Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22200

Experiment: D,L-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22190 and BWI76_RS22195 are separated by 57 nucleotidesBWI76_RS22195 and BWI76_RS22200 are separated by 8 nucleotidesBWI76_RS22200 and BWI76_RS22205 overlap by 4 nucleotidesBWI76_RS22205 and BWI76_RS22210 are separated by 236 nucleotides BWI76_RS22190: BWI76_RS22190 - hypothetical protein, at 4,627,874 to 4,628,066 _RS22190 BWI76_RS22195: BWI76_RS22195 - metal ABC transporter substrate-binding protein, at 4,628,124 to 4,629,002 _RS22195 BWI76_RS22200: BWI76_RS22200 - iron ABC transporter, at 4,629,011 to 4,629,874 _RS22200 BWI76_RS22205: BWI76_RS22205 - cation ABC transporter ATP-binding protein, at 4,629,871 to 4,630,548 _RS22205 BWI76_RS22210: BWI76_RS22210 - murein transglycosylase B, at 4,630,785 to 4,631,861 _RS22210 Position (kb) 4629 4630Strain fitness (log2 ratio) -3 -2 -1 0 1at 4628.150 kb on + strandat 4628.172 kb on + strandat 4628.223 kb on - strand, within BWI76_RS22195at 4628.227 kb on + strand, within BWI76_RS22195at 4628.235 kb on - strand, within BWI76_RS22195at 4628.278 kb on - strand, within BWI76_RS22195at 4628.301 kb on + strand, within BWI76_RS22195at 4628.325 kb on - strand, within BWI76_RS22195at 4628.432 kb on + strand, within BWI76_RS22195at 4628.432 kb on + strand, within BWI76_RS22195at 4628.440 kb on - strand, within BWI76_RS22195at 4628.442 kb on + strand, within BWI76_RS22195at 4628.458 kb on - strand, within BWI76_RS22195at 4628.481 kb on - strand, within BWI76_RS22195at 4628.529 kb on - strand, within BWI76_RS22195at 4628.531 kb on + strand, within BWI76_RS22195at 4628.620 kb on + strand, within BWI76_RS22195at 4628.628 kb on - strand, within BWI76_RS22195at 4628.633 kb on - strand, within BWI76_RS22195at 4628.718 kb on - strand, within BWI76_RS22195at 4628.733 kb on - strand, within BWI76_RS22195at 4628.756 kb on + strand, within BWI76_RS22195at 4628.813 kb on - strand, within BWI76_RS22195at 4628.819 kb on + strand, within BWI76_RS22195at 4628.819 kb on + strand, within BWI76_RS22195at 4628.827 kb on - strand, within BWI76_RS22195at 4628.827 kb on - strand, within BWI76_RS22195at 4628.827 kb on - strand, within BWI76_RS22195at 4628.901 kb on + strand, within BWI76_RS22195at 4628.902 kb on - strand, within BWI76_RS22195at 4629.105 kb on - strand, within BWI76_RS22200at 4629.105 kb on - strand, within BWI76_RS22200at 4629.148 kb on - strand, within BWI76_RS22200at 4629.389 kb on - strand, within BWI76_RS22200at 4629.396 kb on + strand, within BWI76_RS22200at 4629.429 kb on - strand, within BWI76_RS22200at 4629.429 kb on - strand, within BWI76_RS22200at 4629.849 kb on + strandat 4629.927 kb on - strandat 4630.123 kb on - strand, within BWI76_RS22205at 4630.233 kb on + strand, within BWI76_RS22205at 4630.241 kb on - strand, within BWI76_RS22205at 4630.497 kb on + strandat 4630.645 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Lactate (C)
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4,628,150 + -0.5
4,628,172 + -1.3
4,628,223 - BWI76_RS22195 0.11 -0.5
4,628,227 + BWI76_RS22195 0.12 +1.4
4,628,235 - BWI76_RS22195 0.13 -0.1
4,628,278 - BWI76_RS22195 0.18 +0.2
4,628,301 + BWI76_RS22195 0.20 +1.2
4,628,325 - BWI76_RS22195 0.23 +1.3
4,628,432 + BWI76_RS22195 0.35 -0.5
4,628,432 + BWI76_RS22195 0.35 -0.1
4,628,440 - BWI76_RS22195 0.36 -1.4
4,628,442 + BWI76_RS22195 0.36 +0.2
4,628,458 - BWI76_RS22195 0.38 -0.1
4,628,481 - BWI76_RS22195 0.41 +0.1
4,628,529 - BWI76_RS22195 0.46 -0.3
4,628,531 + BWI76_RS22195 0.46 +0.0
4,628,620 + BWI76_RS22195 0.56 +0.2
4,628,628 - BWI76_RS22195 0.57 +0.5
4,628,633 - BWI76_RS22195 0.58 -0.0
4,628,718 - BWI76_RS22195 0.68 -0.2
4,628,733 - BWI76_RS22195 0.69 -0.6
4,628,756 + BWI76_RS22195 0.72 -1.1
4,628,813 - BWI76_RS22195 0.78 -0.3
4,628,819 + BWI76_RS22195 0.79 -0.4
4,628,819 + BWI76_RS22195 0.79 +1.0
4,628,827 - BWI76_RS22195 0.80 +0.8
4,628,827 - BWI76_RS22195 0.80 -0.0
4,628,827 - BWI76_RS22195 0.80 +0.9
4,628,901 + BWI76_RS22195 0.88 -0.3
4,628,902 - BWI76_RS22195 0.89 -0.1
4,629,105 - BWI76_RS22200 0.11 -2.9
4,629,105 - BWI76_RS22200 0.11 +0.2
4,629,148 - BWI76_RS22200 0.16 -0.1
4,629,389 - BWI76_RS22200 0.44 -0.1
4,629,396 + BWI76_RS22200 0.45 +0.1
4,629,429 - BWI76_RS22200 0.48 +0.2
4,629,429 - BWI76_RS22200 0.48 +0.4
4,629,849 + -0.2
4,629,927 - -0.7
4,630,123 - BWI76_RS22205 0.37 +0.2
4,630,233 + BWI76_RS22205 0.53 +1.0
4,630,241 - BWI76_RS22205 0.55 -0.7
4,630,497 + +0.3
4,630,645 + -0.2

Or see this region's nucleotide sequence