Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS30075 and RR42_RS30080 are separated by 82 nucleotides RR42_RS30080 and RR42_RS30085 are separated by 16 nucleotides RR42_RS30085 and RR42_RS30090 are separated by 475 nucleotides
RR42_RS30075: RR42_RS30075 - succinylglutamate desuccinylase, at 2,119,244 to 2,120,236
_RS30075
RR42_RS30080: RR42_RS30080 - MFS transporter, at 2,120,319 to 2,121,707
_RS30080
RR42_RS30085: RR42_RS30085 - hypothetical protein, at 2,121,724 to 2,122,089
_RS30085
RR42_RS30090: RR42_RS30090 - MFS transporter, at 2,122,565 to 2,123,836
_RS30090
Position (kb)
2120
2121
2122 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2119.415 kb on + strand, within RR42_RS30075 at 2119.415 kb on + strand, within RR42_RS30075 at 2119.416 kb on - strand, within RR42_RS30075 at 2119.416 kb on - strand, within RR42_RS30075 at 2119.416 kb on - strand, within RR42_RS30075 at 2119.416 kb on - strand, within RR42_RS30075 at 2119.523 kb on - strand, within RR42_RS30075 at 2119.523 kb on - strand, within RR42_RS30075 at 2119.613 kb on + strand, within RR42_RS30075 at 2119.614 kb on - strand, within RR42_RS30075 at 2119.614 kb on - strand, within RR42_RS30075 at 2119.690 kb on - strand, within RR42_RS30075 at 2119.690 kb on - strand, within RR42_RS30075 at 2119.739 kb on + strand, within RR42_RS30075 at 2119.739 kb on + strand, within RR42_RS30075 at 2119.811 kb on + strand, within RR42_RS30075 at 2120.013 kb on - strand, within RR42_RS30075 at 2120.037 kb on + strand, within RR42_RS30075 at 2120.109 kb on - strand, within RR42_RS30075 at 2120.232 kb on + strand at 2120.232 kb on + strand at 2120.232 kb on + strand at 2120.233 kb on - strand at 2120.235 kb on - strand at 2120.254 kb on + strand at 2120.255 kb on - strand at 2120.255 kb on - strand at 2120.418 kb on + strand at 2120.418 kb on + strand at 2120.418 kb on + strand at 2120.418 kb on + strand at 2120.418 kb on + strand at 2120.419 kb on - strand at 2120.419 kb on - strand at 2120.425 kb on + strand at 2120.426 kb on - strand at 2120.426 kb on - strand at 2120.457 kb on + strand at 2120.457 kb on + strand at 2120.457 kb on + strand at 2120.457 kb on + strand at 2120.458 kb on - strand, within RR42_RS30080 at 2120.458 kb on - strand, within RR42_RS30080 at 2120.516 kb on + strand, within RR42_RS30080 at 2120.565 kb on + strand, within RR42_RS30080 at 2120.566 kb on - strand, within RR42_RS30080 at 2120.623 kb on + strand, within RR42_RS30080 at 2120.624 kb on - strand, within RR42_RS30080 at 2120.824 kb on - strand, within RR42_RS30080 at 2120.824 kb on - strand, within RR42_RS30080 at 2120.824 kb on - strand, within RR42_RS30080 at 2121.083 kb on - strand, within RR42_RS30080 at 2121.105 kb on + strand, within RR42_RS30080 at 2121.106 kb on - strand, within RR42_RS30080 at 2121.128 kb on + strand, within RR42_RS30080 at 2121.141 kb on + strand, within RR42_RS30080 at 2121.141 kb on + strand, within RR42_RS30080 at 2121.141 kb on + strand, within RR42_RS30080 at 2121.229 kb on - strand, within RR42_RS30080 at 2121.229 kb on - strand, within RR42_RS30080 at 2121.229 kb on - strand, within RR42_RS30080 at 2121.278 kb on + strand, within RR42_RS30080 at 2121.283 kb on + strand, within RR42_RS30080 at 2121.283 kb on + strand, within RR42_RS30080 at 2121.283 kb on + strand, within RR42_RS30080 at 2121.284 kb on - strand, within RR42_RS30080 at 2121.284 kb on - strand, within RR42_RS30080 at 2121.284 kb on - strand, within RR42_RS30080 at 2121.318 kb on - strand, within RR42_RS30080 at 2121.452 kb on - strand, within RR42_RS30080 