Experiment: ALP with Cobalt chloride 0.2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dsui_0501 and Dsui_0502 are separated by 34 nucleotides Dsui_0502 and Dsui_0503 are separated by 46 nucleotides Dsui_0503 and Dsui_0504 overlap by 4 nucleotides Dsui_0504 and Dsui_0505 are separated by 55 nucleotides
Dsui_0501: Dsui_0501 - glycine/D-amino acid oxidase, deaminating, at 503,490 to 504,740
_0501
Dsui_0502: Dsui_0502 - enoyl-CoA hydratase/carnithine racemase, at 504,775 to 505,566
_0502
Dsui_0503: Dsui_0503 - Mg-dependent DNase, at 505,613 to 506,473
_0503
Dsui_0504: Dsui_0504 - putative membrane protein, at 506,470 to 507,375
_0504
Dsui_0505: Dsui_0505 - 7-cyano-7-deazaguanine reductase, at 507,431 to 508,264
_0505
Position (kb) 505
506
507 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 504.780 kb on - strand at 504.780 kb on - strand at 504.808 kb on + strand at 504.828 kb on - strand at 504.830 kb on + strand at 504.945 kb on + strand, within Dsui_0502 at 505.044 kb on + strand, within Dsui_0502 at 505.052 kb on - strand, within Dsui_0502 at 505.060 kb on - strand, within Dsui_0502 at 505.244 kb on + strand, within Dsui_0502 at 505.325 kb on - strand, within Dsui_0502 at 505.340 kb on - strand at 505.407 kb on - strand, within Dsui_0502 at 505.410 kb on + strand, within Dsui_0502 at 505.410 kb on + strand, within Dsui_0502 at 505.618 kb on + strand at 505.713 kb on - strand at 505.713 kb on - strand at 505.810 kb on - strand at 505.810 kb on - strand at 505.875 kb on + strand, within Dsui_0503 at 505.914 kb on + strand, within Dsui_0503 at 505.966 kb on + strand, within Dsui_0503 at 505.989 kb on - strand, within Dsui_0503 at 506.024 kb on - strand, within Dsui_0503 at 506.101 kb on - strand, within Dsui_0503 at 506.134 kb on + strand, within Dsui_0503 at 506.456 kb on - strand at 506.530 kb on - strand at 506.618 kb on - strand at 506.618 kb on - strand, within Dsui_0504 at 506.636 kb on + strand, within Dsui_0504 at 506.644 kb on - strand, within Dsui_0504 at 506.655 kb on + strand, within Dsui_0504 at 506.713 kb on - strand at 506.713 kb on - strand at 506.746 kb on - strand, within Dsui_0504 at 506.826 kb on - strand, within Dsui_0504 at 506.964 kb on + strand, within Dsui_0504 at 506.972 kb on - strand, within Dsui_0504 at 507.005 kb on + strand, within Dsui_0504 at 507.038 kb on + strand, within Dsui_0504 at 507.084 kb on - strand, within Dsui_0504 at 507.150 kb on + strand, within Dsui_0504 at 507.176 kb on - strand, within Dsui_0504 at 507.221 kb on + strand, within Dsui_0504 at 507.254 kb on - strand at 507.351 kb on + strand at 507.352 kb on + strand at 507.357 kb on + strand at 507.365 kb on - strand at 507.369 kb on + strand at 507.369 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction ALP with Cobalt chloride 0.2 mM remove 504,780 - -1.0 504,780 - +0.6 504,808 + -0.5 504,828 - -0.5 504,830 + -0.5 504,945 + Dsui_0502 0.21 -0.4 505,044 + Dsui_0502 0.34 -0.2 505,052 - Dsui_0502 0.35 +0.6 505,060 - Dsui_0502 0.36 +0.0 505,244 + Dsui_0502 0.59 -0.3 505,325 - Dsui_0502 0.69 -3.0 505,340 - +0.6 505,407 - Dsui_0502 0.80 -1.0 505,410 + Dsui_0502 0.80 -0.2 505,410 + Dsui_0502 0.80 -0.9 505,618 + -0.1 505,713 - -0.4 505,713 - +0.6 505,810 - +0.6 505,810 - +0.6 505,875 + Dsui_0503 0.30 -0.6 505,914 + Dsui_0503 0.35 -0.3 505,966 + Dsui_0503 0.41 -0.8 505,989 - Dsui_0503 0.44 -1.0 506,024 - Dsui_0503 0.48 -1.4 506,101 - Dsui_0503 0.57 -1.0 506,134 + Dsui_0503 0.61 +1.2 506,456 - -2.2 506,530 - +0.6 506,618 - +0.6 506,618 - Dsui_0504 0.16 +0.6 506,636 + Dsui_0504 0.18 -0.4 506,644 - Dsui_0504 0.19 -2.0 506,655 + Dsui_0504 0.20 +1.9 506,713 - +0.6 506,713 - +0.6 506,746 - Dsui_0504 0.30 -0.4 506,826 - Dsui_0504 0.39 -1.4 506,964 + Dsui_0504 0.55 -0.8 506,972 - Dsui_0504 0.55 +0.6 507,005 + Dsui_0504 0.59 -0.1 507,038 + Dsui_0504 0.63 -0.4 507,084 - Dsui_0504 0.68 -2.4 507,150 + Dsui_0504 0.75 -2.1 507,176 - Dsui_0504 0.78 +0.6 507,221 + Dsui_0504 0.83 -1.4 507,254 - +0.6 507,351 + -0.8 507,352 + -0.9 507,357 + -1.2 507,365 - -2.4 507,369 + -1.0 507,369 + -1.9
Or see this region's nucleotide sequence