Experiment: Survival; Treatment=UGA synthetic gw pH7.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_19965 and IAI47_19970 are separated by 177 nucleotides IAI47_19970 and IAI47_19975 are separated by 216 nucleotides
IAI47_19965: IAI47_19965 - ferric-rhodotorulic acid/ferric-coprogen receptor FhuE, at 220,754 to 222,976
_19965
IAI47_19970: IAI47_19970 - amidohydrolase, at 223,154 to 224,293
_19970
IAI47_19975: IAI47_19975 - MFS transporter, at 224,510 to 225,736
_19975
Position (kb) 223
224
225 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 222.206 kb on + strand, within IAI47_19965 at 222.207 kb on - strand, within IAI47_19965 at 222.207 kb on - strand, within IAI47_19965 at 222.231 kb on + strand, within IAI47_19965 at 222.231 kb on + strand, within IAI47_19965 at 222.231 kb on + strand, within IAI47_19965 at 222.231 kb on + strand, within IAI47_19965 at 222.232 kb on - strand, within IAI47_19965 at 222.232 kb on - strand, within IAI47_19965 at 222.232 kb on - strand, within IAI47_19965 at 222.232 kb on - strand, within IAI47_19965 at 222.232 kb on - strand, within IAI47_19965 at 222.259 kb on + strand, within IAI47_19965 at 222.259 kb on + strand, within IAI47_19965 at 222.260 kb on - strand, within IAI47_19965 at 222.260 kb on - strand, within IAI47_19965 at 222.260 kb on - strand, within IAI47_19965 at 222.260 kb on - strand, within IAI47_19965 at 222.260 kb on - strand, within IAI47_19965 at 222.260 kb on - strand, within IAI47_19965 at 222.281 kb on + strand, within IAI47_19965 at 222.308 kb on + strand, within IAI47_19965 at 222.308 kb on + strand, within IAI47_19965 at 222.317 kb on + strand, within IAI47_19965 at 222.317 kb on + strand, within IAI47_19965 at 222.318 kb on - strand, within IAI47_19965 at 222.318 kb on - strand, within IAI47_19965 at 222.318 kb on - strand, within IAI47_19965 at 222.318 kb on - strand, within IAI47_19965 at 222.319 kb on + strand, within IAI47_19965 at 222.319 kb on + strand, within IAI47_19965 at 222.319 kb on + strand, within IAI47_19965 at 222.319 kb on + strand, within IAI47_19965 at 222.319 kb on + strand, within IAI47_19965 at 222.320 kb on - strand, within IAI47_19965 at 222.320 kb on - strand, within IAI47_19965 at 222.320 kb on - strand, within IAI47_19965 at 222.365 kb on + strand, within IAI47_19965 at 222.365 kb on + strand, within IAI47_19965 at 222.366 kb on - strand, within IAI47_19965 at 222.366 kb on - strand, within IAI47_19965 at 222.393 kb on - strand, within IAI47_19965 at 222.521 kb on + strand, within IAI47_19965 at 222.521 kb on + strand, within IAI47_19965 at 222.521 kb on + strand, within IAI47_19965 at 222.522 kb on - strand, within IAI47_19965 at 222.522 kb on - strand, within IAI47_19965 at 222.522 kb on - strand, within IAI47_19965 at 222.522 kb on - strand, within IAI47_19965 at 222.522 kb on - strand, within IAI47_19965 at 222.522 kb on - strand, within IAI47_19965 