Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3407 and panB are separated by 56 nucleotides panB and panC are separated by 76 nucleotides panC and Shewana3_3410 are separated by 291 nucleotides Shewana3_3410 and Shewana3_3411 are separated by 61 nucleotides
Shewana3_3407: Shewana3_3407 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (RefSeq), at 4,076,228 to 4,076,716
_3407
Shewana3_3408: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase (RefSeq), at 4,076,773 to 4,077,567
panB
Shewana3_3409: panC - pantoate--beta-alanine ligase (RefSeq), at 4,077,644 to 4,078,489
panC
Shewana3_3410: Shewana3_3410 - hypothetical protein (RefSeq), at 4,078,781 to 4,079,302
_3410
Shewana3_3411: Shewana3_3411 - peptidase S8 and S53, subtilisin, kexin, sedolisin (RefSeq), at 4,079,364 to 4,081,871
_3411
Position (kb)
4077
4078
4079 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 4076.890 kb on + strand, within panB at 4077.047 kb on + strand, within panB at 4077.081 kb on + strand, within panB at 4077.163 kb on + strand, within panB at 4077.433 kb on + strand, within panB at 4077.443 kb on + strand, within panB at 4078.547 kb on - strand at 4078.547 kb on - strand at 4078.547 kb on - strand at 4078.547 kb on - strand at 4078.552 kb on - strand at 4078.665 kb on + strand at 4079.051 kb on - strand, within Shewana3_3410 at 4079.370 kb on - strand at 4079.370 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 4,076,890 + panB Shewana3_3408 0.15 -1.7 4,077,047 + panB Shewana3_3408 0.34 -0.6 4,077,081 + panB Shewana3_3408 0.39 -3.0 4,077,163 + panB Shewana3_3408 0.49 -4.2 4,077,433 + panB Shewana3_3408 0.83 -3.5 4,077,443 + panB Shewana3_3408 0.84 -2.1 4,078,547 - -0.4 4,078,547 - +0.7 4,078,547 - +0.1 4,078,547 - -0.9 4,078,552 - +0.7 4,078,665 + +0.1 4,079,051 - Shewana3_3410 0.52 +1.0 4,079,370 - -0.2 4,079,370 - +0.5
Or see this region's nucleotide sequence