Experiment: ALP with Cobalt chloride 0.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0124 and Dsui_0125 are separated by 4 nucleotides Dsui_0125 and Dsui_0126 are separated by 108 nucleotides Dsui_0126 and Dsui_0127 are separated by 160 nucleotides
Dsui_0124: Dsui_0124 - lactate dehydrogenase-like oxidoreductase, at 121,634 to 122,602
_0124
Dsui_0125: Dsui_0125 - NAD-dependent aldehyde dehydrogenase, at 122,607 to 123,344
_0125
Dsui_0126: Dsui_0126 - (4Fe-4S) cluster-containing protein, at 123,453 to 124,892
_0126
Dsui_0127: Dsui_0127 - transcriptional regulator, at 125,053 to 125,886
_0127
Position (kb)
123
124
125 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 122.519 kb on - strand at 122.526 kb on + strand at 122.534 kb on - strand at 122.605 kb on + strand at 122.655 kb on - strand at 122.675 kb on - strand at 122.872 kb on - strand, within Dsui_0125 at 122.896 kb on + strand at 123.218 kb on + strand at 123.241 kb on - strand, within Dsui_0125 at 123.272 kb on - strand at 123.282 kb on - strand at 123.287 kb on - strand at 123.315 kb on + strand at 123.317 kb on - strand at 123.331 kb on + strand at 123.360 kb on - strand at 123.395 kb on - strand at 123.427 kb on + strand at 123.446 kb on - strand at 123.451 kb on + strand at 123.459 kb on - strand at 123.493 kb on + strand at 123.655 kb on - strand, within Dsui_0126 at 123.682 kb on - strand, within Dsui_0126 at 123.697 kb on - strand, within Dsui_0126 at 123.770 kb on - strand, within Dsui_0126 at 123.827 kb on - strand, within Dsui_0126 at 123.828 kb on - strand, within Dsui_0126 at 123.839 kb on - strand, within Dsui_0126 at 123.869 kb on - strand, within Dsui_0126 at 123.899 kb on - strand, within Dsui_0126 at 124.007 kb on + strand, within Dsui_0126 at 124.007 kb on + strand at 124.035 kb on - strand, within Dsui_0126 at 124.035 kb on - strand, within Dsui_0126 at 124.092 kb on + strand, within Dsui_0126 at 124.111 kb on + strand at 124.127 kb on + strand, within Dsui_0126 at 124.220 kb on - strand, within Dsui_0126 at 124.276 kb on + strand, within Dsui_0126 at 124.286 kb on - strand at 124.322 kb on - strand, within Dsui_0126 at 124.332 kb on + strand at 124.348 kb on - strand, within Dsui_0126 at 124.396 kb on - strand, within Dsui_0126 at 124.396 kb on - strand, within Dsui_0126 at 124.533 kb on - strand, within Dsui_0126 at 124.543 kb on - strand, within Dsui_0126 at 124.567 kb on - strand, within Dsui_0126 at 124.740 kb on - strand, within Dsui_0126 at 124.759 kb on - strand at 124.781 kb on + strand at 124.785 kb on - strand at 124.828 kb on - strand at 124.848 kb on + strand at 124.900 kb on + strand at 124.900 kb on + strand at 124.900 kb on + strand at 124.900 kb on + strand at 124.900 kb on + strand at 124.900 kb on + strand at 124.900 kb on + strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.908 kb on - strand at 124.910 kb on + strand at 125.014 kb on - strand at 125.072 kb on + strand at 125.080 kb on - strand at 125.135 kb on + strand at 125.204 kb on + strand, within Dsui_0127 at 125.204 kb on + strand, within Dsui_0127 at 125.204 kb on + strand, within Dsui_0127 at 125.212 kb on - strand, within Dsui_0127 at 125.217 kb on + strand, within Dsui_0127 at 125.217 kb on + strand, within