Experiment: Survival; Treatment=UGA synthetic gw pH7.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_00650 and IAI47_00655 are separated by 92 nucleotides IAI47_00655 and IAI47_00660 overlap by 8 nucleotides
IAI47_00650: IAI47_00650 - lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase, at 139,583 to 140,857
_00650
IAI47_00655: IAI47_00655 - glycosyltransferase family 4 protein, at 140,950 to 142,083
_00655
IAI47_00660: IAI47_00660 - putative lipopolysaccharide heptosyltransferase III, at 142,076 to 143,152
_00660
Position (kb)
140
141
142
143 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 140.858 kb on - strand at 140.858 kb on - strand at 140.858 kb on - strand at 140.858 kb on - strand at 140.858 kb on - strand at 140.858 kb on - strand at 140.858 kb on - strand at 140.862 kb on - strand at 140.862 kb on - strand at 140.862 kb on - strand at 140.868 kb on - strand at 140.868 kb on - strand at 140.896 kb on - strand at 140.896 kb on - strand at 140.917 kb on - strand at 140.917 kb on - strand at 140.958 kb on + strand at 140.959 kb on - strand at 140.965 kb on + strand at 140.965 kb on + strand at 140.965 kb on + strand at 140.966 kb on - strand at 140.966 kb on - strand at 141.240 kb on + strand, within IAI47_00655 at 141.273 kb on - strand, within IAI47_00655 at 141.280 kb on + strand, within IAI47_00655 at 141.325 kb on - strand, within IAI47_00655 at 141.325 kb on - strand, within IAI47_00655 at 141.464 kb on - strand, within IAI47_00655 at 141.695 kb on + strand, within IAI47_00655 at 141.703 kb on - strand, within IAI47_00655 at 142.073 kb on - strand at 142.090 kb on - strand at 142.138 kb on - strand at 142.138 kb on - strand at 142.138 kb on - strand at 142.138 kb on - strand at 142.162 kb on + strand at 142.162 kb on + strand at 142.163 kb on - strand at 142.204 kb on + strand, within IAI47_00660 at 142.205 kb on - strand, within IAI47_00660 at 142.205 kb on - strand, within IAI47_00660 at 142.267 kb on + strand, within IAI47_00660 at 142.267 kb on + strand, within IAI47_00660 at 142.267 kb on + strand, within IAI47_00660 at 142.267 kb on + strand, within IAI47_00660 at 142.268 kb on - strand, within IAI47_00660 at 142.268 kb on - strand, within IAI47_00660 at 142.322 kb on - strand, within IAI47_00660 at 142.322 kb on - strand, within IAI47_00660 at 142.418 kb on - strand, within IAI47_00660 at 142.418 kb on - strand, within IAI47_00660 at 142.445 kb on - strand, within IAI47_00660 at 142.459 kb on + strand, within IAI47_00660 at 142.459 kb on + strand, within IAI47_00660 at 142.459 kb on + strand, within IAI47_00660 at 142.459 kb on + strand, within IAI47_00660 at 142.460 kb on - strand, within IAI47_00660 at 142.460 kb on - strand, within IAI47_00660 at 142.460 kb on - strand, within IAI47_00660 at 142.531 kb on + strand, within IAI47_00660 at 142.532 kb on - strand, within IAI47_00660 at 142.532 kb on - strand, within