Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35290 and RR42_RS35295 are separated by 242 nucleotides RR42_RS35295 and RR42_RS35300 are separated by 47 nucleotides
RR42_RS35290: RR42_RS35290 - Fe-S oxidoreductase, at 3,372,281 to 3,373,375
_RS35290
RR42_RS35295: RR42_RS35295 - diguanylate cyclase, at 3,373,618 to 3,374,316
_RS35295
RR42_RS35300: RR42_RS35300 - ABC transporter substrate-binding protein, at 3,374,364 to 3,375,338
_RS35300
Position (kb)
3373
3374
3375 Strain fitness (log2 ratio)
-2
-1
0
1 at 3372.874 kb on - strand, within RR42_RS35290 at 3372.933 kb on + strand, within RR42_RS35290 at 3372.933 kb on + strand, within RR42_RS35290 at 3373.323 kb on + strand at 3373.323 kb on + strand at 3373.323 kb on + strand at 3373.324 kb on - strand at 3373.393 kb on + strand at 3373.406 kb on - strand at 3373.414 kb on - strand at 3373.420 kb on + strand at 3373.420 kb on + strand at 3373.420 kb on + strand at 3373.421 kb on - strand at 3373.429 kb on + strand at 3373.429 kb on + strand at 3373.433 kb on + strand at 3373.433 kb on + strand at 3373.434 kb on - strand at 3373.434 kb on - strand at 3373.434 kb on - strand at 3373.441 kb on + strand at 3373.441 kb on + strand at 3373.441 kb on + strand at 3373.441 kb on + strand at 3373.441 kb on + strand at 3373.442 kb on - strand at 3373.442 kb on - strand at 3373.442 kb on - strand at 3373.454 kb on + strand at 3373.454 kb on + strand at 3373.455 kb on - strand at 3373.461 kb on + strand at 3373.461 kb on + strand at 3373.461 kb on + strand at 3373.462 kb on - strand at 3373.462 kb on - strand at 3373.482 kb on + strand at 3373.485 kb on + strand at 3373.486 kb on - strand at 3373.486 kb on - strand at 3373.540 kb on + strand at 3373.559 kb on + strand at 3373.613 kb on + strand at 3373.614 kb on - strand at 3373.914 kb on - strand, within RR42_RS35295 at 3373.914 kb on - strand, within RR42_RS35295 at 3374.290 kb on - strand at 3374.365 kb on + strand at 3374.365 kb on + strand at 3374.365 kb on + strand at 3374.701 kb on + strand, within RR42_RS35300 at 3374.785 kb on + strand, within RR42_RS35300 at 3374.786 kb on - strand, within RR42_RS35300 at 3374.786 kb on - strand, within RR42_RS35300 at 3374.957 kb on + strand, within RR42_RS35300 at 3374.957 kb on + strand, within RR42_RS35300 at 3374.957 kb on + strand, within RR42_RS35300 at 3374.957 kb on + strand, within RR42_RS35300 at 3374.958 kb on - strand, within RR42_RS35300 at 3374.958 kb on - strand, within RR42_RS35300 at 3374.959 kb on + strand, within RR42_RS35300 at 3374.959 kb on + strand, within RR42_RS35300 at 3374.960 kb on - strand, within RR42_RS35300 at 3374.960 kb on - strand, within RR42_RS35300 at 3374.960 kb on - strand, within RR42_RS35300 at 3374.960 kb on - strand, within RR42_RS35300 at 3375.009 kb on + strand, within RR42_RS35300 at 3375.010 kb on + strand, within RR42_RS35300 at 3375.010 kb on + strand, within RR42_RS35300
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 3,372,874 - RR42_RS35290 0.54 +0.5 3,372,933 + RR42_RS35290 0.60 +0.8 3,372,933 + RR42_RS35290 0.60 -0.0 3,373,323 + +0.2 3,373,323 + -0.4 3,373,323 + +0.4 3,373,324 - -0.8 3,373,393 + -0.8 3,373,406 - +0.7 3,373,414 - +0.2 3,373,420 + +0.3 3,373,420 + +0.7 3,373,420 + +0.2 3,373,421 - -0.5 3,373,429 + -0.4 3,373,429 + +0.9 3,373,433 + +0.1 3,373,433 + +0.3 3,373,434 - +0.5 3,373,434 - -0.2 3,373,434 - -0.5 3,373,441 + -0.3 3,373,441 + +0.0 3,373,441 + -0.1 3,373,441 + -1.1 3,373,441 + +0.5 3,373,442 - -1.8 3,373,442 - +0.1 3,373,442 - +0.7 3,373,454 + +0.7 3,373,454 + -1.1 3,373,455 - +1.3 3,373,461 + -0.4 3,373,461 + -0.3 3,373,461 + +0.7 3,373,462 - +0.4 3,373,462 - -0.3 3,373,482 + -0.1 3,373,485 + -0.7 3,373,486 - -0.5 3,373,486 - -0.5 3,373,540 + +0.5 3,373,559 + -0.1 3,373,613 + -0.2 3,373,614 - +0.4 3,373,914 - RR42_RS35295 0.42 -1.3 3,373,914 - RR42_RS35295 0.42 -0.5 3,374,290 - -0.1 3,374,365 + +0.2 3,374,365 + +0.9 3,374,365 + +0.3 3,374,701 + RR42_RS35300 0.35 +0.4 3,374,785 + RR42_RS35300 0.43 +0.3 3,374,786 - RR42_RS35300 0.43 -0.7 3,374,786 - RR42_RS35300 0.43 -0.4 3,374,957 + RR42_RS35300 0.61 +0.7 3,374,957 + RR42_RS35300 0.61 -0.5 3,374,957 + RR42_RS35300 0.61 +0.2 3,374,957 + RR42_RS35300 0.61 +0.1 3,374,958 - RR42_RS35300 0.61 +0.8 3,374,958 - RR42_RS35300 0.61 -0.0 3,374,959 + RR42_RS35300 0.61 -0.0 3,374,959 + RR42_RS35300 0.61 +0.6 3,374,960 - RR42_RS35300 0.61 +0.5 3,374,960 - RR42_RS35300 0.61 +0.2 3,374,960 - RR42_RS35300 0.61 +1.7 3,374,960 - RR42_RS35300 0.61 +0.3 3,375,009 + RR42_RS35300 0.66 +0.1 3,375,010 + RR42_RS35300 0.66 -0.6 3,375,010 + RR42_RS35300 0.66 +0.5
Or see this region's nucleotide sequence