Experiment: Survival; Treatment=UGA synthetic gw pH7.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_01705 and IAI47_01710 overlap by 20 nucleotides IAI47_01710 and IAI47_01715 overlap by 4 nucleotides IAI47_01715 and IAI47_01720 are separated by 1 nucleotides
IAI47_01705: IAI47_01705 - LysR family transcriptional regulator, at 374,001 to 374,936
_01705
IAI47_01710: IAI47_01710 - malonate decarboxylase subunit epsilon, at 374,917 to 375,819
_01710
IAI47_01715: IAI47_01715 - malonate decarboxylase holo-ACP synthase, at 375,816 to 376,433
_01715
IAI47_01720: IAI47_01720 - AEC family transporter, at 376,435 to 377,394
_01720
Position (kb) 375
376
377 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 374.844 kb on + strand at 374.844 kb on + strand at 374.918 kb on + strand at 374.918 kb on + strand at 374.919 kb on - strand at 374.919 kb on - strand at 374.919 kb on - strand at 374.926 kb on + strand at 374.926 kb on + strand at 374.926 kb on + strand at 374.926 kb on + strand at 374.964 kb on + strand at 374.964 kb on + strand at 375.111 kb on + strand, within IAI47_01710 at 375.112 kb on - strand, within IAI47_01710 at 375.112 kb on - strand, within IAI47_01710 at 375.112 kb on - strand, within IAI47_01710 at 375.112 kb on - strand, within IAI47_01710 at 375.114 kb on + strand, within IAI47_01710 at 375.114 kb on + strand, within IAI47_01710 at 375.179 kb on + strand, within IAI47_01710 at 375.179 kb on + strand, within IAI47_01710 at 375.179 kb on + strand, within IAI47_01710 at 375.180 kb on - strand, within IAI47_01710 at 375.180 kb on - strand, within IAI47_01710 at 375.243 kb on + strand, within IAI47_01710 at 375.285 kb on + strand, within IAI47_01710 at 375.286 kb on - strand, within IAI47_01710 at 375.286 kb on - strand, within IAI47_01710 at 375.286 kb on - strand, within IAI47_01710 at 375.294 kb on + strand, within IAI47_01710 at 375.392 kb on + strand, within IAI47_01710 at 375.392 kb on + strand, within IAI47_01710 at 375.392 kb on + strand, within IAI47_01710 at 375.392 kb on + strand, within IAI47_01710 at 375.392 kb on + strand, within IAI47_01710 at 375.392 kb on + strand, within IAI47_01710 at 375.392 kb on + strand, within IAI47_01710 at 375.392 kb on + strand, within IAI47_01710 at 375.392 kb on + strand, within IAI47_01710 at 375.393 kb on - strand, within IAI47_01710 at 375.393 kb on - strand, within IAI47_01710 at 375.393 kb on - strand, within IAI47_01710 at 375.393 kb on - strand, within IAI47_01710 at 375.393 kb on - strand, within IAI47_01710 at 375.393 kb on - strand, within IAI47_01710 at 375.393 kb on - strand, within IAI47_01710 at 375.393 kb on - strand, within IAI47_01710 at 375.399 kb on + strand, within IAI47_01710 at 375.399 kb on + strand, within IAI47_01710 at 375.436 kb on + strand, within IAI47_01710 at 375.461 kb on + strand, within IAI47_01710 at 375.461 kb on + strand, within