Experiment: Survival; Treatment=UGA synthetic gw pH7.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20930 and IAI47_20935 are separated by 325 nucleotides IAI47_20935 and IAI47_20940 are separated by 149 nucleotides IAI47_20940 and IAI47_20945 are separated by 71 nucleotides
IAI47_20930: IAI47_20930 - extracellular solute-binding protein, at 433,372 to 434,592
_20930
IAI47_20935: IAI47_20935 - ABC transporter ATP-binding protein, at 434,918 to 436,036
_20935
IAI47_20940: IAI47_20940 - hypothetical protein, at 436,186 to 436,743
_20940
IAI47_20945: IAI47_20945 - hypothetical protein, at 436,815 to 437,156
_20945
Position (kb) 434
435
436
437 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5 at 433.949 kb on + strand, within IAI47_20930 at 433.949 kb on + strand, within IAI47_20930 at 433.949 kb on + strand, within IAI47_20930 at 433.949 kb on + strand, within IAI47_20930 at 433.949 kb on + strand, within IAI47_20930 at 433.950 kb on - strand, within IAI47_20930 at 433.950 kb on - strand, within IAI47_20930 at 433.950 kb on - strand, within IAI47_20930 at 433.950 kb on - strand, within IAI47_20930 at 433.990 kb on - strand, within IAI47_20930 at 433.990 kb on - strand, within IAI47_20930 at 434.033 kb on + strand, within IAI47_20930 at 434.033 kb on + strand, within IAI47_20930 at 434.033 kb on + strand, within IAI47_20930 at 434.033 kb on + strand, within IAI47_20930 at 434.033 kb on + strand, within IAI47_20930 at 434.033 kb on + strand, within IAI47_20930 at 434.033 kb on + strand, within IAI47_20930 at 434.033 kb on + strand, within IAI47_20930 at 434.034 kb on - strand, within IAI47_20930 at 434.034 kb on - strand, within IAI47_20930 at 434.034 kb on - strand, within IAI47_20930 at 434.034 kb on - strand, within IAI47_20930 at 434.034 kb on - strand, within IAI47_20930 at 434.034 kb on - strand, within IAI47_20930 at 434.034 kb on - strand, within IAI47_20930 at 434.034 kb on - strand, within IAI47_20930 at 434.066 kb on + strand, within IAI47_20930 at 434.066 kb on + strand, within IAI47_20930 at 434.069 kb on + strand, within IAI47_20930 at 434.069 kb on + strand, within IAI47_20930 at 434.069 kb on + strand, within IAI47_20930 at 434.069 kb on + strand, within IAI47_20930 at 434.099 kb on + strand, within IAI47_20930 at 434.138 kb on + strand, within IAI47_20930 at 434.183 kb on + strand, within IAI47_20930 at 434.222 kb on + strand, within IAI47_20930 at 434.222 kb on + strand, within IAI47_20930 at 434.222 kb on + strand, within IAI47_20930 at 434.223 kb on - strand, within IAI47_20930 at 434.223 kb on - strand, within IAI47_20930 at 434.223 kb on - strand, within IAI47_20930 at 434.223 kb on - strand, within IAI47_20930 at 434.223 kb on - strand, within IAI47_20930 at 434.223 kb on - strand, within IAI47_20930 at 434.223 kb on - strand, within IAI47_20930 at 434.223 kb on - strand, within IAI47_20930 at 434.223 kb on - strand, within IAI47_20930 at 434.242 kb on - strand, within IAI47_20930 at 434.267 kb on + strand, within IAI47_20930 at 434.267 kb on + strand, within IAI47_20930 at 434.268 kb on - strand, within IAI47_20930 at 434.496 kb on + strand at 434.497 kb on - strand at 434.497 kb on - strand at 434.497 kb on - strand at 434.497 kb on - strand at 434.514 