Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1848 and Shewana3_1849 overlap by 19 nucleotides Shewana3_1849 and hisH are separated by 29 nucleotides hisH and Shewana3_1851 are separated by 145 nucleotides
Shewana3_1848: Shewana3_1848 - imidazole glycerol phosphate synthase subunit HisF (RefSeq), at 2,190,056 to 2,190,829
_1848
Shewana3_1849: Shewana3_1849 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq), at 2,190,811 to 2,191,548
_1849
Shewana3_1850: hisH - imidazole glycerol phosphate synthase subunit HisH (RefSeq), at 2,191,578 to 2,192,228
hisH
Shewana3_1851: Shewana3_1851 - imidazole glycerol-phosphate dehydratase/histidinol phosphatase (RefSeq), at 2,192,374 to 2,193,465
_1851
Position (kb)
2191
2192
2193 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2190.633 kb on + strand, within Shewana3_1848 at 2190.634 kb on + strand, within Shewana3_1848 at 2190.641 kb on - strand, within Shewana3_1848 at 2190.778 kb on - strand at 2190.826 kb on + strand at 2190.981 kb on + strand, within Shewana3_1849 at 2190.989 kb on - strand, within Shewana3_1849 at 2191.094 kb on - strand, within Shewana3_1849 at 2191.651 kb on + strand, within hisH at 2191.714 kb on - strand, within hisH at 2191.845 kb on + strand, within hisH at 2191.980 kb on - strand, within hisH at 2191.997 kb on + strand, within hisH at 2192.006 kb on - strand, within hisH at 2192.122 kb on + strand, within hisH at 2192.137 kb on - strand, within hisH at 2192.473 kb on - strand at 2192.490 kb on - strand, within Shewana3_1851 at 2192.499 kb on + strand, within Shewana3_1851 at 2192.507 kb on - strand, within Shewana3_1851 at 2192.507 kb on - strand, within Shewana3_1851 at 2192.507 kb on - strand, within Shewana3_1851 at 2192.558 kb on - strand, within Shewana3_1851 at 2192.705 kb on + strand, within Shewana3_1851 at 2192.711 kb on - strand, within Shewana3_1851 at 2192.718 kb on - strand, within Shewana3_1851 at 2192.723 kb on - strand, within Shewana3_1851 at 2192.736 kb on - strand, within Shewana3_1851 at 2192.742 kb on + strand, within Shewana3_1851 at 2192.921 kb on - strand, within Shewana3_1851 at 2192.988 kb on - strand, within Shewana3_1851 at 2193.080 kb on - strand, within Shewana3_1851 at 2193.086 kb on - strand, within Shewana3_1851 at 2193.166 kb on + strand, within Shewana3_1851 at 2193.223 kb on - strand, within Shewana3_1851
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 2,190,633 + Shewana3_1848 0.75 -4.4 2,190,634 + Shewana3_1848 0.75 -3.3 2,190,641 - Shewana3_1848 0.76 -3.2 2,190,778 - -3.9 2,190,826 + -3.0 2,190,981 + Shewana3_1849 0.23 -4.1 2,190,989 - Shewana3_1849 0.24 -1.5 2,191,094 - Shewana3_1849 0.38 -4.1 2,191,651 + hisH Shewana3_1850 0.11 -2.5 2,191,714 - hisH Shewana3_1850 0.21 -4.8 2,191,845 + hisH Shewana3_1850 0.41 -5.9 2,191,980 - hisH Shewana3_1850 0.62 -5.0 2,191,997 + hisH Shewana3_1850 0.64 -2.0 2,192,006 - hisH Shewana3_1850 0.66 -3.5 2,192,122 + hisH Shewana3_1850 0.84 -2.3 2,192,137 - hisH Shewana3_1850 0.86 -4.0 2,192,473 - -4.4 2,192,490 - Shewana3_1851 0.11 -4.4 2,192,499 + Shewana3_1851 0.11 -4.8 2,192,507 - Shewana3_1851 0.12 -4.8 2,192,507 - Shewana3_1851 0.12 -4.9 2,192,507 - Shewana3_1851 0.12 -1.6 2,192,558 - Shewana3_1851 0.17 -3.8 2,192,705 + Shewana3_1851 0.30 -3.3 2,192,711 - Shewana3_1851 0.31 -4.9 2,192,718 - Shewana3_1851 0.32 -5.0 2,192,723 - Shewana3_1851 0.32 -5.8 2,192,736 - Shewana3_1851 0.33 -5.2 2,192,742 + Shewana3_1851 0.34 -3.4 2,192,921 - Shewana3_1851 0.50 -4.7 2,192,988 - Shewana3_1851 0.56 -3.6 2,193,080 - Shewana3_1851 0.65 -4.0 2,193,086 - Shewana3_1851 0.65 -4.2 2,193,166 + Shewana3_1851 0.73 -1.7 2,193,223 - Shewana3_1851 0.78 -2.3
Or see this region's nucleotide sequence