.958 kb on - strand, within Dsui_2727 at 2882.968 kb on - strand, within Dsui_2727 at 2883.073 kb on - strand, within Dsui_2727 at 2883.228 kb on - strand, within Dsui_2727
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cobalt chloride 0.25 mM remove 2,880,457 - -0.5 2,880,491 - -1.3 2,880,922 - -0.1 2,880,922 - -1.5 2,881,054 + +0.7 2,881,157 + -1.4 2,882,283 - -0.4 2,882,331 - Dsui_2726 0.14 -1.1 2,882,495 - Dsui_2726 0.44 -1.7 2,882,870 - -1.0 2,882,900 - Dsui_2727 0.12 -0.7 2,882,958 - Dsui_2727 0.18 -1.2 2,882,968 - Dsui_2727 0.19 -1.1 2,883,073 - Dsui_2727 0.31 -0.4 2,883,228 - Dsui_2727 0.47 +0.5
Or see this region's nucleotide sequence