kb on + strand at 434.514 kb on + strand at 434.514 kb on + strand at 434.515 kb on - strand at 434.515 kb on - strand at 434.515 kb on - strand at 434.515 kb on - strand at 434.544 kb on + strand at 434.544 kb on + strand at 434.544 kb on + strand at 434.544 kb on + strand at 434.571 kb on + strand at 434.571 kb on + strand at 434.777 kb on - strand at 434.777 kb on - strand at 434.797 kb on + strand at 434.797 kb on + strand at 434.797 kb on + strand at 434.797 kb on + strand at 434.797 kb on + strand at 434.797 kb on + strand at 434.797 kb on + strand at 434.823 kb on - strand at 434.823 kb on - strand at 434.873 kb on - strand at 434.915 kb on - strand at 434.915 kb on - strand at 434.915 kb on - strand at 434.915 kb on - strand at 434.915 kb on - strand at 434.915 kb on - strand at 434.926 kb on + strand at 434.927 kb on - strand at 434.992 kb on + strand at 434.992 kb on + strand at 435.040 kb on + strand, within IAI47_20935 at 435.203 kb on - strand, within IAI47_20935 at 435.223 kb on - strand, within IAI47_20935 at 435.305 kb on - strand, within IAI47_20935 at 435.310 kb on + strand, within IAI47_20935 at 435.311 kb on - strand, within IAI47_20935 at 435.367 kb on + strand, within IAI47_20935 at 435.382 kb on + strand, within IAI47_20935 at 435.382 kb on + strand, within IAI47_20935 at 435.383 kb on - strand, within IAI47_20935 at 435.383 kb on - strand, within IAI47_20935 at 435.430 kb on + strand, within IAI47_20935 at 435.448 kb on + strand, within IAI47_20935 at 435.448 kb on + strand, within IAI47_20935 at 435.448 kb on + strand, within IAI47_20935 at 435.448 kb on + strand, within IAI47_20935 at 435.448 kb on + strand, within IAI47_20935 at 435.449 kb on - strand, within IAI47_20935 at 435.449 kb on - strand, within IAI47_20935 at 435.449 kb on - strand, within IAI47_20935 at 435.482 kb on + strand, within IAI47_20935 at 435.482 kb on + strand, within IAI47_20935 at 435.482 kb on + strand, within IAI47_20935 at 435.482 kb on + strand, within IAI47_20935 at 435.482 kb on + strand, within IAI47_20935 at 435.482 kb on + strand, within IAI47_20935 at 435.483 kb on - strand, within IAI47_20935 at 435.483 kb on - strand, within IAI47_20935 at 435.483 kb on - strand, within IAI47_20935 at 435.542 kb on + strand, within IAI47_20935 at 435.543 kb on - strand, within IAI47_20935 at 435.581 kb on + strand, within IAI47_20935 at 435.582 kb on - strand, within IAI47_20935 at 435.582 kb on - strand, within IAI47_20935 at 435.584 kb on + strand, within IAI47_20935 at 435.584 kb on + strand, within IAI47_20935 at 435.585 kb on - strand, within IAI47_20935 at 435.628 kb on + strand, within IAI47_20935 at 435.628 kb on + strand, within IAI47_20935 at 435.628 kb on + strand, within IAI47_20935 at 435.628 kb on + strand, within IAI47_20935 at 435.628 kb on + strand, within IAI47_20935 at 435.629 kb on - strand, within IAI47_20935 at 435.691 kb on - strand, within IAI47_20935 at 435.691 kb on - strand, within IAI47_20935 at 435.783 kb on + strand, within IAI47_20935 at 435.783 kb on + strand, within IAI47_20935 at 435.783 kb on + strand, within IAI47_20935 at 435.784 kb on - strand, within IAI47_20935 at 435.784 kb on - strand, within IAI47_20935 at 435.784 kb on - strand, within IAI47_20935 