Dsui_0127 at 125.240 kb on - strand, within Dsui_0127 at 125.266 kb on + strand, within Dsui_0127 at 125.309 kb on + strand, within Dsui_0127 at 125.383 kb on - strand, within Dsui_0127 at 125.388 kb on - strand, within Dsui_0127 at 125.399 kb on - strand, within Dsui_0127 at 125.411 kb on + strand, within Dsui_0127 at 125.429 kb on + strand, within Dsui_0127 at 125.482 kb on - strand, within Dsui_0127 at 125.514 kb on + strand, within Dsui_0127 at 125.536 kb on - strand, within Dsui_0127 at 125.541 kb on - strand, within Dsui_0127 at 125.556 kb on + strand, within Dsui_0127 at 125.556 kb on + strand, within Dsui_0127 at 125.564 kb on - strand, within Dsui_0127 at 125.816 kb on + strand at 125.837 kb on + strand at 125.857 kb on + strand at 125.857 kb on + strand at 125.858 kb on - strand at 125.862 kb on + strand at 125.875 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cobalt chloride 0.25 mM remove 122,519 - +0.0 122,526 + +0.8 122,534 - -0.2 122,605 + +0.8 122,655 - -0.6 122,675 - -0.2 122,872 - Dsui_0125 0.36 -1.3 122,896 + +0.8 123,218 + +0.8 123,241 - Dsui_0125 0.86 -1.2 123,272 - -1.6 123,282 - -3.0 123,287 - -1.5 123,315 + +0.8 123,317 - -1.3 123,331 + -1.8 123,360 - -0.5 123,395 - +0.2 123,427 + -0.2 123,446 - +0.5 123,451 + -1.2 123,459 - -0.2 123,493 + -2.0 123,655 - Dsui_0126 0.14 -0.3 123,682 - Dsui_0126 0.16 +0.2 123,697 - Dsui_0126 0.17 -2.2 123,770 - Dsui_0126 0.22 -0.4 123,827 - Dsui_0126 0.26 -0.3 123,828 - Dsui_0126 0.26 -0.4 123,839 - Dsui_0126 0.27 -0.1 123,869 - Dsui_0126 0.29 -0.4 123,899 - Dsui_0126 0.31 -0.5 124,007 + Dsui_0126 0.38 -1.2 124,007 + +0.8 124,035 - Dsui_0126 0.40 -0.9 124,035 - Dsui_0126 0.40 -0.5 124,092 + Dsui_0126 0.44 -1.5 124,111 + +0.8 124,127 + Dsui_0126 0.47 -0.2 124,220 - Dsui_0126 0.53 -0.8 124,276 + Dsui_0126 0.57 -0.8 124,286 - -1.2 124,322 - Dsui_0126 0.60 -1.1 124,332 + +0.8 124,348 - Dsui_0126 0.62 +0.4 124,396 - Dsui_0126 0.65 +0.3 124,396 - Dsui_0126 0.65 -0.5 124,533 - Dsui_0126 0.75 -0.1 124,543 - Dsui_0126 0.76 -0.9 124,567 - Dsui_0126 0.77 -2.3 124,740 - Dsui_0126 0.89 -0.6 124,759 - -0.6 124,781 + +0.8 124,785 - +0.4 124,828 - -1.4 124,848 + +0.8 124,900 + +0.8 124,900 + -0.2 124,900 + -1.2 124,900 + +0.8 124,900 + +0.8 124,900 + -0.8 124,900 + -0.2 124,908 - -0.5 124,908 - -1.6 124,908 - -2.1 124,908 - -0.8 124,908 - -1.5 124,908 - +0.0 124,908 - -2.6 124,908 - +2.4 124,908 - -1.4 124,910 + -0.2 125,014 - +0.8 125,072 + -1.4 125,080 - -0.2 125,135 + -1.7 125,204 + Dsui_0127 0.18 -2.7 125,204 + Dsui_0127 0.18 -1.6 125,204 + Dsui_0127 0.18 -2.2 125,212 - Dsui_0127 0.19 -0.8 125,217 + Dsui_0127 0.20 -1.3 125,217 + Dsui_0127 0.20 -2.4 125,240 - Dsui_0127 0.22 -0.8 125,266 + Dsui_0127 0.26 -1.9 125,309 + Dsui_0127 0.31 -1.6 125,383 - Dsui_0127 0.40 -2.0 125,388 - Dsui_0127 0.40 -0.8 125,399 - Dsui_0127 0.41 -0.2 125,411 + Dsui_0127 0.43 -1.7 125,429 + Dsui_0127 0.45 -0.8 125,482 - Dsui_0127 0.51 -0.2 125,514 + Dsui_0127 0.55 -1.6 125,536 - Dsui_0127 0.58 +0.8 125,541 - Dsui_0127 0.59 -0.2 125,556 + Dsui_0127 0.60 -1.6 125,556 + Dsui_0127 0.60 -1.5 125,564 - Dsui_0127 0.61 -0.8 125,816 + -1.8 125,837 + -1.7 125,857 + -1.3 125,857 + -1.7 125,858 - -3.3 125,862 + -0.8 125,875 - -1.0
Or see this region's nucleotide sequence