Experiment: R2A
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS16120 and RR42_RS16125 are separated by 57 nucleotides RR42_RS16125 and RR42_RS16130 overlap by 8 nucleotides RR42_RS16130 and RR42_RS16135 are separated by 5 nucleotides
RR42_RS16120: RR42_RS16120 - nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase, at 3,490,425 to 3,491,465
_RS16120
RR42_RS16125: RR42_RS16125 - Cob(I)yrinic acid a,c-diamide adenosyltransferase, at 3,491,523 to 3,492,164
_RS16125
RR42_RS16130: RR42_RS16130 - ABC transporter ATP-binding protein, at 3,492,157 to 3,492,993
_RS16130
RR42_RS16135: RR42_RS16135 - ABC transporter permease, at 3,492,999 to 3,494,006
_RS16135
Position (kb)
3491
3492
3493 Strain fitness (log2 ratio)
-2
-1
0
1 at 3490.901 kb on + strand, within RR42_RS16120 at 3490.901 kb on + strand, within RR42_RS16120 at 3490.908 kb on - strand, within RR42_RS16120 at 3490.941 kb on - strand, within RR42_RS16120 at 3491.245 kb on + strand, within RR42_RS16120 at 3491.468 kb on + strand at 3491.469 kb on - strand at 3491.515 kb on + strand at 3491.525 kb on - strand at 3491.525 kb on - strand at 3491.705 kb on - strand, within RR42_RS16125 at 3491.843 kb on - strand, within RR42_RS16125 at 3491.843 kb on - strand, within RR42_RS16125 at 3492.996 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A remove 3,490,901 + RR42_RS16120 0.46 -0.2 3,490,901 + RR42_RS16120 0.46 +1.1 3,490,908 - RR42_RS16120 0.46 -0.5 3,490,941 - RR42_RS16120 0.50 -0.5 3,491,245 + RR42_RS16120 0.79 -0.8 3,491,468 + +0.4 3,491,469 - +0.9 3,491,515 + +0.4 3,491,525 - -0.9 3,491,525 - +1.3 3,491,705 - RR42_RS16125 0.28 -0.3 3,491,843 - RR42_RS16125 0.50 -0.8 3,491,843 - RR42_RS16125 0.50 -1.9 3,492,996 + -0.6
Or see this region's nucleotide sequence