at 435.857 kb on + strand, within IAI47_20935 at 435.857 kb on + strand, within IAI47_20935 at 435.857 kb on + strand, within IAI47_20935 at 435.857 kb on + strand, within IAI47_20935 at 435.858 kb on - strand, within IAI47_20935 at 435.858 kb on - strand, within IAI47_20935 at 435.858 kb on - strand, within IAI47_20935 at 435.858 kb on - strand, within IAI47_20935 at 435.972 kb on + strand at 435.972 kb on + strand at 435.973 kb on - strand at 435.973 kb on - strand at 435.973 kb on - strand at 436.043 kb on + strand at 436.043 kb on + strand at 436.044 kb on - strand at 436.044 kb on - strand at 436.081 kb on + strand at 436.081 kb on + strand at 436.081 kb on + strand at 436.082 kb on - strand at 436.089 kb on - strand at 436.089 kb on - strand at 436.089 kb on - strand at 436.166 kb on + strand at 436.166 kb on + strand at 436.167 kb on - strand at 436.167 kb on - strand at 436.167 kb on - strand at 436.187 kb on + strand at 436.187 kb on + strand at 436.188 kb on - strand at 436.188 kb on - strand at 436.204 kb on - strand at 436.204 kb on - strand at 436.204 kb on - strand at 436.230 kb on + strand at 436.230 kb on + strand at 436.230 kb on + strand at 436.312 kb on - strand, within IAI47_20940 at 436.312 kb on - strand, within IAI47_20940 at 436.389 kb on + strand, within IAI47_20940 at 436.389 kb on + strand, within IAI47_20940 at 436.389 kb on + strand, within IAI47_20940 at 436.389 kb on + strand, within IAI47_20940 at 436.390 kb on - strand, within IAI47_20940 at 436.391 kb on + strand, within IAI47_20940 at 436.392 kb on - strand, within IAI47_20940 at 436.392 kb on - strand, within IAI47_20940 at 436.392 kb on - strand, within IAI47_20940 at 436.392 kb on - strand, within IAI47_20940 at 436.392 kb on - strand, within IAI47_20940 at 436.392 kb on - strand, within IAI47_20940 at 436.425 kb on + strand, within IAI47_20940 at 436.425 kb on + strand, within IAI47_20940 at 436.425 kb on + strand, within IAI47_20940 at 436.426 kb on - strand, within IAI47_20940 at 436.525 kb on + strand, within IAI47_20940 at 436.525 kb on + strand, within IAI47_20940 at 436.525 kb on + strand, within IAI47_20940 at 436.525 kb on + strand, within IAI47_20940 at 436.525 kb on + strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.526 kb on - strand, within IAI47_20940 at 436.529 kb on + strand, within IAI47_20940 at 436.529 kb on + strand, within IAI47_20940 at 436.529 kb on + strand, within IAI47_20940 at 436.530 kb on - strand, within IAI47_20940 at 436.530 kb on - strand, within IAI47_20940 at 436.530 kb on - strand, within IAI47_20940 at 436.530 kb on - strand, within IAI47_20940 at 436.530 kb on - strand, within IAI47_20940 at 436.690 kb on + strand at 436.691 kb on - strand at 436.716 kb on + strand at 436.716 kb on + strand at 436.716 kb on + strand at 436.716 kb on + strand at 436.717 kb on - strand at 436.717 kb on - strand at 436.723 kb on + strand at 436.724 kb on - strand at 436.736 kb on - strand at 436.760 kb on + strand at 436.761 kb on - strand at 436.761 kb on - strand at 436.767 kb on + strand at 436.767 kb on + strand at 436.816 kb on + strand at 436.816 kb on + strand at 436.816 kb on + strand at 436.831 kb on + strand at 436.832 kb on - strand at 436.835 kb on + strand