IAI47_00660 at 142.532 kb on - strand, within IAI47_00660 at 142.532 kb on - strand, within IAI47_00660 at 142.532 kb on - strand, within IAI47_00660 at 142.532 kb on - strand, within IAI47_00660 at 142.532 kb on - strand, within IAI47_00660 at 142.564 kb on + strand, within IAI47_00660 at 142.565 kb on - strand, within IAI47_00660 at 142.565 kb on - strand, within IAI47_00660 at 142.565 kb on - strand, within IAI47_00660 at 142.565 kb on - strand, within IAI47_00660 at 142.565 kb on - strand, within IAI47_00660 at 142.565 kb on - strand, within IAI47_00660 at 142.565 kb on - strand, within IAI47_00660 at 142.565 kb on - strand, within IAI47_00660 at 142.566 kb on + strand, within IAI47_00660 at 142.566 kb on + strand, within IAI47_00660 at 142.567 kb on - strand, within IAI47_00660 at 142.567 kb on - strand, within IAI47_00660 at 142.567 kb on - strand, within IAI47_00660 at 142.567 kb on - strand, within IAI47_00660 at 142.567 kb on - strand, within IAI47_00660 at 142.567 kb on - strand, within IAI47_00660 at 142.567 kb on - strand, within IAI47_00660 at 142.567 kb on - strand, within IAI47_00660 at 142.567 kb on - strand, within IAI47_00660 at 142.567 kb on - strand, within IAI47_00660 at 142.567 kb on - strand, within IAI47_00660 at 142.567 kb on - strand, within IAI47_00660 at 142.567 kb on - strand, within IAI47_00660 at 142.567 kb on - strand, within IAI47_00660 at 142.629 kb on + strand, within IAI47_00660 at 142.629 kb on + strand, within IAI47_00660 at 142.629 kb on + strand, within IAI47_00660 at 142.630 kb on - strand, within IAI47_00660 at 142.630 kb on - strand, within IAI47_00660 at 142.652 kb on - strand, within IAI47_00660 at 142.661 kb on - strand, within IAI47_00660 at 142.661 kb on - strand, within IAI47_00660 at 142.687 kb on + strand, within IAI47_00660 at 142.687 kb on + strand, within IAI47_00660 at 142.687 kb on + strand, within IAI47_00660 at 142.687 kb on + strand, within IAI47_00660 at 142.687 kb on + strand, within IAI47_00660 at 142.687 kb on + strand, within IAI47_00660 at 142.688 kb on - strand, within IAI47_00660 at 142.688 kb on - strand, within IAI47_00660 at 142.688 kb on - strand, within IAI47_00660 at 142.688 kb on - strand, within IAI47_00660 at 142.688 kb on - strand, within IAI47_00660 at 142.688 kb on - strand, within IAI47_00660 at 142.688 kb on - strand, within IAI47_00660 at 142.688 kb on - strand, within IAI47_00660 at 142.688 kb on - strand, within IAI47_00660 at 142.688 kb on - strand, within IAI47_00660 at 142.688 kb on - strand, within IAI47_00660 at 142.703 kb on - strand, within IAI47_00660 at 142.703 kb on - strand, within IAI47_00660 at 142.720 kb on + strand, within IAI47_00660 at 142.721 kb on - strand, within IAI47_00660 at 142.721 kb on - strand, within IAI47_00660 at 142.721 kb on - strand, within IAI47_00660 at 142.721 kb on - strand, within IAI47_00660 at 142.721 kb on - strand, within