at 2121.524 kb on + strand, within RR42_RS30080 at 2121.528 kb on - strand, within RR42_RS30080 at 2121.528 kb on - strand, within RR42_RS30080 at 2121.620 kb on + strand at 2121.651 kb on + strand at 2121.775 kb on + strand, within RR42_RS30085 at 2121.776 kb on - strand, within RR42_RS30085 at 2121.940 kb on + strand, within RR42_RS30085 at 2121.941 kb on - strand, within RR42_RS30085 at 2121.941 kb on - strand, within RR42_RS30085 at 2122.083 kb on + strand at 2122.084 kb on - strand at 2122.084 kb on - strand at 2122.084 kb on - strand at 2122.109 kb on - strand at 2122.119 kb on + strand at 2122.119 kb on + strand at 2122.120 kb on - strand at 2122.123 kb on + strand at 2122.162 kb on + strand at 2122.239 kb on - strand at 2122.239 kb on - strand at 2122.374 kb on - strand at 2122.527 kb on - strand at 2122.535 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 2,119,415 + RR42_RS30075 0.17 +0.1 2,119,415 + RR42_RS30075 0.17 -1.2 2,119,416 - RR42_RS30075 0.17 -0.4 2,119,416 - RR42_RS30075 0.17 -2.8 2,119,416 - RR42_RS30075 0.17 -0.8 2,119,416 - RR42_RS30075 0.17 +0.5 2,119,523 - RR42_RS30075 0.28 -2.1 2,119,523 - RR42_RS30075 0.28 +0.0 2,119,613 + RR42_RS30075 0.37 -0.5 2,119,614 - RR42_RS30075 0.37 -0.4 2,119,614 - RR42_RS30075 0.37 -0.1 2,119,690 - RR42_RS30075 0.45 -0.0 2,119,690 - RR42_RS30075 0.45 -0.0 2,119,739 + RR42_RS30075 0.50 -0.4 2,119,739 + RR42_RS30075 0.50 -0.3 2,119,811 + RR42_RS30075 0.57 +0.3 2,120,013 - RR42_RS30075 0.77 -1.2 2,120,037 + RR42_RS30075 0.80 +0.5 2,120,109 - RR42_RS30075 0.87 -0.6 2,120,232 + +0.8 2,120,232 + -1.1 2,120,232 + -1.7 2,120,233 - +0.4 2,120,235 - +0.8 2,120,254 + -0.1 2,120,255 - -0.5 2,120,255 - +0.1 2,120,418 + +1.0 2,120,418 + -1.1 2,120,418 + -0.4 2,120,418 + -0.4 2,120,418 + -0.3 2,120,419 - -1.4 2,120,419 - -0.2 2,120,425 + +0.5 2,120,426 - +0.3 2,120,426 - +0.8 2,120,457 + +0.7 2,120,457 + -0.2 2,120,457 + -0.4 2,120,457 + -0.0 2,120,458 - RR42_RS30080 0.10 -0.8 2,120,458 - RR42_RS30080 0.10 -0.1 2,120,516 + RR42_RS30080 0.14 -0.1 2,120,565 + RR42_RS30080 0.18 -1.7 2,120,566 - RR42_RS30080 0.18 +0.0 2,120,623 + RR42_RS30080 0.22 -0.8 2,120,624 - RR42_RS30080 0.22 +0.2 2,120,824 - RR42_RS30080 0.36 -0.8 2,120,824 - RR42_RS30080 0.36 -0.7 2,120,824 - RR42_RS30080 0.36 +0.5 2,121,083 - RR42_RS30080 0.55 -0.3 2,121,105 + RR42_RS30080 0.57 -0.0 2,121,106 - RR42_RS30080 0.57 -0.6 2,121,128 + RR42_RS30080 0.58 -1.5 2,121,141 + RR42_RS30080 0.59 -0.4 2,121,141 + RR42_RS30080 0.59 -0.2 2,121,141 + RR42_RS30080 0.59 -0.1 2,121,229 - RR42_RS30080 0.66 +0.2 2,121,229 - RR42_RS30080 0.66 +0.8 2,121,229 - RR42_RS30080 0.66 +0.4 2,121,278 + RR42_RS30080 0.69 -1.8 2,121,283 + RR42_RS30080 0.69 +0.7 2,121,283 + RR42_RS30080 0.69 -0.4 2,121,283 + RR42_RS30080 0.69 -0.5 2,121,284 - RR42_RS30080 0.69 -1.0 2,121,284 - RR42_RS30080 0.69 +0.1 2,121,284 - RR42_RS30080 0.69 +0.1 2,121,318 - RR42_RS30080 0.72 +0.3 2,121,452 - RR42_RS30080 0.82 +0.6 2,121,524 + RR42_RS30080 0.87 -0.3 2,121,528 - RR42_RS30080 0.87 -1.0 2,121,528 - RR42_RS30080 0.87 -0.3 2,121,620 + -0.3 2,121,651 + +0.0 2,121,775 + RR42_RS30085 0.14 -0.5 2,121,776 - RR42_RS30085 0.14 +0.6 2,121,940 + RR42_RS30085 0.59 -1.0 2,121,941 - RR42_RS30085 0.59 -0.3 2,121,941 - RR42_RS30085 0.59 -0.3 2,122,083 + +0.9 2,122,084 - -0.1 2,122,084 - +1.3 2,122,084 - -0.3 2,122,109 - -0.8 2,122,119 + -0.2 2,122,119 + +0.0 2,122,120 - -0.4 2,122,123 + -0.7 2,122,162 + +1.1 2,122,239 - +0.1 2,122,239 - -0.5 2,122,374 - -0.6 2,122,527 - +1.2 2,122,535 - -1.1
Or see this region's nucleotide sequence