IAI47_01710 at 375.461 kb on + strand, within IAI47_01710 at 375.462 kb on - strand, within IAI47_01710 at 375.462 kb on - strand, within IAI47_01710 at 375.804 kb on + strand at 375.804 kb on + strand at 375.804 kb on + strand at 375.804 kb on + strand at 375.804 kb on + strand at 375.804 kb on + strand at 375.804 kb on + strand at 375.804 kb on + strand at 375.805 kb on - strand at 375.805 kb on - strand at 375.811 kb on + strand at 375.811 kb on + strand at 375.811 kb on + strand at 375.812 kb on - strand at 375.941 kb on + strand, within IAI47_01715 at 375.942 kb on - strand, within IAI47_01715 at 375.942 kb on - strand, within IAI47_01715 at 376.078 kb on + strand, within IAI47_01715 at 376.079 kb on - strand, within IAI47_01715 at 376.157 kb on - strand, within IAI47_01715 at 376.157 kb on - strand, within IAI47_01715 at 376.157 kb on - strand, within IAI47_01715 at 376.224 kb on - strand, within IAI47_01715 at 376.226 kb on + strand, within IAI47_01715 at 376.226 kb on + strand, within IAI47_01715 at 376.226 kb on + strand, within IAI47_01715 at 376.226 kb on + strand, within IAI47_01715 at 376.226 kb on + strand, within IAI47_01715 at 376.226 kb on + strand, within IAI47_01715 at 376.226 kb on + strand, within IAI47_01715 at 376.226 kb on + strand, within IAI47_01715 at 376.226 kb on + strand, within IAI47_01715 at 376.226 kb on + strand, within IAI47_01715 at 376.226 kb on + strand, within IAI47_01715 at 376.226 kb on + strand, within IAI47_01715 at 376.227 kb on - strand, within IAI47_01715 at 376.227 kb on - strand, within IAI47_01715 at 376.227 kb on - strand, within IAI47_01715 at 376.227 kb on - strand, within IAI47_01715 at 376.227 kb on - strand, within IAI47_01715 at 376.380 kb on + strand at 376.380 kb on + strand at 376.380 kb on + strand at 376.433 kb on + strand at 376.433 kb on + strand at 376.433 kb on + strand at 376.433 kb on + strand at 376.433 kb on + strand at 376.433 kb on + strand at 376.433 kb on + strand at 376.434 kb on - strand at 376.434 kb on - strand at 376.434 kb on - strand at 376.434 kb on - strand at 376.434 kb on - strand at 376.434 kb on - strand at 376.434 kb on - strand at 376.436 kb on + strand at 376.436 kb on + strand at 376.436 kb on + strand at 376.436 kb on + strand at 376.436 kb on + strand at 376.436 kb on + strand at 376.436 kb on + strand at 376.436 kb on + strand at 376.437 kb on - strand at 376.437 kb on - strand at 376.437 kb on - strand at 376.437 kb on - strand at 376.437 kb on - strand at 376.437 kb on - strand at 376.437 kb on - strand at 376.437 kb on - strand at 376.437 kb on - strand at 376.455 kb on + strand at 376.455 kb on + strand at 376.455 kb on + strand at 376.455 kb on + strand at 376.455 kb on + strand at 376.455 kb on + strand at 376.468 kb on + strand at 376.474 kb on + strand at 376.474 kb on + strand at 376.475 kb on - strand at 376.480 kb on - strand at 376.480 kb on - strand at 376.495 kb on + strand at 376.496 kb on - strand at 376.496 kb on - strand at 376.496 kb on - strand at 376.496 kb on - strand at 376.579 kb on + strand, within IAI47_01720 at 376.579 kb on + strand, within IAI47_01720 at 376.580 kb on - strand, within IAI47_01720 at 376.580 kb on - strand, within IAI47_01720 at 376.591 kb on - strand, within IAI47_01720 at 376.591 kb on - strand, within IAI47_01720 at 376.777 kb on - strand, within IAI47_01720 at 377.035 kb on + strand, within IAI47_01720 at 377.035 kb on + strand, within IAI47_01720 at 377.036 kb on - strand, within IAI47_01720 at 377.069 kb on + strand, within IAI47_01720 at 377.095 kb on + strand, within IAI47_01720 at 377.095 kb on + strand, within IAI47_01720 at 377.095 kb on + strand, within IAI47_01720 at 377.098 kb on + strand, within IAI47_01720 at 377.098 kb on + strand, within IAI47_01720 at 377.099 kb on - strand, within IAI47_01720 at 377.124 kb on + strand, within IAI47_01720 at 377.124 kb on + strand, within IAI47_01720 at 377.147 kb on + strand, within IAI47_01720 at 377.148 kb on - strand, within IAI47_01720 at 377.148 kb on - strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.159 kb on + strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.160 kb on - strand, within IAI47_01720 at 377.165 kb on - strand, within IAI47_01720 at 377.183 kb on - strand, within IAI47_01720 at 377.284 kb on - strand, within IAI47_01720 at 377.307 kb on + strand at 377.325 kb on + strand at 377.341 kb on + strand at 377.341 kb on + strand at 377.342 kb on - strand at 377.342 kb on - strand at 377.349 kb on + strand at 377.349 kb on + strand at 377.349 kb on + strand at 377.349 kb on + strand at 377.349 kb on + strand at 377.349 kb on + strand at 377.349 kb on + strand at 377.350 kb on - strand at 377.350 kb on - strand at 377.350 kb on - strand at 377.350 kb on - strand at 377.350 kb on - strand at 377.351 kb on - strand at 377.355 kb on + strand at 377.356 kb on - strand at 377.383 kb on + strand at 377.383 kb on + strand at 377.383 kb on + strand at 377.383 kb on + strand at 377.383 kb on + strand at 377.384 kb on - strand at 377.384 kb on - strand at 377.384 kb on - strand at 377.384 kb on - strand at 377.384 kb on - strand at 377.387 kb on + strand at 377.387 kb on + strand at 377.388 kb on - strand at 377.388 kb on - strand at 377.388 kb on - strand at 377.394 kb on + strand at 377.395 kb on - strand at 377.395 kb on - strand at 377.405 kb on + strand at 377.405 kb on + strand at 377.405 kb on + strand at 377.410 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 374,844 + -0.6 374,844 + -0.7 374,918 + +0.1 374,918 + -0.4 374,919 - +1.3 374,919 - +0.6 374,919 - -0.1 374,926 + -0.8 374,926 + -1.6 374,926 + -0.1 374,926 + +3.8 374,964 + +0.2 374,964 + +1.5 375,111 + IAI47_01710 0.21 -0.2 375,112 - IAI47_01710 0.22 -1.6 375,112 - IAI47_01710 0.22 -0.1 375,112 - IAI47_01710 0.22 +5.3 375,112 - IAI47_01710 0.22 -2.4 375,114 + IAI47_01710 0.22 +1.6 375,114 + IAI47_01710 0.22 -0.3 375,179 + IAI47_01710 0.29 -1.2 375,179 + IAI47_01710 0.29 -0.4 375,179 + IAI47_01710 0.29 +3.2 375,180 - IAI47_01710 0.29 -0.9 375,180 - IAI47_01710 0.29 +1.8 375,243 + IAI47_01710 0.36 +0.4 375,285 + IAI47_01710 0.41 -2.4 375,286 - IAI47_01710 0.41 +0.8 375,286 - IAI47_01710 0.41 -1.1 375,286 - IAI47_01710 0.41 -3.2 375,294 + IAI47_01710 0.42 +4.3 375,392 + IAI47_01710 0.53 +2.1 375,392 + IAI47_01710 0.53 +0.2 375,392 + IAI47_01710 0.53 +0.3 375,392 + IAI47_01710 0.53 -1.7 375,392 + IAI47_01710 0.53 +0.3 375,392 + IAI47_01710 0.53 +0.9 375,392 + IAI47_01710 0.53 +1.5 375,392 + IAI47_01710 0.53 +0.1 375,392 + IAI47_01710 0.53 +1.5 375,393 - IAI47_01710 0.53 -0.0 375,393 - IAI47_01710 0.53 +0.7 375,393 - IAI47_01710 0.53 +2.6 375,393 - IAI47_01710 0.53 -1.7 375,393 - IAI47_01710 0.53 +0.2 375,393 - IAI47_01710 0.53 +1.1 375,393 - IAI47_01710 0.53 -0.9 375,393 - IAI47_01710 0.53 -1.0 375,399 + IAI47_01710 0.53 -0.4 375,399 + IAI47_01710 0.53 -1.6 375,436 + IAI47_01710 0.57 -1.3 375,461 + IAI47_01710 0.60 +0.4 375,461 + IAI47_01710 0.60 +1.0 375,461 + IAI47_01710 0.60 +3.0 375,462 - IAI47_01710 0.60 -0.4 375,462 - IAI47_01710 0.60 -2.1 375,804 + -0.5 375,804 + +2.3 375,804 + +0.6 375,804 + +0.6 375,804 + -1.5 375,804 + +0.1 375,804 + +1.0 375,804 + -0.5 375,805 - -2.4 375,805 - +0.4 375,811 + +0.7 375,811 + -0.6 375,811 + -0.7 375,812 - +1.8 375,941 + IAI47_01715 0.20 +1.1 375,942 - IAI47_01715 0.20 -0.8 375,942 - IAI47_01715 0.20 +0.8 376,078 + IAI47_01715 0.42 +1.1 376,079 - IAI47_01715 0.43 +1.6 376,157 - IAI47_01715 0.55 +1.4 376,157 - IAI47_01715 0.55 +0.6 376,157 - IAI47_01715 0.55 +2.0 376,224 - IAI47_01715 0.66 -0.6 376,226 + IAI47_01715 0.66 +1.1 376,226 + IAI47_01715 0.66 +3.6 376,226 + IAI47_01715 0.66 +2.6 376,226 + IAI47_01715 0.66 +0.2 376,226 + IAI47_01715 0.66 -2.8 376,226 + IAI47_01715 0.66 +1.0 376,226 + IAI47_01715 0.66 +1.1 376,226 + IAI47_01715 0.66 -0.2 376,226 + IAI47_01715 0.66 -1.2 376,226 + IAI47_01715 0.66 -1.9 376,226 + IAI47_01715 0.66 -2.8 376,226 + IAI47_01715 0.66 -0.7 376,227 - IAI47_01715 0.67 -1.3 376,227 - IAI47_01715 0.67 +1.4 376,227 - IAI47_01715 0.67 +1.0 376,227 - IAI47_01715 0.67 -0.5 376,227 - IAI47_01715 0.67 +2.4 376,380 + +1.0 376,380 + +1.1 376,380 + -0.8 376,433 + -0.8 376,433 + -1.0 376,433 + +1.8 376,433 + -0.7 376,433 + -0.3 376,433 + +1.0 376,433 + -2.6 376,434 - -0.8 376,434 - +0.6 376,434 - +0.3 376,434 - +0.6 376,434 - -1.0 376,434 - +0.1 376,434 - -1.5 376,436 + +0.1 376,436 + +0.4 376,436 + +2.4 376,436 + -0.3 376,436 + -0.5 376,436 + -1.0 376,436 + +1.5 376,436 + -0.7 376,437 - -0.1 376,437 - -1.2 376,437 - +0.6 376,437 - -2.1 376,437 - +1.1 376,437 - -0.1 376,437 - +1.4 376,437 - +0.4 376,437 - -0.8 376,455 + -0.0 