at 222.522 kb on - strand, within IAI47_19965 at 222.522 kb on - strand, within IAI47_19965 at 222.617 kb on + strand, within IAI47_19965 at 222.617 kb on + strand, within IAI47_19965 at 222.618 kb on - strand, within IAI47_19965 at 222.677 kb on - strand, within IAI47_19965 at 222.688 kb on + strand, within IAI47_19965 at 222.688 kb on + strand, within IAI47_19965 at 222.688 kb on + strand, within IAI47_19965 at 222.689 kb on - strand, within IAI47_19965 at 222.689 kb on - strand, within IAI47_19965 at 222.689 kb on - strand, within IAI47_19965 at 222.689 kb on - strand, within IAI47_19965 at 222.689 kb on - strand, within IAI47_19965 at 222.692 kb on + strand, within IAI47_19965 at 222.693 kb on - strand, within IAI47_19965 at 222.693 kb on - strand at 222.812 kb on + strand at 222.812 kb on + strand at 222.812 kb on + strand at 222.812 kb on + strand at 222.812 kb on + strand at 222.812 kb on + strand at 222.813 kb on - strand at 222.813 kb on - strand at 222.813 kb on - strand at 222.813 kb on - strand at 222.813 kb on - strand at 222.813 kb on - strand at 222.813 kb on - strand at 222.828 kb on + strand at 222.828 kb on + strand at 222.880 kb on + strand at 222.880 kb on + strand at 222.881 kb on - strand at 222.899 kb on + strand at 222.899 kb on + strand at 222.930 kb on - strand at 222.966 kb on - strand at 222.966 kb on - strand at 222.981 kb on + strand at 222.981 kb on + strand at 222.981 kb on + strand at 222.982 kb on - strand at 222.989 kb on + strand at 222.990 kb on - strand at 222.990 kb on - strand at 223.033 kb on + strand at 223.033 kb on + strand at 223.033 kb on + strand at 223.033 kb on + strand at 223.033 kb on + strand at 223.033 kb on + strand at 223.033 kb on + strand at 223.034 kb on - strand at 223.034 kb on - strand at 223.034 kb on - strand at 223.034 kb on - strand at 223.034 kb on - strand at 223.034 kb on - strand at 223.052 kb on + strand at 223.052 kb on + strand at 223.053 kb on - strand at 223.053 kb on - strand at 223.060 kb on - strand at 223.203 kb on + strand at 223.204 kb on - strand at 223.204 kb on - strand at 223.204 kb on - strand at 223.314 kb on + strand, within IAI47_19970 at 223.314 kb on + strand, within IAI47_19970 at 223.314 kb on + strand, within IAI47_19970 at 223.315 kb on - strand, within IAI47_19970 at 223.315 kb on - strand, within IAI47_19970 at 223.315 kb on - strand, within IAI47_19970 at 223.315 kb on - strand, within IAI47_19970 at 223.368 kb on + strand, within IAI47_19970 at 223.369 kb on - strand, within IAI47_19970 at 223.369 kb on - strand, within IAI47_19970 at 223.417 kb on + strand, within IAI47_19970 at 223.417 kb on + strand, within IAI47_19970 at 223.418 kb on - strand, within IAI47_19970 at 223.418 kb on - strand, within IAI47_19970 at 223.420 kb on + strand, within IAI47_19970 at 223.420 kb on + strand, within IAI47_19970 at 223.421 kb on - strand, within IAI47_19970 