Experiment: ALP with Cobalt chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1824 and Dsui_1825 are separated by 455 nucleotides Dsui_1825 and Dsui_1826 are separated by 72 nucleotides Dsui_1826 and Dsui_1827 are separated by 211 nucleotides Dsui_1827 and Dsui_1828 are separated by 24 nucleotides
Dsui_1824: Dsui_1824 - PAS domain S-box/diguanylate cyclase (GGDEF) domain-containing protein, at 1,921,737 to 1,923,686
_1824
Dsui_1825: Dsui_1825 - NAD(P)H-dependent nitrite reductase, large subunit, at 1,924,142 to 1,926,580
_1825
Dsui_1826: Dsui_1826 - NAD(P)H-dependent nitrite reductase, small subunit, at 1,926,653 to 1,926,961
_1826
Dsui_1827: Dsui_1827 - hypothetical protein, at 1,927,173 to 1,927,541
_1827
Dsui_1828: Dsui_1828 - response regulator with putative antiterminator output domain, at 1,927,566 to 1,928,141
_1828
Position (kb)
1924
1925
1926
1927 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1923.142 kb on + strand at 1923.142 kb on + strand at 1923.160 kb on - strand at 1923.272 kb on - strand at 1923.301 kb on - strand, within Dsui_1824 at 1923.375 kb on + strand at 1923.378 kb on - strand, within Dsui_1824 at 1923.543 kb on - strand at 1923.756 kb on + strand at 1923.937 kb on + strand at 1923.964 kb on - strand at 1924.000 kb on - strand at 1924.073 kb on + strand at 1924.194 kb on + strand at 1924.194 kb on + strand at 1924.202 kb on - strand at 1924.217 kb on + strand at 1924.629 kb on + strand, within Dsui_1825 at 1924.648 kb on + strand, within Dsui_1825 at 1924.671 kb on + strand, within Dsui_1825 at 1924.692 kb on + strand, within Dsui_1825 at 1924.866 kb on + strand at 1924.873 kb on + strand at 1925.032 kb on + strand, within Dsui_1825 at 1925.032 kb on + strand at 1925.032 kb on + strand at 1925.032 kb on + strand at 1925.073 kb on - strand at 1925.094 kb on + strand, within Dsui_1825 at 1925.112 kb on - strand at 1925.241 kb on - strand at 1925.438 kb on - strand, within Dsui_1825 at 1925.451 kb on - strand, within Dsui_1825 at 1925.477 kb on - strand, within Dsui_1825 at 1925.483 kb on - strand at 1925.505 kb on - strand at 1925.559 kb on - strand at 1925.633 kb on - strand at 1925.634 kb on - strand at 1925.675 kb on - strand at 1925.690 kb on + strand at 1925.735 kb on + strand at 1925.919 kb on + strand, within Dsui_1825 at 1925.919 kb on + strand at 1925.927 kb on - strand, within Dsui_1825 at 1925.982 kb on - strand at 1926.014 kb on + strand at 1926.040 kb on + strand at 1926.171 kb on + strand at 1926.195 kb on - strand, within Dsui_1825 at 1926.310 kb on - strand at 1926.310 kb on - strand at 1926.367 kb on + strand at 1926.406 kb on + strand at 1926.438 kb on - strand at 1926.444 kb on + strand at 1926.477 kb on - strand at 1926.611 kb on + strand at 1926.721 kb on - strand at 1926.737 kb on + strand at 1926.845 kb on + strand at 1926.899 kb on - strand, within Dsui_1826 at 1926.909 kb on - strand, within Dsui_1826 at 1926.950 kb on + strand at 1926.957 kb on + strand at 1927.069 kb on + strand at 1927.086 kb on + strand at 1927.146 kb on + strand at 1927.165 kb on + strand at 1927.167 kb on - strand at 1927.257 kb on + strand, within Dsui_1827 at 1927.382 kb on + strand, within Dsui_1827 at 1927.427 kb on + strand, within Dsui_1827 at 1927.556 kb on + strand at 1927.564 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cobalt chloride 0.25 mM remove 1,923,142 + +0.8 1,923,142 + -0.2 1,923,160 - +0.8 1,923,272 - +0.8 1,923,301 - Dsui_1824 0.80 -0.2 1,923,375 + -0.2 1,923,378 - Dsui_1824 0.84 -1.2 1,923,543 - +0.8 1,923,756 + +0.8 1,923,937 + +0.8 1,923,964 - -1.5 1,924,000 - -0.7 1,924,073 + +0.8 1,924,194 + -0.2 1,924,194 + +0.8 1,924,202 - -0.8 1,924,217 + -0.2 1,924,629 + Dsui_1825 0.20 -2.4 1,924,648 + Dsui_1825 0.21 +0.8 1,924,671 + Dsui_1825 0.22 -2.6 1,924,692 + Dsui_1825 0.23 -0.2 1,924,866 + -0.2 1,924,873 + +0.8 1,925,032 + Dsui_1825 0.36 -0.8 1,925,032 + +0.8 1,925,032 + -0.2 1,925,032 + +0.8 1,925,073 - +0.8 1,925,094 + Dsui_1825 0.39 -2.0 1,925,112 - +0.8 1,925,241 - -1.2 1,925,438 - Dsui_1825 0.53 -1.2 1,925,451 - Dsui_1825 0.54 +0.8 1,925,477 - Dsui_1825 0.55 +0.8 1,925,483 - -0.8 1,925,505 - +0.8 1,925,559 - +0.8 1,925,633 - -0.2 1,925,634 - +0.8 1,925,675 - +0.8 1,925,690 + +0.8 1,925,735 + +0.8 1,925,919 + Dsui_1825 0.73 -0.2 1,925,919 + -0.2 1,925,927 - Dsui_1825 0.73 +0.8 1,925,982 - +0.8 1,926,014 + +0.8 1,926,040 + +0.8 1,926,171 + +0.8 1,926,195 - Dsui_1825 0.84 -1.5 1,926,310 - -0.2 1,926,310 - +0.8 1,926,367 + -1.8 1,926,406 + -0.2 1,926,438 - -0.2 1,926,444 + -0.8 1,926,477 - -0.2 1,926,611 + +0.8 1,926,721 - -0.2 1,926,737 + +0.8 1,926,845 + -0.8 1,926,899 - Dsui_1826 0.80 +0.8 1,926,909 - Dsui_1826 0.83 -1.2 1,926,950 + +0.8 1,926,957 + -1.9 1,927,069 + +0.2 1,927,086 + +0.1 1,927,146 + -0.2 1,927,165 + -0.8 1,927,167 - -0.8 1,927,257 + Dsui_1827 0.23 -0.8 1,927,382 + Dsui_1827 0.57 -3.5 1,927,427 + Dsui_1827 0.69 -1.4 1,927,556 + +0.1 1,927,564 - -2.2
Or see this region's nucleotide sequence