Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22435

Experiment: L-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22425 and BWI76_RS22430 overlap by 4 nucleotidesBWI76_RS22430 and BWI76_RS22435 overlap by 4 nucleotidesBWI76_RS22435 and BWI76_RS22440 overlap by 7 nucleotidesBWI76_RS22440 and BWI76_RS22445 are separated by 124 nucleotides BWI76_RS22425: BWI76_RS22425 - metal ABC transporter substrate-binding protein, at 4,676,159 to 4,677,076 _RS22425 BWI76_RS22430: BWI76_RS22430 - manganese/iron transporter ATP-binding protein, at 4,677,073 to 4,677,894 _RS22430 BWI76_RS22435: BWI76_RS22435 - iron ABC transporter permease AfeC, at 4,677,891 to 4,678,739 _RS22435 BWI76_RS22440: BWI76_RS22440 - iron ABC transporter permease, at 4,678,733 to 4,679,587 _RS22440 BWI76_RS22445: BWI76_RS22445 - hypothetical protein, at 4,679,712 to 4,680,086 _RS22445 Position (kb) 4677 4678 4679Strain fitness (log2 ratio) -1 0 1at 4677.107 kb on + strandat 4677.363 kb on + strand, within BWI76_RS22430at 4677.648 kb on + strand, within BWI76_RS22430at 4677.811 kb on + strand, within BWI76_RS22430at 4677.819 kb on - strandat 4678.036 kb on + strand, within BWI76_RS22435at 4678.079 kb on - strand, within BWI76_RS22435at 4678.174 kb on + strand, within BWI76_RS22435at 4678.296 kb on - strand, within BWI76_RS22435at 4678.351 kb on + strand, within BWI76_RS22435at 4678.516 kb on - strand, within BWI76_RS22435at 4678.561 kb on - strand, within BWI76_RS22435at 4678.747 kb on + strandat 4678.747 kb on + strandat 4679.211 kb on + strand, within BWI76_RS22440at 4679.272 kb on + strand, within BWI76_RS22440at 4679.366 kb on - strand, within BWI76_RS22440at 4679.425 kb on - strand, within BWI76_RS22440at 4679.489 kb on + strand, within BWI76_RS22440at 4679.642 kb on - strandat 4679.680 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Lactate (C)
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4,677,107 + -1.1
4,677,363 + BWI76_RS22430 0.35 -0.5
4,677,648 + BWI76_RS22430 0.70 -0.5
4,677,811 + BWI76_RS22430 0.90 +0.5
4,677,819 - +0.5
4,678,036 + BWI76_RS22435 0.17 -1.5
4,678,079 - BWI76_RS22435 0.22 -0.7
4,678,174 + BWI76_RS22435 0.33 -1.2
4,678,296 - BWI76_RS22435 0.48 +0.0
4,678,351 + BWI76_RS22435 0.54 -0.1
4,678,516 - BWI76_RS22435 0.74 -1.1
4,678,561 - BWI76_RS22435 0.79 +0.1
4,678,747 + +0.1
4,678,747 + -0.2
4,679,211 + BWI76_RS22440 0.56 +0.0
4,679,272 + BWI76_RS22440 0.63 -1.1
4,679,366 - BWI76_RS22440 0.74 +0.6
4,679,425 - BWI76_RS22440 0.81 -0.0
4,679,489 + BWI76_RS22440 0.88 +0.6
4,679,642 - +0.7
4,679,680 - -0.7

Or see this region's nucleotide sequence