IAI47_00660 at 142.721 kb on - strand, within IAI47_00660 at 142.721 kb on - strand, within IAI47_00660 at 142.721 kb on - strand, within IAI47_00660 at 142.721 kb on - strand, within IAI47_00660 at 142.721 kb on - strand, within IAI47_00660 at 142.721 kb on - strand, within IAI47_00660 at 142.721 kb on - strand, within IAI47_00660 at 142.721 kb on - strand, within IAI47_00660 at 142.741 kb on + strand, within IAI47_00660 at 142.742 kb on - strand, within IAI47_00660 at 142.779 kb on + strand, within IAI47_00660 at 142.854 kb on - strand, within IAI47_00660 at 142.854 kb on - strand, within IAI47_00660 at 142.866 kb on + strand, within IAI47_00660 at 142.867 kb on - strand, within IAI47_00660 at 142.867 kb on - strand, within IAI47_00660 at 142.867 kb on - strand, within IAI47_00660 at 142.867 kb on - strand, within IAI47_00660 at 142.867 kb on - strand, within IAI47_00660 at 142.893 kb on - strand, within IAI47_00660 at 142.916 kb on - strand, within IAI47_00660 at 143.005 kb on + strand, within IAI47_00660 at 143.005 kb on + strand, within IAI47_00660 at 143.006 kb on - strand, within IAI47_00660 at 143.007 kb on + strand, within IAI47_00660 at 143.007 kb on + strand, within IAI47_00660 at 143.008 kb on - strand, within IAI47_00660
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=UGA synthetic gw pH7.4; TreatmentDuration=0.5hrs; Outgrowth=LBkan50 remove 140,858 - -2.9 140,858 - +0.3 140,858 - -2.0 140,858 - -1.2 140,858 - -1.3 140,858 - +1.4 140,858 - -1.8 140,862 - -0.3 140,862 - -1.6 140,862 - -0.8 140,868 - +1.5 140,868 - +0.2 140,896 - +0.8 140,896 - +0.7 140,917 - +1.9 140,917 - +0.1 140,958 + +0.5 140,959 - -0.1 140,965 + -2.2 140,965 + -1.7 140,965 + +0.4 140,966 - -0.6 140,966 - +0.1 141,240 + IAI47_00655 0.26 -0.3 141,273 - IAI47_00655 0.28 -0.4 141,280 + IAI47_00655 0.29 -1.0 141,325 - IAI47_00655 0.33 -1.0 141,325 - IAI47_00655 0.33 -0.7 141,464 - IAI47_00655 0.45 -2.3 141,695 + IAI47_00655 0.66 -1.0 141,703 - IAI47_00655 0.66 -2.3 142,073 - +1.9 142,090 - -0.5 142,138 - -0.3 142,138 - +1.1 142,138 - +0.8 142,138 - -2.1 142,162 + +0.5 142,162 + -0.7 142,163 - +0.4 142,204 + IAI47_00660 0.12 -0.1 142,205 - IAI47_00660 0.12 -0.8 142,205 - IAI47_00660 0.12 -0.8 142,267 + IAI47_00660 0.18 +0.1 142,267 + IAI47_00660 0.18 -1.0 142,267 + IAI47_00660 0.18 -3.1 142,267 + IAI47_00660 0.18 +0.7 142,268 - IAI47_00660 0.18 +0.6 142,268 - IAI47_00660 0.18 +0.6 142,322 - IAI47_00660 0.23 -1.2 142,322 - IAI47_00660 0.23 +1.7 142,418 - IAI47_00660 0.32 -0.1 142,418 - IAI47_00660 0.32 +0.6 142,445 - IAI47_00660 0.34 +1.2 142,459 + IAI47_00660 0.36 +1.8 142,459 + IAI47_00660 0.36 -0.0 142,459 + IAI47_00660 0.36 +0.1 142,459 + IAI47_00660 0.36 +1.8 142,460 - IAI47_00660 0.36 +1.1 142,460 - IAI47_00660 0.36 +0.4 142,460 - IAI47_00660 0.36 +2.8 142,531 + IAI47_00660 0.42 +1.7 142,532 - IAI47_00660 0.42 +0.1 142,532 - IAI47_00660 0.42 +1.2 142,532 - IAI47_00660 