376,455 + -0.3 376,455 + +2.5 376,455 + -0.4 376,455 + +1.8 376,455 + +1.4 376,468 + +1.3 376,474 + +1.4 376,474 + +0.2 376,475 - +0.7 376,480 - -1.9 376,480 - +1.6 376,495 + -0.4 376,496 - -0.9 376,496 - -0.8 376,496 - +2.8 376,496 - -2.4 376,579 + IAI47_01720 0.15 +0.8 376,579 + IAI47_01720 0.15 +0.3 376,580 - IAI47_01720 0.15 -0.9 376,580 - IAI47_01720 0.15 -0.2 376,591 - IAI47_01720 0.16 -1.9 376,591 - IAI47_01720 0.16 +1.6 376,777 - IAI47_01720 0.36 +1.3 377,035 + IAI47_01720 0.62 +0.7 377,035 + IAI47_01720 0.62 -2.4 377,036 - IAI47_01720 0.63 +0.3 377,069 + IAI47_01720 0.66 -0.9 377,095 + IAI47_01720 0.69 +2.5 377,095 + IAI47_01720 0.69 +2.4 377,095 + IAI47_01720 0.69 -1.0 377,098 + IAI47_01720 0.69 -1.5 377,098 + IAI47_01720 0.69 +2.6 377,099 - IAI47_01720 0.69 -0.6 377,124 + IAI47_01720 0.72 -0.9 377,124 + IAI47_01720 0.72 -2.0 377,147 + IAI47_01720 0.74 +0.9 377,148 - IAI47_01720 0.74 -2.9 377,148 - IAI47_01720 0.74 -1.3 377,159 + IAI47_01720 0.75 +0.8 377,159 + IAI47_01720 0.75 +2.5 377,159 + IAI47_01720 0.75 +1.8 377,159 + IAI47_01720 0.75 +0.1 377,159 + IAI47_01720 0.75 +0.8 377,159 + IAI47_01720 0.75 +0.8 377,159 + IAI47_01720 0.75 +0.1 377,159 + IAI47_01720 0.75 +2.8 377,159 + IAI47_01720 0.75 -2.7 377,159 + IAI47_01720 0.75 +0.8 377,159 + IAI47_01720 0.75 +1.5 377,159 + IAI47_01720 0.75 -1.6 377,159 + IAI47_01720 0.75 -0.8 377,159 + IAI47_01720 0.75 +0.8 377,159 + IAI47_01720 0.75 +1.7 377,159 + IAI47_01720 0.75 -0.6 377,159 + IAI47_01720 0.75 -0.5 377,159 + IAI47_01720 0.75 -1.9 377,160 - IAI47_01720 0.76 -2.3 377,160 - IAI47_01720 0.76 -0.1 377,160 - IAI47_01720 0.76 -0.2 377,160 - IAI47_01720 0.76 -1.3 377,160 - IAI47_01720 0.76 -0.8 377,160 - IAI47_01720 0.76 +1.2 377,160 - IAI47_01720 0.76 -1.6 377,160 - IAI47_01720 0.76 -1.3 377,160 - IAI47_01720 0.76 +0.1 377,160 - IAI47_01720 0.76 +1.6 377,160 - IAI47_01720 0.76 -1.4 377,160 - IAI47_01720 0.76 -0.2 377,160 - IAI47_01720 0.76 +0.6 377,160 - IAI47_01720 0.76 -0.8 377,160 - IAI47_01720 0.76 +1.1 377,160 - IAI47_01720 0.76 +0.1 377,160 - IAI47_01720 0.76 -0.7 377,160 - IAI47_01720 0.76 +0.6 377,165 - IAI47_01720 0.76 +0.2 377,183 - IAI47_01720 0.78 -2.0 377,284 - IAI47_01720 0.88 +2.7 377,307 + -0.4 377,325 + -0.0 377,341 + -1.1 377,341 + +1.7 377,342 - +0.7 377,342 - -0.6 377,349 + -0.8 377,349 + +0.4 377,349 + -1.5 377,349 + -0.5 377,349 + +0.2 377,349 + -0.9 377,349 + +0.4 377,350 - +3.9 377,350 - -1.7 377,350 - -3.7 377,350 - +2.5 377,350 - +3.2 377,351 - +0.9 377,355 + +0.3 377,356 - -0.4 377,383 + -1.1 377,383 + -2.3 377,383 + +0.3 377,383 + -1.7 377,383 + -0.7 377,384 - +0.2 377,384 - -1.0 377,384 - -0.3 377,384 - -1.3 377,384 - -2.8 377,387 + -1.0 377,387 + +1.0 377,388 - +2.4 377,388 - +0.8 377,388 - +0.8 377,394 + +0.7 377,395 - -2.3 377,395 - +0.0 377,405 + -0.9 377,405 + -0.5 377,405 + +0.9 377,410 - +1.6
Or see this region's nucleotide sequence