at 223.483 kb on + strand, within IAI47_19970 at 223.548 kb on - strand, within IAI47_19970 at 223.548 kb on - strand, within IAI47_19970 at 223.591 kb on + strand, within IAI47_19970 at 223.592 kb on - strand, within IAI47_19970 at 223.592 kb on - strand, within IAI47_19970 at 223.646 kb on + strand, within IAI47_19970 at 223.681 kb on + strand, within IAI47_19970 at 223.681 kb on + strand, within IAI47_19970 at 223.682 kb on - strand, within IAI47_19970 at 223.682 kb on - strand, within IAI47_19970 at 223.779 kb on + strand, within IAI47_19970 at 223.780 kb on - strand, within IAI47_19970 at 223.780 kb on - strand, within IAI47_19970 at 224.214 kb on + strand at 224.214 kb on + strand at 224.214 kb on + strand at 224.214 kb on + strand at 224.214 kb on + strand at 224.215 kb on - strand at 224.215 kb on - strand at 224.215 kb on - strand at 224.215 kb on - strand at 224.215 kb on - strand at 224.279 kb on + strand at 224.279 kb on + strand at 224.279 kb on + strand at 224.296 kb on + strand at 224.297 kb on - strand at 224.297 kb on - strand at 224.297 kb on - strand at 224.315 kb on + strand at 224.316 kb on - strand at 224.324 kb on + strand at 224.375 kb on + strand at 224.375 kb on + strand at 224.375 kb on + strand at 224.375 kb on + strand at 224.376 kb on - strand at 224.399 kb on + strand at 224.400 kb on - strand at 224.407 kb on + strand at 224.435 kb on + strand at 224.435 kb on + strand at 224.435 kb on + strand at 224.435 kb on + strand at 224.435 kb on + strand at 224.436 kb on - strand at 224.504 kb on + strand at 224.504 kb on + strand at 224.504 kb on + strand at 224.505 kb on - strand at 224.512 kb on - strand at 224.594 kb on + strand at 224.594 kb on + strand at 224.594 kb on + strand at 224.595 kb on - strand at 224.599 kb on + strand at 224.600 kb on - strand at 224.625 kb on + strand at 224.626 kb on - strand at 224.736 kb on + strand, within IAI47_19975 at 224.802 kb on + strand, within IAI47_19975 at 224.802 kb on + strand, within IAI47_19975 at 224.802 kb on + strand, within IAI47_19975 at 224.802 kb on + strand, within IAI47_19975 at 224.802 kb on + strand, within IAI47_19975 at 224.802 kb on + strand, within IAI47_19975 at 224.802 kb on + strand, within IAI47_19975 at 224.802 kb on + strand, within IAI47_19975 at 224.802 kb on + strand, within IAI47_19975 at 224.802 kb on + strand, within IAI47_19975 at 224.803 kb on - strand, within IAI47_19975 at 224.803 kb on - strand, within IAI47_19975 at 224.803 kb on - strand, within IAI47_19975 at 224.803 kb on - strand, within IAI47_19975 at 224.803 kb on - strand, within IAI47_19975 at 224.803 kb on - strand, within IAI47_19975 at 224.876 kb on + strand, within IAI47_19975 at 224.876 kb on + strand, within IAI47_19975 at 224.877 kb on - strand, within IAI47_19975 at 224.877 kb on - strand, within IAI47_19975 at 224.877 kb on - strand, within IAI47_19975 at 224.877 kb on - strand, within IAI47_19975 at 224.906 kb on + strand, within IAI47_19975 at 224.927 kb on - strand, within IAI47_19975 at 224.927 kb on - strand, within IAI47_19975 at 224.927 kb on - strand, within IAI47_19975 at 224.960 kb on + strand, within IAI47_19975 at 224.960 kb on + strand, within IAI47_19975 at 224.961 kb on - strand, within IAI47_19975 at 224.961 kb on - strand, within IAI47_19975 at 224.961 kb on - strand, within IAI47_19975 at 224.961 kb on - strand, within IAI47_19975 at 224.961 kb on - strand, within IAI47_19975 at 224.961 kb on - strand, within IAI47_19975 at 224.970 kb on + strand, within IAI47_19975 at 224.970 kb on + strand, within IAI47_19975 at 224.971 kb on - strand, within IAI47_19975 at 224.971 kb on - strand, within IAI47_19975 at 224.971 kb on - strand, within IAI47_19975 at 224.971 kb on - strand, within IAI47_19975 at 225.024 kb on - strand, within IAI47_19975 at 225.024 kb on - strand, within IAI47_19975 at 225.098 kb on + strand, within IAI47_19975 at 225.099 kb on - strand, within IAI47_19975 at 225.106 kb on - strand, within IAI47_19975 at 225.171 kb on + strand, within IAI47_19975 at 225.171 kb on + strand, within IAI47_19975 at 225.171 kb on + strand, within IAI47_19975 at 225.171 kb on + strand, within IAI47_19975 at 225.171 kb on + strand, within IAI47_19975 at 225.171 kb on + strand, within IAI47_19975 at 225.171 kb on + strand, within IAI47_19975 at 225.172 kb on - strand, within IAI47_19975 at 225.172 kb on - strand, within IAI47_19975 at 225.172 kb on - strand, within IAI47_19975 at 225.172 kb on - strand, within IAI47_19975 at 225.172 kb on - strand, within IAI47_19975 at 225.172 kb on - strand, within IAI47_19975 at 225.176 kb on + strand, within IAI47_19975 at 225.176 kb on + strand, within IAI47_19975 at 225.177 kb on - strand, within IAI47_19975 at 225.177 kb on - strand, within IAI47_19975 at 225.177 kb on - strand, within IAI47_19975
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 222,206 + IAI47_19965 0.65 -1.7 222,207 - IAI47_19965 0.65 +0.2 222,207 - IAI47_19965 0.65 +0.0 222,231 + IAI47_19965 0.66 +0.7 222,231 + IAI47_19965 0.66 +1.8 222,231 + IAI47_19965 0.66 +0.4 222,231 + IAI47_19965 0.66 -0.1 222,232 - IAI47_19965 0.66 -0.4 222,232 - IAI47_19965 0.66 +1.7 222,232 - IAI47_19965 0.66 -1.6 222,232 - IAI47_19965 0.66 +0.2 222,232 - IAI47_19965 0.66 +1.2 222,259 + IAI47_19965 0.68 +3.5 222,259 + IAI47_19965 0.68 +1.2 222,260 - IAI47_19965 0.68 -2.4 222,260 - IAI47_19965 0.68 -1.8 222,260 - IAI47_19965 0.68 -2.7 222,260 - IAI47_19965 0.68 +0.1 222,260 - IAI47_19965 0.68 +0.5 222,260 - IAI47_19965 0.68 -0.6 222,281 + IAI47_19965 0.69 -1.9 222,308 + IAI47_19965 0.70 -0.2 222,308 + IAI47_19965 0.70 -1.6 222,317 + IAI47_19965 0.70 +0.7 222,317 + IAI47_19965 0.70 -0.6 222,318 - IAI47_19965 0.70 -2.2 222,318 - IAI47_19965 0.70 -0.9 222,318 - IAI47_19965 0.70 +1.6 222,318 - IAI47_19965 0.70 -1.1 