Experiment: ALP with Cobalt chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0923 and Dsui_0924 are separated by 76 nucleotides Dsui_0924 and Dsui_0925 are separated by 2 nucleotides Dsui_0925 and Dsui_0926 are separated by 66 nucleotides
Dsui_0923: Dsui_0923 - putative iron-regulated membrane protein, at 951,058 to 952,206
_0923
Dsui_0924: Dsui_0924 - putative permease, at 952,283 to 953,743
_0924
Dsui_0925: Dsui_0925 - small redox-active disulfide protein 2, at 953,746 to 953,988
_0925
Dsui_0926: Dsui_0926 - fumarate hydratase, class II, at 954,055 to 955,446
_0926
Position (kb) 952
953
954 Strain fitness (log2 ratio) -2
-1
0
1 at 951.467 kb on - strand, within Dsui_0923 at 951.527 kb on + strand, within Dsui_0923 at 951.638 kb on - strand at 951.657 kb on - strand, within Dsui_0923 at 951.761 kb on - strand, within Dsui_0923 at 951.761 kb on - strand, within Dsui_0923 at 951.782 kb on - strand, within Dsui_0923 at 951.943 kb on - strand, within Dsui_0923 at 952.022 kb on + strand, within Dsui_0923 at 952.027 kb on + strand at 952.027 kb on + strand, within Dsui_0923 at 952.030 kb on - strand, within Dsui_0923 at 952.051 kb on + strand, within Dsui_0923 at 952.062 kb on + strand, within Dsui_0923 at 952.159 kb on - strand at 952.208 kb on - strand at 952.249 kb on + strand at 952.318 kb on - strand at 952.402 kb on - strand at 952.526 kb on - strand, within Dsui_0924 at 952.563 kb on + strand at 952.621 kb on + strand at 952.621 kb on + strand, within Dsui_0924 at 952.629 kb on - strand at 952.629 kb on - strand, within Dsui_0924 at 952.629 kb on - strand at 952.773 kb on - strand, within Dsui_0924 at 952.883 kb on - strand at 952.889 kb on + strand, within Dsui_0924 at 952.889 kb on + strand at 953.258 kb on - strand, within Dsui_0924 at 953.258 kb on - strand, within Dsui_0924 at 953.343 kb on - strand at 953.378 kb on + strand, within Dsui_0924 at 953.422 kb on + strand, within Dsui_0924 at 953.430 kb on + strand, within Dsui_0924 at 953.432 kb on - strand at 953.448 kb on + strand, within Dsui_0924 at 953.489 kb on + strand, within Dsui_0924 at 953.500 kb on + strand, within Dsui_0924 at 953.512 kb on - strand at 953.578 kb on - strand, within Dsui_0924 at 953.580 kb on + strand, within Dsui_0924 at 953.620 kb on + strand at 953.753 kb on + strand at 953.753 kb on + strand at 953.761 kb on - strand at 953.761 kb on - strand at 953.862 kb on - strand, within Dsui_0925 at 953.970 kb on + strand at 954.080 kb on + strand at 954.137 kb on + strand at 954.139 kb on + strand at 954.145 kb on - strand at 954.288 kb on + strand, within Dsui_0926 at 954.392 kb on + strand, within Dsui_0926 at 954.412 kb on + strand, within Dsui_0926 at 954.448 kb on - strand, within Dsui_0926 at 954.659 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cobalt chloride 0.25 mM remove 951,467 - Dsui_0923 0.36 +0.7 951,527 + Dsui_0923 0.41 -1.3 951,638 - +0.7 951,657 - Dsui_0923 0.52 -1.6 951,761 - Dsui_0923 0.61 -0.3 951,761 - Dsui_0923 0.61 +0.7 951,782 - Dsui_0923 0.63 -1.6 951,943 - Dsui_0923 0.77 -0.3 952,022 + Dsui_0923 0.84 -2.1 952,027 + -0.3 952,027 + Dsui_0923 0.84 -0.3 952,030 - Dsui_0923 0.85 -1.3 952,051 + Dsui_0923 0.86 -2.3 952,062 + Dsui_0923 0.87 -0.3 952,159 - +0.7 952,208 - +0.7 952,249 + -1.3 952,318 - +0.7 952,402 - -0.3 952,526 - Dsui_0924 0.17 -0.3 952,563 + +0.7 952,621 + -0.3 952,621 + Dsui_0924 0.23 +1.7 952,629 - +0.7 952,629 - Dsui_0924 0.24 -0.9 952,629 - -0.3 952,773 - Dsui_0924 0.34 -0.9 952,883 - -0.3 952,889 + Dsui_0924 0.41 -2.5 952,889 + +0.7 953,258 - Dsui_0924 0.67 -2.3 953,258 - Dsui_0924 0.67 +0.7 953,343 - -0.3 953,378 + Dsui_0924 0.75 -0.9 953,422 + Dsui_0924 0.78 -1.3 953,430 + Dsui_0924 0.79 -0.9 953,432 - -0.3 953,448 + Dsui_0924 0.80 -0.9 953,489 + Dsui_0924 0.83 -0.9 953,500 + Dsui_0924 0.83 -0.9 953,512 - +0.7 953,578 - Dsui_0924 0.89 +0.7 953,580 + Dsui_0924 0.89 +0.7 953,620 + -0.4 953,753 + -2.2 953,753 + -1.3 953,761 - -1.7 953,761 - -0.3 953,862 - Dsui_0925 0.48 -0.3 953,970 + -0.3 954,080 + -1.2 954,137 + -0.7 954,139 + -1.3 954,145 - -1.6 954,288 + Dsui_0926 0.17 -1.1 954,392 + Dsui_0926 0.24 +0.0 954,412 + Dsui_0926 0.26 -0.5 954,448 - Dsui_0926 0.28 -1.6 954,659 - +0.7
Or see this region's nucleotide sequence