Experiment: Carbon source Sucrose 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0355 and Shewana3_0356 are separated by 590 nucleotides Shewana3_0356 and ilvM overlap by 4 nucleotides ilvM and Shewana3_0358 are separated by 79 nucleotides
Shewana3_0355: Shewana3_0355 - ketol-acid reductoisomerase (RefSeq), at 390,117 to 391,595
_0355
Shewana3_0356: Shewana3_0356 - acetolactate synthase 2 catalytic subunit (RefSeq), at 392,186 to 393,859
_0356
Shewana3_0357: ilvM - acetolactate synthase 2 regulatory subunit (RefSeq), at 393,856 to 394,116
ilvM
Shewana3_0358: Shewana3_0358 - dihydroxy-acid dehydratase (RefSeq), at 394,196 to 396,055
_0358
Position (kb) 392
393
394 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 391.216 kb on - strand, within Shewana3_0355 at 391.348 kb on - strand, within Shewana3_0355 at 391.354 kb on + strand, within Shewana3_0355 at 391.363 kb on + strand, within Shewana3_0355 at 391.383 kb on - strand, within Shewana3_0355 at 391.385 kb on + strand, within Shewana3_0355 at 391.442 kb on + strand, within Shewana3_0355 at 391.442 kb on + strand, within Shewana3_0355 at 391.463 kb on - strand at 391.525 kb on + strand at 391.849 kb on - strand at 392.295 kb on + strand at 392.295 kb on + strand at 392.327 kb on + strand at 392.512 kb on + strand, within Shewana3_0356 at 392.573 kb on - strand, within Shewana3_0356 at 392.585 kb on - strand, within Shewana3_0356 at 392.605 kb on + strand, within Shewana3_0356 at 392.639 kb on + strand, within Shewana3_0356 at 392.655 kb on + strand, within Shewana3_0356 at 392.663 kb on - strand, within Shewana3_0356 at 392.668 kb on - strand, within Shewana3_0356 at 392.681 kb on - strand, within Shewana3_0356 at 392.736 kb on + strand, within Shewana3_0356 at 392.753 kb on + strand, within Shewana3_0356 at 392.804 kb on - strand, within Shewana3_0356 at 392.899 kb on + strand, within Shewana3_0356 at 392.973 kb on + strand, within Shewana3_0356 at 392.981 kb on + strand, within Shewana3_0356 at 393.181 kb on - strand, within Shewana3_0356 at 393.298 kb on + strand, within Shewana3_0356 at 393.343 kb on - strand, within Shewana3_0356 at 393.359 kb on + strand, within Shewana3_0356 at 393.391 kb on - strand, within Shewana3_0356 at 393.398 kb on + strand, within Shewana3_0356 at 393.406 kb on - strand, within Shewana3_0356 at 393.406 kb on - strand, within Shewana3_0356 at 393.419 kb on + strand, within Shewana3_0356 at 393.530 kb on - strand, within Shewana3_0356 at 393.672 kb on - strand, within Shewana3_0356 at 393.719 kb on - strand at 393.880 kb on - strand at 393.943 kb on - strand, within ilvM at 394.024 kb on - strand, within ilvM at 394.071 kb on + strand, within ilvM at 394.120 kb on - strand at 394.133 kb on + strand at 394.152 kb on + strand at 394.184 kb on + strand at 394.473 kb on - strand, within Shewana3_0358 at 394.503 kb on + strand, within Shewana3_0358 at 394.643 kb on - strand, within Shewana3_0358 at 394.755 kb on + strand, within Shewana3_0358 at 394.858 kb on + strand, within Shewana3_0358
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source Sucrose 10 mM remove 391,216 - Shewana3_0355 0.74 -4.4 391,348 - Shewana3_0355 0.83 -3.0 391,354 + Shewana3_0355 0.84 -3.8 391,363 + Shewana3_0355 0.84 -4.4 391,383 - Shewana3_0355 0.86 -3.1 391,385 + Shewana3_0355 0.86 -1.3 391,442 + Shewana3_0355 0.90 -4.3 391,442 + Shewana3_0355 0.90 -4.8 391,463 - -3.9 391,525 + -3.0 391,849 - -4.2 392,295 + -4.0 392,295 + -4.4 392,327 + -4.6 392,512 + Shewana3_0356 0.19 -3.2 392,573 - Shewana3_0356 0.23 -0.9 392,585 - Shewana3_0356 0.24 -5.0 392,605 + Shewana3_0356 0.25 -4.4 392,639 + Shewana3_0356 0.27 -2.8 392,655 + Shewana3_0356 0.28 -2.6 392,663 - Shewana3_0356 0.28 -4.5 392,668 - Shewana3_0356 0.29 -1.3 392,681 - Shewana3_0356 0.30 -3.7 392,736 + Shewana3_0356 0.33 +0.9 392,753 + Shewana3_0356 0.34 -0.3 392,804 - Shewana3_0356 0.37 -3.2 392,899 + Shewana3_0356 0.43 -5.5 392,973 + Shewana3_0356 0.47 -3.4 392,981 + Shewana3_0356 0.47 -3.4 393,181 - Shewana3_0356 0.59 -3.6 393,298 + Shewana3_0356 0.66 -1.8 393,343 - Shewana3_0356 0.69 -4.2 393,359 + Shewana3_0356 0.70 -0.8 393,391 - Shewana3_0356 0.72 -3.6 393,398 + Shewana3_0356 0.72 -0.7 393,406 - Shewana3_0356 0.73 -3.4 393,406 - Shewana3_0356 0.73 -3.2 393,419 + Shewana3_0356 0.74 +0.1 393,530 - Shewana3_0356 0.80 -5.4 393,672 - Shewana3_0356 0.89 -3.5 393,719 - -5.3 393,880 - -1.1 393,943 - ilvM Shewana3_0357 0.33 -3.2 394,024 - ilvM Shewana3_0357 0.64 -3.1 394,071 + ilvM Shewana3_0357 0.82 -0.3 394,120 - -2.9 394,133 + +0.1 394,152 + -1.4 394,184 + -1.0 394,473 - Shewana3_0358 0.15 -4.2 394,503 + Shewana3_0358 0.17 -4.3 394,643 - Shewana3_0358 0.24 -4.3 394,755 + Shewana3_0358 0.30 -3.2 394,858 + Shewana3_0358 0.36 -1.6
Or see this region's nucleotide sequence