222,319 + IAI47_19965 0.70 +2.2 222,319 + IAI47_19965 0.70 +0.8 222,319 + IAI47_19965 0.70 +0.0 222,319 + IAI47_19965 0.70 +2.2 222,319 + IAI47_19965 0.70 -2.9 222,320 - IAI47_19965 0.70 -0.1 222,320 - IAI47_19965 0.70 +4.4 222,320 - IAI47_19965 0.70 -0.0 222,365 + IAI47_19965 0.72 -1.8 222,365 + IAI47_19965 0.72 +0.5 222,366 - IAI47_19965 0.73 -0.4 222,366 - IAI47_19965 0.73 -1.9 222,393 - IAI47_19965 0.74 -1.4 222,521 + IAI47_19965 0.79 -1.8 222,521 + IAI47_19965 0.79 +0.1 222,521 + IAI47_19965 0.79 -0.4 222,522 - IAI47_19965 0.80 +0.2 222,522 - IAI47_19965 0.80 -0.8 222,522 - IAI47_19965 0.80 -1.2 222,522 - IAI47_19965 0.80 -0.9 222,522 - IAI47_19965 0.80 -1.8 222,522 - IAI47_19965 0.80 +2.0 222,522 - IAI47_19965 0.80 -1.2 222,522 - IAI47_19965 0.80 -2.0 222,617 + IAI47_19965 0.84 +0.1 222,617 + IAI47_19965 0.84 -0.4 222,618 - IAI47_19965 0.84 -1.4 222,677 - IAI47_19965 0.87 -0.4 222,688 + IAI47_19965 0.87 +2.0 222,688 + IAI47_19965 0.87 -0.1 222,688 + IAI47_19965 0.87 -2.5 222,689 - IAI47_19965 0.87 -1.8 222,689 - IAI47_19965 0.87 -0.5 222,689 - IAI47_19965 0.87 -0.6 222,689 - IAI47_19965 0.87 +0.8 222,689 - IAI47_19965 0.87 -0.2 222,692 + IAI47_19965 0.87 +1.2 222,693 - IAI47_19965 0.87 +1.2 222,693 - -1.4 222,812 + +1.2 222,812 + -0.8 222,812 + +2.0 222,812 + -2.8 222,812 + +0.6 222,812 + -1.9 222,813 - +0.2 222,813 - -0.5 222,813 - -0.4 222,813 - -1.1 222,813 - +0.4 222,813 - -1.3 222,813 - -0.4 222,828 + -3.3 222,828 + +2.0 222,880 + +1.6 222,880 + -1.4 222,881 - +1.0 222,899 + +3.5 222,899 + -1.4 222,930 - +1.2 222,966 - +0.9 222,966 - +0.2 222,981 + +1.2 222,981 + -1.8 222,981 + -1.9 222,982 - -1.4 222,989 + -1.0 222,990 - +2.0 222,990 - -1.1 223,033 + +0.3 223,033 + -1.4 223,033 + +1.5 223,033 + +0.1 223,033 + -2.2 223,033 + -1.8 223,033 + -1.6 223,034 - +0.9 223,034 - -1.1 223,034 - -2.4 223,034 - +0.5 223,034 - +0.3 223,034 - -1.6 223,052 + -1.8 223,052 + -2.1 223,053 - -1.1 223,053 - +0.5 223,060 - -3.8 223,203 + -3.0 223,204 - -1.8 223,204 - -0.7 223,204 - +2.1 223,314 + IAI47_19970 0.14 -1.9 223,314 + IAI47_19970 0.14 -0.4 223,314 + IAI47_19970 0.14 -0.5 223,315 - IAI47_19970 0.14 +0.2 223,315 - IAI47_19970 0.14 -0.5 223,315 - IAI47_19970 0.14 +1.6 223,315 - IAI47_19970 0.14 -2.1 223,368 + IAI47_19970 0.19 +0.8 223,369 - IAI47_19970 0.19 -2.0 223,369 - IAI47_19970 0.19 +1.2 223,417 + IAI47_19970 0.23 -0.2 223,417 + IAI47_19970 0.23 -0.6 223,418 - IAI47_19970 0.23 +1.6 223,418 - IAI47_19970 0.23 +2.2 223,420 + IAI47_19970 0.23 -1.0 223,420 + IAI47_19970 0.23 -1.2 223,421 - IAI47_19970 0.23 -1.6 223,483 + IAI47_19970 0.29 +0.5 223,548 - IAI47_19970 0.35 -0.4 223,548 - IAI47_19970 0.35 -1.6 223,591 + IAI47_19970 0.38 -2.2 223,592 - IAI47_19970 0.38 -0.9 223,592 - IAI47_19970 0.38 -3.9 223,646 + IAI47_19970 0.43 +1.4 223,681 + IAI47_19970 0.46 -0.5 223,681 + IAI47_19970 0.46 +0.8 223,682 - IAI47_19970 0.46 -0.3 223,682 - IAI47_19970 