Experiment: ALP with Cobalt chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1145 and Dsui_1146 are separated by 312 nucleotides Dsui_1146 and Dsui_1147 are separated by 194 nucleotides
Dsui_1145: Dsui_1145 - site-specific recombinase XerD, at 1,199,660 to 1,200,784
_1145
Dsui_1146: Dsui_1146 - high-affinity nickel permease, at 1,201,097 to 1,201,906
_1146
Dsui_1147: Dsui_1147 - PAS domain S-box, at 1,202,101 to 1,203,474
_1147
Position (kb)
1201
1202 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1200.101 kb on - strand, within Dsui_1145 at 1200.101 kb on - strand, within Dsui_1145 at 1200.129 kb on + strand, within Dsui_1145 at 1200.247 kb on + strand, within Dsui_1145 at 1200.255 kb on - strand, within Dsui_1145 at 1200.255 kb on - strand, within Dsui_1145 at 1200.258 kb on + strand, within Dsui_1145 at 1200.275 kb on - strand, within Dsui_1145 at 1200.276 kb on - strand, within Dsui_1145 at 1200.276 kb on - strand, within Dsui_1145 at 1200.328 kb on - strand, within Dsui_1145 at 1200.341 kb on - strand, within Dsui_1145 at 1200.354 kb on + strand, within Dsui_1145 at 1200.354 kb on + strand, within Dsui_1145 at 1200.400 kb on + strand, within Dsui_1145 at 1200.403 kb on + strand, within Dsui_1145 at 1200.437 kb on + strand, within Dsui_1145 at 1200.479 kb on + strand, within Dsui_1145 at 1200.487 kb on - strand, within Dsui_1145 at 1200.490 kb on - strand, within Dsui_1145 at 1200.495 kb on - strand, within Dsui_1145 at 1200.524 kb on + strand, within Dsui_1145 at 1200.568 kb on + strand, within Dsui_1145 at 1200.568 kb on + strand, within Dsui_1145 at 1200.588 kb on - strand, within Dsui_1145 at 1200.635 kb on - strand, within Dsui_1145 at 1200.681 kb on - strand at 1200.728 kb on + strand at 1200.732 kb on + strand at 1200.740 kb on - strand at 1200.740 kb on - strand at 1200.740 kb on - strand at 1200.742 kb on - strand at 1200.743 kb on + strand at 1200.751 kb on + strand at 1200.758 kb on + strand at 1200.780 kb on - strand at 1200.866 kb on + strand at 1200.867 kb on + strand at 1200.868 kb on - strand at 1200.874 kb on - strand at 1200.884 kb on + strand at 1201.002 kb on + strand at 1201.045 kb on + strand at 1201.095 kb on + strand at 1201.183 kb on + strand, within Dsui_1146 at 1201.285 kb on - strand, within Dsui_1146 at 1201.344 kb on + strand, within Dsui_1146 at 1201.457 kb on - strand, within Dsui_1146 at 1201.603 kb on + strand, within Dsui_1146 at 1201.619 kb on - strand, within Dsui_1146 at 1201.621 kb on + strand, within Dsui_1146 at 1201.639 kb on + strand, within Dsui_1146 at 1201.740 kb on + strand, within Dsui_1146 at 1201.776 kb on + strand, within Dsui_1146 at 1201.864 kb on - strand at 1201.910 kb on - strand at 1202.057 kb on + strand at 1202.226 kb on - strand at 1202.270 kb on + strand, within Dsui_1147 at 1202.361 kb on + strand, within Dsui_1147 at 