0.42 -0.5 142,532 - IAI47_00660 0.42 +2.7 142,532 - IAI47_00660 0.42 -1.5 142,532 - IAI47_00660 0.42 +2.1 142,532 - IAI47_00660 0.42 -0.2 142,564 + IAI47_00660 0.45 -0.1 142,565 - IAI47_00660 0.45 +0.1 142,565 - IAI47_00660 0.45 -0.7 142,565 - IAI47_00660 0.45 -0.3 142,565 - IAI47_00660 0.45 +0.2 142,565 - IAI47_00660 0.45 -0.0 142,565 - IAI47_00660 0.45 +3.0 142,565 - IAI47_00660 0.45 +1.3 142,565 - IAI47_00660 0.45 +2.3 142,566 + IAI47_00660 0.45 +0.6 142,566 + IAI47_00660 0.45 -2.0 142,567 - IAI47_00660 0.46 +0.7 142,567 - IAI47_00660 0.46 -0.8 142,567 - IAI47_00660 0.46 +0.8 142,567 - IAI47_00660 0.46 +2.2 142,567 - IAI47_00660 0.46 -0.8 142,567 - IAI47_00660 0.46 -1.1 142,567 - IAI47_00660 0.46 +0.6 142,567 - IAI47_00660 0.46 +0.5 142,567 - IAI47_00660 0.46 +1.5 142,567 - IAI47_00660 0.46 -1.5 142,567 - IAI47_00660 0.46 -1.9 142,567 - IAI47_00660 0.46 +5.4 142,567 - IAI47_00660 0.46 +1.5 142,567 - IAI47_00660 0.46 -0.6 142,629 + IAI47_00660 0.51 +0.2 142,629 + IAI47_00660 0.51 +0.5 142,629 + IAI47_00660 0.51 -1.9 142,630 - IAI47_00660 0.51 +3.6 142,630 - IAI47_00660 0.51 -1.2 142,652 - IAI47_00660 0.53 -2.1 142,661 - IAI47_00660 0.54 +0.3 142,661 - IAI47_00660 0.54 -2.2 142,687 + IAI47_00660 0.57 +0.1 142,687 + IAI47_00660 0.57 -2.2 142,687 + IAI47_00660 0.57 -1.9 142,687 + IAI47_00660 0.57 -1.8 142,687 + IAI47_00660 0.57 -0.8 142,687 + IAI47_00660 0.57 +1.6 142,688 - IAI47_00660 0.57 +0.3 142,688 - IAI47_00660 0.57 +2.8 142,688 - IAI47_00660 0.57 -0.3 142,688 - IAI47_00660 0.57 +0.7 142,688 - IAI47_00660 0.57 +1.2 142,688 - IAI47_00660 0.57 +1.7 142,688 - IAI47_00660 0.57 -1.3 142,688 - IAI47_00660 0.57 +0.8 142,688 - IAI47_00660 0.57 +0.4 142,688 - IAI47_00660 0.57 -3.2 142,688 - IAI47_00660 0.57 +1.7 142,703 - IAI47_00660 0.58 -2.6 142,703 - IAI47_00660 0.58 +1.0 142,720 + IAI47_00660 0.60 +1.6 142,721 - IAI47_00660 0.60 +1.3 142,721 - IAI47_00660 0.60 -2.5 142,721 - IAI47_00660 0.60 -0.6 142,721 - IAI47_00660 0.60 +0.2 142,721 - IAI47_00660 0.60 -0.5 142,721 - IAI47_00660 0.60 +0.7 142,721 - IAI47_00660 0.60 +0.2 142,721 - IAI47_00660 0.60 -1.8 142,721 - IAI47_00660 0.60 -0.6 142,721 - IAI47_00660 0.60 -1.2 142,721 - IAI47_00660 0.60 +0.7 142,721 - IAI47_00660 0.60 +1.1 142,721 - IAI47_00660 0.60 -0.8 142,741 + IAI47_00660 0.62 +2.3 142,742 - IAI47_00660 0.62 +3.0 142,779 + IAI47_00660 0.65 -0.3 142,854 - IAI47_00660 0.72 +3.4 142,854 - IAI47_00660 0.72 +0.9 142,866 + IAI47_00660 0.73 +0.2 142,867 - IAI47_00660 0.73 -1.2 142,867 - IAI47_00660 0.73 -0.2 142,867 - IAI47_00660 0.73 +0.5 142,867 - IAI47_00660 0.73 +0.8 142,867 - IAI47_00660 0.73 +1.9 142,893 - IAI47_00660 0.76 +2.1 142,916 - IAI47_00660 0.78 -0.6 143,005 + IAI47_00660 0.86 -1.4 143,005 + IAI47_00660 0.86 +0.4 143,006 - IAI47_00660 0.86 +2.5 143,007 + IAI47_00660 0.86 -1.8 143,007 + IAI47_00660 0.86 -1.5 143,008 - IAI47_00660 0.87 -0.8
Or see this region's nucleotide sequence