Experiment: ALP with Cobalt chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1809 and Dsui_1810 are separated by 15 nucleotides Dsui_1810 and Dsui_1811 overlap by 1 nucleotides Dsui_1811 and Dsui_1812 are separated by 209 nucleotides Dsui_1812 and Dsui_1813 are separated by 3 nucleotides
Dsui_1809: Dsui_1809 - DNA polymerase III, subunit gamma/tau, at 1,906,920 to 1,908,566
_1809
Dsui_1810: Dsui_1810 - DNA-binding protein, YbaB/EbfC family, at 1,908,582 to 1,908,905
_1810
Dsui_1811: Dsui_1811 - recombination protein RecR, at 1,908,905 to 1,909,507
_1811
Dsui_1812: Dsui_1812 - ubiquinol-cytochrome c reductase, iron-sulfur subunit, at 1,909,717 to 1,910,313
_1812
Dsui_1813: Dsui_1813 - cytochrome b subunit of the bc complex, at 1,910,317 to 1,911,621
_1813
Position (kb)
1908
1909
1910 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1908.608 kb on + strand at 1908.645 kb on - strand at 1908.647 kb on + strand, within Dsui_1810 at 1908.657 kb on + strand, within Dsui_1810 at 1908.657 kb on + strand, within Dsui_1810 at 1908.683 kb on - strand, within Dsui_1810 at 1908.841 kb on - strand, within Dsui_1810 at 1908.868 kb on + strand, within Dsui_1810 at 1908.868 kb on + strand, within Dsui_1810 at 1908.876 kb on - strand at 1908.904 kb on - strand at 1908.934 kb on + strand at 1908.942 kb on - strand at 1908.982 kb on + strand, within Dsui_1811 at 1909.089 kb on - strand, within Dsui_1811 at 1909.097 kb on - strand, within Dsui_1811 at 1909.097 kb on - strand, within Dsui_1811 at 1909.192 kb on + strand, within Dsui_1811 at 1909.195 kb on - strand, within Dsui_1811 at 1909.223 kb on + strand, within Dsui_1811 at 1909.474 kb on + strand at 1909.476 kb on - strand at 1909.539 kb on + strand at 1909.541 kb on - strand at 1909.611 kb on + strand at 1909.662 kb on + strand at 1910.296 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cobalt chloride 0.25 mM remove 1,908,608 + -1.1 1,908,645 - -0.2 1,908,647 + Dsui_1810 0.20 -1.0 1,908,657 + Dsui_1810 0.23 -3.9 1,908,657 + Dsui_1810 0.23 -2.1 1,908,683 - Dsui_1810 0.31 -0.8 1,908,841 - Dsui_1810 0.80 +0.8 1,908,868 + Dsui_1810 0.88 -0.4 1,908,868 + Dsui_1810 0.88 -1.1 1,908,876 - -0.2 1,908,904 - -0.8 1,908,934 + -1.4 1,908,942 - -0.8 1,908,982 + Dsui_1811 0.13 -0.1 1,909,089 - Dsui_1811 0.31 -0.8 1,909,097 - Dsui_1811 0.32 -0.2 1,909,097 - Dsui_1811 0.32 -1.2 1,909,192 + Dsui_1811 0.48 -0.4 1,909,195 - Dsui_1811 0.48 +0.8 1,909,223 + Dsui_1811 0.53 -0.2 1,909,474 + -0.4 1,909,476 - -1.5 1,909,539 + -1.0 1,909,541 - -0.8 1,909,611 + -0.3 1,909,662 + -0.7 1,910,296 + -3.0
Or see this region's nucleotide sequence