1202.366 kb on + strand, within Dsui_1147 at 1202.366 kb on + strand, within Dsui_1147 at 1202.366 kb on + strand, within Dsui_1147 at 1202.366 kb on + strand, within Dsui_1147 at 1202.374 kb on - strand, within Dsui_1147 at 1202.381 kb on + strand, within Dsui_1147 at 1202.433 kb on + strand, within Dsui_1147 at 1202.481 kb on + strand at 1202.656 kb on - strand, within Dsui_1147 at 1202.728 kb on + strand, within Dsui_1147 at 1202.728 kb on + strand, within Dsui_1147 at 1202.736 kb on - strand, within Dsui_1147 at 1202.796 kb on + strand, within Dsui_1147 at 1202.810 kb on + strand, within Dsui_1147 at 1202.904 kb on - strand, within Dsui_1147
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cobalt chloride 0.25 mM remove 1,200,101 - Dsui_1145 0.39 +1.6 1,200,101 - Dsui_1145 0.39 +0.6 1,200,129 + Dsui_1145 0.42 -1.0 1,200,247 + Dsui_1145 0.52 -1.7 1,200,255 - Dsui_1145 0.53 +0.6 1,200,255 - Dsui_1145 0.53 +0.6 1,200,258 + Dsui_1145 0.53 +0.6 1,200,275 - Dsui_1145 0.55 -0.4 1,200,276 - Dsui_1145 0.55 -1.4 1,200,276 - Dsui_1145 0.55 -1.0 1,200,328 - Dsui_1145 0.59 +0.6 1,200,341 - Dsui_1145 0.61 -2.7 1,200,354 + Dsui_1145 0.62 -1.0 1,200,354 + Dsui_1145 0.62 -1.4 1,200,400 + Dsui_1145 0.66 +0.6 1,200,403 + Dsui_1145 0.66 -0.4 1,200,437 + Dsui_1145 0.69 +0.6 1,200,479 + Dsui_1145 0.73 +0.6 1,200,487 - Dsui_1145 0.74 -0.4 1,200,490 - Dsui_1145 0.74 +0.6 1,200,495 - Dsui_1145 0.74 -3.0 1,200,524 + Dsui_1145 0.77 -2.7 1,200,568 + Dsui_1145 0.81 -1.7 1,200,568 + Dsui_1145 0.81 +0.6 1,200,588 - Dsui_1145 0.82 -1.9 1,200,635 - Dsui_1145 0.87 -0.6 1,200,681 - -1.4 1,200,728 + +0.6 1,200,732 + +0.6 1,200,740 - +0.9 1,200,740 - +0.1 1,200,740 - +0.6 1,200,742 - +0.6 1,200,743 + -1.0 1,200,751 + +0.6 1,200,758 + -1.6 1,200,780 - +1.6 1,200,866 + +0.6 1,200,867 + -0.4 1,200,868 - +0.0 1,200,874 - -2.2 1,200,884 + -0.4 1,201,002 + +0.6 1,201,045 + -1.4 1,201,095 + -2.4 1,201,183 + Dsui_1146 0.11 +0.6 1,201,285 - Dsui_1146 0.23 -0.3 1,201,344 + Dsui_1146 0.30 -2.2 1,201,457 - Dsui_1146 0.44 -0.7 1,201,603 + Dsui_1146 0.62 +0.6 1,201,619 - Dsui_1146 0.64 -1.2 1,201,621 + Dsui_1146 0.65 -3.2 1,201,639 + Dsui_1146 0.67 -1.6 1,201,740 + Dsui_1146 0.79 +0.6 1,201,776 + Dsui_1146 0.84 -2.0 1,201,864 - -0.2 1,201,910 - +0.5 1,202,057 + +0.6 1,202,226 - -1.6 1,202,270 + Dsui_1147 0.12 +0.6 1,202,361 + Dsui_1147 0.19 -1.0 1,202,366 + Dsui_1147 0.19 -1.0 1,202,366 + Dsui_1147 0.19 -1.0 1,202,366 + Dsui_1147 0.19 +0.6 1,202,366 + Dsui_1147 0.19 -0.4 1,202,374 - Dsui_1147 0.20 -0.2 1,202,381 + Dsui_1147 0.20 +0.6 1,202,433 + Dsui_1147 0.24 +0.6 1,202,481 + -0.4 1,202,656 - Dsui_1147 0.40 -0.1 1,202,728 + Dsui_1147 0.46 +0.6 1,202,728 + Dsui_1147 0.46 -1.7 1,202,736 - Dsui_1147 0.46 -2.0 1,202,796 + Dsui_1147 0.51 -1.0 1,202,810 + Dsui_1147 0.52 -1.0 1,202,904 - Dsui_1147 0.58 -0.7
Or see this region's nucleotide sequence