Experiment: ALP with Cobalt chloride 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_1021 and Dsui_1022 are separated by 208 nucleotides Dsui_1022 and Dsui_1023 overlap by 4 nucleotides Dsui_1023 and Dsui_1024 are separated by 13 nucleotides
Dsui_1021: Dsui_1021 - NAD(FAD)-dependent dehydrogenase, at 1,053,184 to 1,054,518
_1021
Dsui_1022: Dsui_1022 - cell division protein, at 1,054,727 to 1,055,632
_1022
Dsui_1023: Dsui_1023 - putative ATPase involved in cell division, at 1,055,629 to 1,056,309
_1023
Dsui_1024: Dsui_1024 - signal recognition particle-docking protein FtsY, at 1,056,323 to 1,057,327
_1024
Position (kb)
1054
1055
1056 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1053.821 kb on - strand, within Dsui_1021 at 1053.863 kb on - strand, within Dsui_1021 at 1053.893 kb on - strand, within Dsui_1021 at 1053.989 kb on + strand, within Dsui_1021 at 1053.997 kb on - strand, within Dsui_1021 at 1054.030 kb on + strand, within Dsui_1021 at 1054.061 kb on + strand, within Dsui_1021 at 1054.134 kb on + strand, within Dsui_1021 at 1054.276 kb on - strand, within Dsui_1021 at 1054.300 kb on - strand, within Dsui_1021 at 1054.418 kb on - strand at 1054.464 kb on - strand at 1054.527 kb on + strand at 1054.598 kb on + strand at 1054.611 kb on - strand at 1054.628 kb on + strand at 1054.750 kb on + strand at 1054.758 kb on - strand at 1054.761 kb on + strand at 1054.764 kb on - strand at 1054.764 kb on - strand at 1054.774 kb on - strand at 1054.818 kb on - strand, within Dsui_1022 at 1055.044 kb on - strand, within Dsui_1022 at 1055.167 kb on - strand, within Dsui_1022 at 1055.193 kb on - strand, within Dsui_1022 at 1055.193 kb on - strand, within Dsui_1022 at 1055.235 kb on - strand, within Dsui_1022 at 1055.442 kb on - strand, within Dsui_1022 at 1055.487 kb on - strand, within Dsui_1022 at 1055.527 kb on - strand, within Dsui_1022 at 1055.556 kb on + strand at 1055.564 kb on - strand at 1055.639 kb on + strand at 1055.765 kb on + strand, within Dsui_1023 at 1055.765 kb on + strand, within Dsui_1023 at 1055.765 kb on + strand, within Dsui_1023 at 1055.765 kb on + strand, within Dsui_1023 at 1055.773 kb on - strand, within Dsui_1023 at 1055.773 kb on - strand, within Dsui_1023 at 1055.778 kb on - strand, within Dsui_1023 at 1055.848 kb on + strand, within Dsui_1023 at 1055.856 kb on - strand, within Dsui_1023 at 1055.883 kb on - strand, within Dsui_1023 at 1055.912 kb on + strand, within Dsui_1023 at 1055.936 kb on + strand, within Dsui_1023 at 1055.941 kb on + strand, within Dsui_1023 at 1055.973 kb on + strand, within Dsui_1023 at 1056.004 kb on - strand, within Dsui_1023 at 1056.073 kb on + strand, within Dsui_1023 at 1056.124 kb on + strand, within Dsui_1023 at 1056.129 kb on + strand at 1056.139 kb on + strand, within Dsui_1023 at 1056.170 kb on + strand, within Dsui_1023 at 1056.188 kb on - strand, within Dsui_1023 at 1056.261 kb on - strand at 1056.283 kb on + strand at 1056.283 kb on - strand at 1056.291 kb on - strand at 1056.314 kb on + strand at 1056.348 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cobalt chloride 0.2 mM remove 1,053,821 - Dsui_1021 0.48 -0.4 1,053,863 - Dsui_1021 0.51 -0.6 1,053,893 - Dsui_1021 0.53 -0.7 1,053,989 + Dsui_1021 0.60 -2.7 1,053,997 - Dsui_1021 0.61 -0.0 1,054,030 + Dsui_1021 0.63 -1.1 1,054,061 + Dsui_1021 0.66 +0.2 1,054,134 + Dsui_1021 0.71 +0.8 1,054,276 - Dsui_1021 0.82 +0.2 1,054,300 - Dsui_1021 0.84 -0.3 1,054,418 - -0.3 1,054,464 - -0.2 1,054,527 + +0.3 1,054,598 + -1.5 1,054,611 - -0.3 1,054,628 + -0.2 1,054,750 + +0.8 1,054,758 - +0.0 1,054,761 + -1.2 1,054,764 - -0.7 1,054,764 - -0.3 1,054,774 - -0.2 1,054,818 - Dsui_1022 0.10 -0.3 1,055,044 - Dsui_1022 0.35 -0.6 1,055,167 - Dsui_1022 0.49 -0.2 1,055,193 - Dsui_1022 0.51 -0.0 1,055,193 - Dsui_1022 0.51 +0.1 1,055,235 - Dsui_1022 0.56 -0.4 1,055,442 - Dsui_1022 0.79 +0.4 1,055,487 - Dsui_1022 0.84 +0.8 1,055,527 - Dsui_1022 0.88 -0.4 1,055,556 + -0.8 1,055,564 - -0.2 1,055,639 + -0.2 1,055,765 + Dsui_1023 0.20 +0.8 1,055,765 + Dsui_1023 0.20 -0.2 1,055,765 + Dsui_1023 0.20 -0.2 1,055,765 + Dsui_1023 0.20 -0.8 1,055,773 - Dsui_1023 0.21 -0.3 1,055,773 - Dsui_1023 0.21 -0.1 1,055,778 - Dsui_1023 0.22 -0.3 1,055,848 + Dsui_1023 0.32 -0.8 1,055,856 - Dsui_1023 0.33 +0.2 1,055,883 - Dsui_1023 0.37 -0.4 1,055,912 + Dsui_1023 0.42 -1.8 1,055,936 + Dsui_1023 0.45 +0.8 1,055,941 + Dsui_1023 0.46 -0.2 1,055,973 + Dsui_1023 0.51 -0.8 1,056,004 - Dsui_1023 0.55 -0.3 1,056,073 + Dsui_1023 0.65 -0.8 1,056,124 + Dsui_1023 0.73 +0.8 1,056,129 + +0.8 1,056,139 + Dsui_1023 0.75 -0.8 1,056,170 + Dsui_1023 0.79 -3.9 1,056,188 - Dsui_1023 0.82 +0.1 1,056,261 - -0.1 1,056,283 + -0.8 1,056,283 - -0.7 1,056,291 - +0.2 1,056,314 + -0.2 1,056,348 - +0.1
Or see this region's nucleotide sequence