Experiment: L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27420 and BWI76_RS27425 are separated by 54 nucleotides BWI76_RS27425 and BWI76_RS27430 are separated by 18 nucleotides BWI76_RS27430 and BWI76_RS27435 are separated by 4 nucleotides BWI76_RS27435 and BWI76_RS27440 are separated by 2 nucleotides
BWI76_RS27420: BWI76_RS27420 - guanylate kinase, at 5,688,975 to 5,689,598
_RS27420
BWI76_RS27425: BWI76_RS27425 - DNA-directed RNA polymerase subunit omega, at 5,689,653 to 5,689,928
_RS27425
BWI76_RS27430: BWI76_RS27430 - (p)ppGpp synthetase, at 5,689,947 to 5,692,067
_RS27430
BWI76_RS27435: BWI76_RS27435 - tRNA (guanosine(18)-2'-O)-methyltransferase TrmH, at 5,692,072 to 5,692,761
_RS27435
BWI76_RS27440: BWI76_RS27440 - DNA helicase RecG, at 5,692,764 to 5,694,845
_RS27440
Position (kb) 5689
5690
5691
5692
5693 Strain fitness (log2 ratio) -2
-1
0
1 at 5689.709 kb on + strand, within BWI76_RS27425 at 5689.821 kb on + strand, within BWI76_RS27425 at 5689.821 kb on + strand, within BWI76_RS27425 at 5691.447 kb on - strand, within BWI76_RS27430 at 5691.721 kb on - strand, within BWI76_RS27430 at 5691.896 kb on + strand at 5692.004 kb on - strand at 5692.139 kb on + strand at 5692.287 kb on + strand, within BWI76_RS27435 at 5692.295 kb on - strand, within BWI76_RS27435 at 5692.295 kb on - strand, within BWI76_RS27435 at 5692.333 kb on + strand, within BWI76_RS27435 at 5692.333 kb on + strand, within BWI76_RS27435 at 5692.420 kb on - strand, within BWI76_RS27435 at 5692.471 kb on + strand, within BWI76_RS27435 at 5692.545 kb on + strand, within BWI76_RS27435 at 5692.553 kb on - strand, within BWI76_RS27435 at 5692.605 kb on + strand, within BWI76_RS27435 at 5692.678 kb on + strand, within BWI76_RS27435 at 5692.868 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate (C) remove 5,689,709 + BWI76_RS27425 0.20 -0.1 5,689,821 + BWI76_RS27425 0.61 -0.3 5,689,821 + BWI76_RS27425 0.61 -0.7 5,691,447 - BWI76_RS27430 0.71 -2.1 5,691,721 - BWI76_RS27430 0.84 -2.1 5,691,896 + -0.5 5,692,004 - -0.1 5,692,139 + +0.2 5,692,287 + BWI76_RS27435 0.31 +1.0 5,692,295 - BWI76_RS27435 0.32 -1.2 5,692,295 - BWI76_RS27435 0.32 -1.7 5,692,333 + BWI76_RS27435 0.38 -0.7 5,692,333 + BWI76_RS27435 0.38 -0.6 5,692,420 - BWI76_RS27435 0.50 -1.5 5,692,471 + BWI76_RS27435 0.58 -0.3 5,692,545 + BWI76_RS27435 0.69 -0.1 5,692,553 - BWI76_RS27435 0.70 -1.2 5,692,605 + BWI76_RS27435 0.77 +0.0 5,692,678 + BWI76_RS27435 0.88 -1.5 5,692,868 - -0.6
Or see this region's nucleotide sequence