Experiment: ALP with Cobalt chloride 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_3221 and Dsui_3222 are separated by 39 nucleotides Dsui_3222 and Dsui_3223 are separated by 140 nucleotides Dsui_3223 and Dsui_3224 are separated by 11 nucleotides Dsui_3224 and Dsui_3225 overlap by 4 nucleotides
Dsui_3221: Dsui_3221 - cytochrome c553, at 3,460,034 to 3,460,720
_3221
Dsui_3222: Dsui_3222 - oligoribonuclease (3'->5' exoribonuclease), at 3,460,760 to 3,461,305
_3222
Dsui_3223: Dsui_3223 - Zn-dependent protease with chaperone function, at 3,461,446 to 3,462,717
_3223
Dsui_3224: Dsui_3224 - pterin-4a-carbinolamine dehydratase, at 3,462,729 to 3,463,064
_3224
Dsui_3225: Dsui_3225 - ribosome small subunit-dependent GTPase A, at 3,463,061 to 3,463,948
_3225
Position (kb)
3461
3462
3463 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3460.498 kb on - strand, within Dsui_3221 at 3460.660 kb on + strand at 3460.673 kb on + strand at 3460.673 kb on + strand at 3460.680 kb on + strand at 3460.688 kb on - strand at 3460.722 kb on + strand at 3460.726 kb on + strand at 3461.345 kb on + strand at 3461.353 kb on - strand at 3461.356 kb on + strand at 3461.393 kb on + strand at 3461.394 kb on + strand at 3461.480 kb on + strand at 3461.484 kb on - strand at 3461.522 kb on - strand at 3461.537 kb on - strand at 3461.580 kb on + strand, within Dsui_3223 at 3461.617 kb on + strand, within Dsui_3223 at 3461.657 kb on + strand, within Dsui_3223 at 3461.672 kb on + strand, within Dsui_3223 at 3461.750 kb on + strand, within Dsui_3223 at 3461.758 kb on - strand, within Dsui_3223 at 3461.797 kb on - strand, within Dsui_3223 at 3461.858 kb on + strand, within Dsui_3223 at 3461.858 kb on + strand, within Dsui_3223 at 3461.863 kb on + strand, within Dsui_3223 at 3461.918 kb on + strand, within Dsui_3223 at 3461.962 kb on - strand at 3461.978 kb on + strand, within Dsui_3223 at 3461.986 kb on - strand at 3462.152 kb on + strand, within Dsui_3223 at 3462.219 kb on + strand, within Dsui_3223 at 3462.249 kb on + strand, within Dsui_3223 at 3462.280 kb on - strand, within Dsui_3223 at 3462.286 kb on - strand at 3462.287 kb on + strand, within Dsui_3223 at 3462.319 kb on + strand, within Dsui_3223 at 3462.327 kb on - strand, within Dsui_3223 at 3462.332 kb on - strand, within Dsui_3223 at 3462.366 kb on + strand, within Dsui_3223 at 3462.409 kb on + strand, within Dsui_3223 at 3462.427 kb on + strand, within Dsui_3223 at 3462.464 kb on + strand, within Dsui_3223 at 3462.517 kb on + strand, within Dsui_3223 at 3462.535 kb on + strand, within Dsui_3223 at 3462.556 kb on - strand, within Dsui_3223 at 3462.563 kb on - strand, within Dsui_3223 at 3462.570 kb on + strand, within Dsui_3223 at 3462.605 kb on + strand at 3462.605 kb on + strand at 3462.605 kb on + strand at 3462.687 kb on + strand at 3462.698 kb on + strand at 3462.720 kb on - strand at 3462.740 kb on - strand at 3462.768 kb on - strand, within Dsui_3224 at 3462.962 kb on + strand, within Dsui_3224 at 3462.965 kb on - strand, within Dsui_3224 at 3462.970 kb on - strand, within Dsui_3224 at 3463.072 kb on + strand at 3463.079 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cobalt chloride 0.2 mM remove 3,460,498 - Dsui_3221 0.68 -0.1 3,460,660 + +0.6 3,460,673 + -1.4 3,460,673 + -0.9 3,460,680 + -1.7 3,460,688 - -0.1 3,460,722 + +0.6 3,460,726 + +0.6 3,461,345 + -1.2 3,461,353 - +0.6 3,461,356 + -0.7 3,461,393 + -1.1 3,461,394 + -1.1 3,461,480 + -1.7 3,461,484 - -1.4 3,461,522 - +0.6 3,461,537 - -0.4 3,461,580 + Dsui_3223 0.11 -0.5 3,461,617 + Dsui_3223 0.13 -0.8 3,461,657 + Dsui_3223 0.17 -0.7 3,461,672 + Dsui_3223 0.18 -0.6 3,461,750 + Dsui_3223 0.24 -1.6 3,461,758 - Dsui_3223 0.25 +0.6 3,461,797 - Dsui_3223 0.28 -1.4 3,461,858 + Dsui_3223 0.32 -0.9 3,461,858 + Dsui_3223 0.32 -1.5 3,461,863 + Dsui_3223 0.33 -1.2 3,461,918 + Dsui_3223 0.37 -1.8 3,461,962 - +0.6 3,461,978 + Dsui_3223 0.42 -1.8 3,461,986 - +0.6 3,462,152 + Dsui_3223 0.56 -0.4 3,462,219 + Dsui_3223 0.61 -1.7 3,462,249 + Dsui_3223 0.63 -1.2 3,462,280 - Dsui_3223 0.66 +0.6 3,462,286 - +0.6 3,462,287 + Dsui_3223 0.66 -0.6 3,462,319 + Dsui_3223 0.69 -2.5 3,462,327 - Dsui_3223 0.69 -1.7 3,462,332 - Dsui_3223 0.70 -0.4 3,462,366 + Dsui_3223 0.72 +0.6 3,462,409 + Dsui_3223 0.76 -1.0 3,462,427 + Dsui_3223 0.77 -0.3 3,462,464 + Dsui_3223 0.80 -0.4 3,462,517 + Dsui_3223 0.84 -0.8 3,462,535 + Dsui_3223 0.86 -0.6 3,462,556 - Dsui_3223 0.87 -0.3 3,462,563 - Dsui_3223 0.88 -1.3 3,462,570 + Dsui_3223 0.88 -0.4 3,462,605 + -0.5 3,462,605 + -0.7 3,462,605 + -0.9 3,462,687 + -0.9 3,462,698 + -0.3 3,462,720 - +0.7 3,462,740 - -0.3 3,462,768 - Dsui_3224 0.12 +0.7 3,462,962 + Dsui_3224 0.69 -1.3 3,462,965 - Dsui_3224 0.70 +0.7 3,462,970 - Dsui_3224 0.72 -0.9 3,463,072 + -0.4 3,463,079 + -3.7
Or see this region's nucleotide sequence