at 436.835 kb on + strand at 436.836 kb on - strand at 436.836 kb on - strand at 436.836 kb on - strand at 436.836 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 433,949 + IAI47_20930 0.47 +0.8 433,949 + IAI47_20930 0.47 -1.9 433,949 + IAI47_20930 0.47 +1.9 433,949 + IAI47_20930 0.47 +0.0 433,949 + IAI47_20930 0.47 -0.0 433,950 - IAI47_20930 0.47 -1.6 433,950 - IAI47_20930 0.47 -1.1 433,950 - IAI47_20930 0.47 -1.9 433,950 - IAI47_20930 0.47 -0.4 433,990 - IAI47_20930 0.51 -1.8 433,990 - IAI47_20930 0.51 -0.5 434,033 + IAI47_20930 0.54 -1.6 434,033 + IAI47_20930 0.54 +0.2 434,033 + IAI47_20930 0.54 +1.5 434,033 + IAI47_20930 0.54 +0.2 434,033 + IAI47_20930 0.54 -0.9 434,033 + IAI47_20930 0.54 +1.3 434,033 + IAI47_20930 0.54 -1.1 434,033 + IAI47_20930 0.54 -1.0 434,034 - IAI47_20930 0.54 -1.1 434,034 - IAI47_20930 0.54 +2.5 434,034 - IAI47_20930 0.54 -0.7 434,034 - IAI47_20930 0.54 -0.1 434,034 - IAI47_20930 0.54 +0.6 434,034 - IAI47_20930 0.54 -0.0 434,034 - IAI47_20930 0.54 -3.9 434,034 - IAI47_20930 0.54 -0.1 434,066 + IAI47_20930 0.57 -1.1 434,066 + IAI47_20930 0.57 +0.3 434,069 + IAI47_20930 0.57 +0.2 434,069 + IAI47_20930 0.57 -0.5 434,069 + IAI47_20930 0.57 -3.2 434,069 + IAI47_20930 0.57 -1.9 434,099 + IAI47_20930 0.60 +2.0 434,138 + IAI47_20930 0.63 -1.1 434,183 + IAI47_20930 0.66 -0.0 434,222 + IAI47_20930 0.70 -1.4 434,222 + IAI47_20930 0.70 -4.0 434,222 + IAI47_20930 0.70 -2.6 434,223 - IAI47_20930 0.70 -0.5 434,223 - IAI47_20930 0.70 -2.7 434,223 - IAI47_20930 0.70 -0.5 434,223 - IAI47_20930 0.70 -2.1 434,223 - IAI47_20930 0.70 +0.2 434,223 - IAI47_20930 0.70 -0.2 434,223 - IAI47_20930 0.70 +1.6 434,223 - IAI47_20930 0.70 +3.2 434,223 - IAI47_20930 0.70 -2.7 434,242 - IAI47_20930 0.71 +0.3 434,267 + IAI47_20930 0.73 +0.4 434,267 + IAI47_20930 0.73 +1.7 434,268 - IAI47_20930 0.73 +1.8 434,496 + +0.3 434,497 - +0.8 434,497 - +0.9 434,497 - -0.8 434,497 - +0.0 434,514 + +1.0 434,514 + +0.1 434,514 + +0.1 434,515 - -1.4 434,515 - +3.5 434,515 - +1.2 434,515 - -1.6 434,544 + -0.8 434,544 + +1.6 434,544 + +0.4 434,544 + -1.4 434,571 + +1.6 434,571 + +1.6 434,777 - +1.2 434,777 - +0.2 434,797 + -1.0 434,797 + +4.0 434,797 + -2.6 434,797 + +3.7 434,797 + -0.3 434,797 + -1.4 434,797 + +1.5 434,823 - -1.1 434,823 - -1.9 434,873 - +1.2 434,915 - -0.9 434,915 - +1.0 434,915 - -2.1 434,915 - -1.0 434,915 - -1.6 434,915 - -0.6 434,926 + +1.1 434,927 - +5.2 434,992 + +1.4 434,992 + -1.6 435,040 + IAI47_20935 0.11 -1.9 435,203 - IAI47_20935 0.25 -3.2 435,223 - IAI47_20935 0.27 -0.4 435,305 - IAI47_20935 0.35 +1.2 435,310 + IAI47_20935 0.35 +0.4 435,311 - IAI47_20935 0.35 -1.7 435,367 + IAI47_20935 0.40 -0.4 435,382 + IAI47_20935 0.41 -0.8 435,382 + IAI47_20935 0.41 +2.1 435,383 - IAI47_20935 0.42 -2.4 435,383 - IAI47_20935 0.42 +0.2 435,430 + IAI47_20935 0.46 +0.8 435,448 + IAI47_20935 0.47 -3.2 435,448 + IAI47_20935 0.47 -0.4 435,448 + IAI47_20935 0.47 -0.2 435,448 + IAI47_20935 0.47 +0.0 435,448 + IAI47_20935 0.47 -0.5 435,449 - IAI47_20935 0.47 -0.4 435,449 - IAI47_20935 0.47 -0.2 435,449 - IAI47_20935 0.47 -0.7 435,482 + IAI47_20935 0.50 +0.2 435,482 + IAI47_20935 0.50 -0.0 435,482 + IAI47_20935 0.50 -0.4 435,482 + IAI47_20935 0.50 -0.4 435,482 + IAI47_20935 0.50 +0.0 435,482 + IAI47_20935 0.50 +1.2 435,483 - IAI47_20935 0.50 -1.4 435,483 - IAI47_20935 0.50 +1.6 435,483 - IAI47_20935 0.50 +0.5 435,542 + IAI47_20935 0.56 -2.5 435,543 - IAI47_20935 0.56 +1.2 435,581 + IAI47_20935 0.59 +2.6 435,582 - IAI47_20935 0.59 +0.8 435,582 - IAI47_20935 0.59 +1.2 435,584 + IAI47_20935 0.60 -2.1 435,584 + IAI47_20935 0.60 -1.4 435,585 - IAI47_20935 0.60 +1.2 435,628 + IAI47_20935 0.63 +1.3 435,628 + IAI47_20935 0.63 -1.7 435,628 + IAI47_20935 0.63 -0.8 435,628 + IAI47_20935 0.63 -1.0 435,628 + IAI47_20935 0.63 -1.0 435,629 - IAI47_20935 0.64 +0.7 435,691 - IAI47_20935 0.69 +0.1 435,691 - IAI47_20935 0.69 -0.8 435,783 + IAI47_20935 0.77 -1.0 435,783 + IAI47_20935 0.77 -1.2 435,783 + IAI47_20935 0.77 +0.2 435,784 - IAI47_20935 0.77 -0.6 435,784 - IAI47_20935 0.77 -0.8 435,784 - IAI47_20935 0.77 -1.7 435,857 + IAI47_20935 0.84 -0.2 435,857 + IAI47_20935 0.84 +0.6 435,857 + IAI47_20935 0.84 +1.1 435,857 + IAI47_20935 0.84 +0.2 435,858 - IAI47_20935 0.84 +0.5 435,858 - IAI47_20935 0.84 +1.2 435,858 - IAI47_20935 0.84 +0.3 435,858 - IAI47_20935 0.84 +1.8 435,972 + +0.1 435,972 + +0.0 435,973 - -0.6 435,973 - -0.5 435,973 - -1.0 436,043 + +1.7 436,043 + +3.4 436,044 - +0.8 436,044 - -1.2 436,081 + -1.1 436,081 + -0.2 436,081 + -1.9 436,082 - -1.4 436,089 - -0.5 436,089 - +0.6 436,089 - -0.4 436,166 + +0.2 436,166 + -0.5 436,167 - -0.3 436,167 - -0.4 436,167 - -2.0 436,187 + +0.6 436,187 + +1.2 436,188 - +1.2 436,188 - -0.8 436,204 - -0.7 436,204 - -0.8 436,204 - +2.5 436,230 + -0.9 436,230 + +0.5 436,230 + -0.2 436,312 - IAI47_20940 0.23 +1.2 436,312 - IAI47_20940 0.23 -0.7 436,389 + IAI47_20940 0.36 -0.8 436,389 + IAI47_20940 0.36 -2.2 436,389 + IAI47_20940 0.36 -1.1 436,389 + IAI47_20940 0.36 -0.1 436,390 - IAI47_20940 0.37 -1.6 436,391 + IAI47_20940 0.37 -1.5 436,392 - IAI47_20940 0.37 -0.2 436,392 - IAI47_20940 0.37 -0.4 436,392 - IAI47_20940 0.37 +1.6 436,392 - IAI47_20940 0.37 +0.1 436,392 - IAI47_20940 0.37 -0.3 436,392 - IAI47_20940 0.37 +1.9 436,425 + IAI47_20940 0.43 +0.5 436,425 + IAI47_20940 0.43 -1.1 436,425 + IAI47_20940 0.43 -2.2 436,426 - IAI47_20940 0.43 -0.0 436,525 + IAI47_20940 0.61 +2.5 436,525 + IAI47_20940 0.61 +0.0 436,525 + IAI47_20940 0.61 +0.4 436,525 + IAI47_20940 0.61 +0.6 436,525 + IAI47_20940 0.61 +0.9 436,526 - IAI47_20940 0.61 -0.8 436,526 - IAI47_20940 0.61 -0.8 436,526 - IAI47_20940 0.61 -0.7 436,526 - IAI47_20940 0.61 +1.4 436,526 - IAI47_20940 0.61 +0.8 436,526 - IAI47_20940 0.61 -2.2 436,526 - IAI47_20940 0.61 +0.4 436,526 - IAI47_20940 0.61 -0.4 436,529 + IAI47_20940 0.61 -0.4 436,529 + IAI47_20940 0.61 +0.4 436,529 + IAI47_20940 0.61 -2.0 436,530 - IAI47_20940 0.62 +1.2 436,530 - IAI47_20940 0.62 -2.2 436,530 - IAI47_20940 0.62 +0.9 436,530 - IAI47_20940 0.62 +0.4 436,530 - IAI47_20940 0.62 -0.4 436,690 + +1.5 436,691 - +1.9 436,716 + -3.1 436,716 + -0.5 436,716 + +0.5 436,716 + +1.0 436,717 - -0.2 436,717 - -0.2 436,723 + -0.4 436,724 - +0.7 436,736 - -2.9 436,760 + -2.4 436,761 - -1.3 436,761 - -0.7 436,767 + +0.4 436,767 + -0.1 436,816 + +1.0 436,816 + +1.1 436,816 + -0.8 436,831 + +0.5 436,832 - -2.2 436,835 + -1.6 436,835 + +1.4 436,836 - -2.1 436,836 - -1.8 436,836 - -0.1 436,836 - +1.2
Or see this region's nucleotide sequence