Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2120 and Shewana3_2121 are separated by 164 nucleotides Shewana3_2121 and Shewana3_R0089 are separated by 293 nucleotides Shewana3_R0089 and Shewana3_R0090 are separated by 49 nucleotides Shewana3_R0090 and Shewana3_R0091 are separated by 23 nucleotides Shewana3_R0091 and Shewana3_2122 are separated by 325 nucleotides
Shewana3_2120: Shewana3_2120 - hypothetical protein (RefSeq), at 2,543,481 to 2,544,404
_2120
Shewana3_2121: Shewana3_2121 - aromatic amino acid aminotransferase (RefSeq), at 2,544,569 to 2,545,762
_2121
Shewana3_R0089: Shewana3_R0089 - tRNA-Leu (RefSeq), at 2,546,056 to 2,546,142
_R0089
Shewana3_R0090: Shewana3_R0090 - tRNA-Cys (RefSeq), at 2,546,192 to 2,546,265
_R0090
Shewana3_R0091: Shewana3_R0091 - tRNA-Gly (RefSeq), at 2,546,289 to 2,546,364
_R0091
Shewana3_2122: Shewana3_2122 - glyceraldehyde-3-phosphate dehydrogenase (RefSeq), at 2,546,690 to 2,548,123
_2122
Position (kb)
2544
2545
2546 Strain fitness (log2 ratio)
-2
-1
0
1 at 2543.640 kb on + strand, within Shewana3_2120 at 2543.645 kb on + strand, within Shewana3_2120 at 2543.803 kb on + strand, within Shewana3_2120 at 2544.258 kb on - strand, within Shewana3_2120 at 2544.333 kb on + strand at 2545.073 kb on + strand, within Shewana3_2121 at 2545.081 kb on - strand, within Shewana3_2121 at 2545.137 kb on + strand, within Shewana3_2121 at 2545.403 kb on - strand, within Shewana3_2121 at 2545.606 kb on - strand, within Shewana3_2121 at 2545.649 kb on - strand at 2545.695 kb on + strand at 2545.814 kb on - strand at 2545.826 kb on + strand at 2546.002 kb on - strand at 2546.042 kb on - strand at 2546.614 kb on + strand at 2546.624 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 2,543,640 + Shewana3_2120 0.17 -0.8 2,543,645 + Shewana3_2120 0.18 +0.1 2,543,803 + Shewana3_2120 0.35 -0.3 2,544,258 - Shewana3_2120 0.84 -1.3 2,544,333 + +0.6 2,545,073 + Shewana3_2121 0.42 -0.9 2,545,081 - Shewana3_2121 0.43 -1.0 2,545,137 + Shewana3_2121 0.48 -1.4 2,545,403 - Shewana3_2121 0.70 -2.2 2,545,606 - Shewana3_2121 0.87 -2.1 2,545,649 - -2.2 2,545,695 + -0.6 2,545,814 - -1.5 2,545,826 + -2.5 2,546,002 - -0.7 2,546,042 - +0.4 2,546,614 + +0.7 2,546,624 + -1.1
Or see this region's nucleotide sequence