0.46 -0.4 223,779 + IAI47_19970 0.55 -0.7 223,780 - IAI47_19970 0.55 -0.1 223,780 - IAI47_19970 0.55 -1.4 224,214 + +0.8 224,214 + -3.9 224,214 + +1.1 224,214 + +0.2 224,214 + -1.8 224,215 - -1.3 224,215 - -0.6 224,215 - +1.8 224,215 - +1.0 224,215 - -2.2 224,279 + -2.2 224,279 + -0.8 224,279 + -0.5 224,296 + -1.6 224,297 - -0.6 224,297 - +0.4 224,297 - +1.0 224,315 + +0.5 224,316 - +0.1 224,324 + -1.8 224,375 + -0.1 224,375 + -2.1 224,375 + +0.9 224,375 + -0.6 224,376 - -3.2 224,399 + +1.0 224,400 - -1.9 224,407 + -1.0 224,435 + +0.6 224,435 + +0.6 224,435 + -1.4 224,435 + -2.5 224,435 + -1.9 224,436 - +1.5 224,504 + -0.4 224,504 + +1.2 224,504 + +0.1 224,505 - -1.6 224,512 - +1.2 224,594 + +1.8 224,594 + -1.4 224,594 + +0.8 224,595 - -1.9 224,599 + -0.2 224,600 - -1.1 224,625 + +0.7 224,626 - -0.4 224,736 + IAI47_19975 0.18 +1.2 224,802 + IAI47_19975 0.24 -0.8 224,802 + IAI47_19975 0.24 +0.1 224,802 + IAI47_19975 0.24 +0.8 224,802 + IAI47_19975 0.24 +0.4 224,802 + IAI47_19975 0.24 -1.9 224,802 + IAI47_19975 0.24 +2.0 224,802 + IAI47_19975 0.24 +3.5 224,802 + IAI47_19975 0.24 +0.6 224,802 + IAI47_19975 0.24 +1.2 224,802 + IAI47_19975 0.24 +0.2 224,803 - IAI47_19975 0.24 +2.3 224,803 - IAI47_19975 0.24 +0.2 224,803 - IAI47_19975 0.24 +1.2 224,803 - IAI47_19975 0.24 +0.1 224,803 - IAI47_19975 0.24 +1.2 224,803 - IAI47_19975 0.24 -0.4 224,876 + IAI47_19975 0.30 +0.2 224,876 + IAI47_19975 0.30 -2.6 224,877 - IAI47_19975 0.30 -1.4 224,877 - IAI47_19975 0.30 -0.1 224,877 - IAI47_19975 0.30 -2.5 224,877 - IAI47_19975 0.30 +1.6 224,906 + IAI47_19975 0.32 +1.2 224,927 - IAI47_19975 0.34 +0.4 224,927 - IAI47_19975 0.34 +0.9 224,927 - IAI47_19975 0.34 -0.2 224,960 + IAI47_19975 0.37 +1.2 224,960 + IAI47_19975 0.37 -2.7 224,961 - IAI47_19975 0.37 -0.2 224,961 - IAI47_19975 0.37 +0.1 224,961 - IAI47_19975 0.37 -0.3 224,961 - IAI47_19975 0.37 -0.1 224,961 - IAI47_19975 0.37 -0.8 224,961 - IAI47_19975 0.37 +1.5 224,970 + IAI47_19975 0.37 -0.4 224,970 + IAI47_19975 0.37 -1.4 224,971 - IAI47_19975 0.38 -0.9 224,971 - IAI47_19975 0.38 -0.4 224,971 - IAI47_19975 0.38 -1.9 224,971 - IAI47_19975 0.38 +1.3 225,024 - IAI47_19975 0.42 -0.4 225,024 - IAI47_19975 0.42 +0.2 225,098 + IAI47_19975 0.48 -1.1 225,099 - IAI47_19975 0.48 -0.9 225,106 - IAI47_19975 0.49 -0.8 225,171 + IAI47_19975 0.54 -1.6 225,171 + IAI47_19975 0.54 +0.1 225,171 + IAI47_19975 0.54 -0.4 225,171 + IAI47_19975 0.54 +1.2 225,171 + IAI47_19975 0.54 +1.9 225,171 + IAI47_19975 0.54 -0.8 225,171 + IAI47_19975 0.54 +1.3 225,172 - IAI47_19975 0.54 -0.5 225,172 - IAI47_19975 0.54 -0.4 225,172 - IAI47_19975 0.54 -0.3 225,172 - IAI47_19975 0.54 -0.4 225,172 - IAI47_19975 0.54 -2.8 225,172 - IAI47_19975 0.54 -0.4 225,176 + IAI47_19975 0.54 +1.8 225,176 + IAI47_19975 0.54 +0.8 225,177 - IAI47_19975 0.54 +1.2 225,177 - IAI47_19975 0.54 +0.2 225,177 - IAI47_19975 0.54 +1.2
Or see this region's nucleotide sequence