Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tyrA and Shewana3_3013 are separated by 62 nucleotides Shewana3_3013 and rplS are separated by 297 nucleotides rplS and trmD are separated by 34 nucleotides
Shewana3_3012: tyrA - bifunctional chorismate mutase/prephenate dehydrogenase (RefSeq), at 3,619,096 to 3,620,235
tyrA
Shewana3_3013: Shewana3_3013 - phospho-2-dehydro-3-deoxyheptonate aldolase (RefSeq), at 3,620,298 to 3,621,389
_3013
Shewana3_3014: rplS - 50S ribosomal protein L19 (RefSeq), at 3,621,687 to 3,622,040
rplS
Shewana3_3015: trmD - tRNA (guanine-N(1)-)-methyltransferase (RefSeq), at 3,622,075 to 3,622,821
trmD
Position (kb)
3620
3621
3622 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3619.844 kb on + strand, within tyrA at 3619.903 kb on - strand, within tyrA at 3620.112 kb on + strand, within tyrA at 3620.420 kb on + strand, within Shewana3_3013 at 3620.568 kb on - strand, within Shewana3_3013 at 3620.568 kb on - strand, within Shewana3_3013 at 3620.652 kb on + strand, within Shewana3_3013 at 3620.660 kb on - strand, within Shewana3_3013 at 3620.811 kb on - strand, within Shewana3_3013 at 3621.032 kb on - strand, within Shewana3_3013 at 3621.169 kb on - strand, within Shewana3_3013
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 3,619,844 + tyrA Shewana3_3012 0.66 -2.6 3,619,903 - tyrA Shewana3_3012 0.71 -3.5 3,620,112 + tyrA Shewana3_3012 0.89 -3.4 3,620,420 + Shewana3_3013 0.11 -3.5 3,620,568 - Shewana3_3013 0.25 -1.9 3,620,568 - Shewana3_3013 0.25 -2.9 3,620,652 + Shewana3_3013 0.32 -3.5 3,620,660 - Shewana3_3013 0.33 -0.5 3,620,811 - Shewana3_3013 0.47 -4.1 3,621,032 - Shewana3_3013 0.67 -2.0 3,621,169 - Shewana3_3013 0.80 -3.8
Or see this region's nucleotide sequence