Experiment: Carbon source Sucrose 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ispD and ispF are separated by 51 nucleotides ispF and Shewana3_1120 are separated by 29 nucleotides Shewana3_1120 and surE overlap by 4 nucleotides surE and Shewana3_1122 overlap by 4 nucleotides
Shewana3_1118: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (RefSeq), at 1,317,457 to 1,318,206
ispD
Shewana3_1119: ispF - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (RefSeq), at 1,318,258 to 1,318,737
ispF
Shewana3_1120: Shewana3_1120 - tRNA pseudouridine synthase D, TruD (RefSeq), at 1,318,767 to 1,319,894
_1120
Shewana3_1121: surE - stationary phase survival protein SurE (RefSeq), at 1,319,891 to 1,320,640
surE
Shewana3_1122: Shewana3_1122 - protein-L-isoaspartate O-methyltransferase (RefSeq), at 1,320,637 to 1,321,272
_1122
Position (kb)
1318
1319
1320 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1318.727 kb on + strand at 1318.941 kb on - strand, within Shewana3_1120 at 1319.026 kb on - strand, within Shewana3_1120 at 1319.066 kb on + strand, within Shewana3_1120 at 1319.074 kb on - strand, within Shewana3_1120 at 1319.146 kb on + strand, within Shewana3_1120 at 1319.262 kb on + strand, within Shewana3_1120 at 1319.331 kb on + strand, within Shewana3_1120 at 1319.333 kb on + strand, within Shewana3_1120 at 1319.350 kb on + strand, within Shewana3_1120 at 1319.584 kb on + strand, within Shewana3_1120 at 1319.876 kb on + strand at 1319.886 kb on + strand at 1319.978 kb on + strand, within surE at 1320.132 kb on - strand, within surE at 1320.232 kb on + strand, within surE at 1320.397 kb on + strand, within surE at 1320.433 kb on + strand, within surE at 1320.441 kb on - strand, within surE at 1320.578 kb on + strand at 1320.728 kb on + strand, within Shewana3_1122
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 5 mM remove 1,318,727 + -0.4 1,318,941 - Shewana3_1120 0.15 -1.0 1,319,026 - Shewana3_1120 0.23 -2.8 1,319,066 + Shewana3_1120 0.27 -1.1 1,319,074 - Shewana3_1120 0.27 -2.0 1,319,146 + Shewana3_1120 0.34 -0.9 1,319,262 + Shewana3_1120 0.44 +0.1 1,319,331 + Shewana3_1120 0.50 +0.1 1,319,333 + Shewana3_1120 0.50 +0.5 1,319,350 + Shewana3_1120 0.52 +0.0 1,319,584 + Shewana3_1120 0.72 -1.0 1,319,876 + -1.1 1,319,886 + -0.1 1,319,978 + surE Shewana3_1121 0.12 -2.6 1,320,132 - surE Shewana3_1121 0.32 -1.4 1,320,232 + surE Shewana3_1121 0.45 -2.6 1,320,397 + surE Shewana3_1121 0.67 -1.4 1,320,433 + surE Shewana3_1121 0.72 -3.1 1,320,441 - surE Shewana3_1121 0.73 -3.9 1,320,578 + -2.5 1,320,728 + Shewana3_1122 0.14 -1.4
Or see this region's nucleotide sequence