Experiment: R2A with Dimethyl Sulfoxide 5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS35105 and RR42_RS35110 are separated by 88 nucleotides RR42_RS35110 and RR42_RS35115 are separated by 152 nucleotides RR42_RS35115 and RR42_RS35120 are separated by 52 nucleotides RR42_RS35120 and RR42_RS35125 are separated by 118 nucleotides
RR42_RS35105: RR42_RS35105 - hydrolase, at 3,328,330 to 3,329,418
_RS35105
RR42_RS35110: RR42_RS35110 - hypothetical protein, at 3,329,507 to 3,329,689
_RS35110
RR42_RS35115: RR42_RS35115 - riboflavin synthase subunit beta, at 3,329,842 to 3,330,312
_RS35115
RR42_RS35120: RR42_RS35120 - benzoyl-CoA oxygenase, at 3,330,365 to 3,331,645
_RS35120
RR42_RS35125: RR42_RS35125 - benzoyl-CoA oxygenase, at 3,331,764 to 3,333,191
_RS35125
Position (kb)
3330
3331
3332 Strain fitness (log2 ratio)
-1
0
1
2 at 3329.421 kb on + strand at 3329.515 kb on + strand at 3329.515 kb on + strand at 3329.515 kb on + strand at 3329.515 kb on + strand at 3329.516 kb on - strand at 3329.516 kb on - strand at 3329.516 kb on - strand at 3329.606 kb on + strand, within RR42_RS35110 at 3329.606 kb on + strand at 3329.607 kb on - strand, within RR42_RS35110 at 3329.689 kb on + strand at 3329.690 kb on - strand at 3330.094 kb on + strand, within RR42_RS35115 at 3330.094 kb on + strand, within RR42_RS35115 at 3330.095 kb on - strand, within RR42_RS35115 at 3330.370 kb on - strand at 3330.501 kb on + strand, within RR42_RS35120 at 3330.502 kb on - strand, within RR42_RS35120 at 3330.512 kb on - strand, within RR42_RS35120 at 3330.657 kb on + strand, within RR42_RS35120 at 3330.657 kb on + strand, within RR42_RS35120 at 3330.657 kb on + strand, within RR42_RS35120 at 3330.658 kb on - strand, within RR42_RS35120 at 3330.886 kb on + strand, within RR42_RS35120 at 3330.886 kb on + strand, within RR42_RS35120 at 3330.887 kb on - strand, within RR42_RS35120 at 3330.887 kb on - strand, within RR42_RS35120 at 3330.887 kb on - strand, within RR42_RS35120 at 3330.888 kb on + strand, within RR42_RS35120 at 3330.889 kb on - strand, within RR42_RS35120 at 3330.958 kb on + strand, within RR42_RS35120 at 3330.958 kb on + strand, within RR42_RS35120 at 3330.958 kb on + strand, within RR42_RS35120 at 3330.958 kb on + strand, within RR42_RS35120 at 3331.115 kb on - strand, within RR42_RS35120 at 3331.115 kb on - strand, within RR42_RS35120 at 3331.206 kb on + strand, within RR42_RS35120 at 3331.206 kb on + strand, within RR42_RS35120 at 3331.206 kb on + strand, within RR42_RS35120 at 3331.216 kb on + strand, within RR42_RS35120 at 3331.216 kb on + strand, within RR42_RS35120 at 3331.216 kb on + strand, within RR42_RS35120 at 3331.217 kb on - strand, within RR42_RS35120 at 3331.217 kb on - strand, within RR42_RS35120 at 3331.217 kb on - strand, within RR42_RS35120 at 3331.218 kb on + strand, within RR42_RS35120 at 3331.218 kb on + strand, within RR42_RS35120 at 3331.219 kb on - strand, within RR42_RS35120 at 3331.524 kb on + strand at 3331.524 kb on + strand at 3331.524 kb on + strand at 3331.524 kb on + strand at 3331.524 kb on + strand at 3331.524 kb on + strand at 3331.780 kb on + strand at 3331.883 kb on - strand at 3332.095 kb on + strand, within RR42_RS35125 at 3332.182 kb on + strand, within RR42_RS35125 at 3332.182 kb on + strand, within RR42_RS35125 at 3332.183 kb on - strand, within RR42_RS35125 at 3332.312 kb on - strand, within RR42_RS35125 at 3332.563 kb on + strand, within RR42_RS35125 at 3332.563 kb on + strand, within RR42_RS35125 at 3332.564 kb on - strand, within RR42_RS35125
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Dimethyl Sulfoxide 5 vol% remove 3,329,421 + -0.1 3,329,515 + +0.5 3,329,515 + -0.1 3,329,515 + -0.4 3,329,515 + +0.6 3,329,516 - -0.7 3,329,516 - +0.0 3,329,516 - +0.1 3,329,606 + RR42_RS35110 0.54 +0.3 3,329,606 + +0.1 3,329,607 - RR42_RS35110 0.55 +2.2 3,329,689 + -1.1 3,329,690 - -0.5 3,330,094 + RR42_RS35115 0.54 -0.5 3,330,094 + RR42_RS35115 0.54 -0.5 3,330,095 - RR42_RS35115 0.54 -0.2 3,330,370 - +1.1 3,330,501 + RR42_RS35120 0.11 -1.0 3,330,502 - RR42_RS35120 0.11 +0.1 3,330,512 - RR42_RS35120 0.11 +0.5 3,330,657 + RR42_RS35120 0.23 +0.0 3,330,657 + RR42_RS35120 0.23 -0.3 3,330,657 + RR42_RS35120 0.23 -1.5 3,330,658 - RR42_RS35120 0.23 +2.1 3,330,886 + RR42_RS35120 0.41 -0.2 3,330,886 + RR42_RS35120 0.41 -0.6 3,330,887 - RR42_RS35120 0.41 -0.3 3,330,887 - RR42_RS35120 0.41 -0.6 3,330,887 - RR42_RS35120 0.41 -0.3 3,330,888 + RR42_RS35120 0.41 +0.9 3,330,889 - RR42_RS35120 0.41 +0.5 3,330,958 + RR42_RS35120 0.46 -0.6 3,330,958 + RR42_RS35120 0.46 +0.0 3,330,958 + RR42_RS35120 0.46 -0.1 3,330,958 + RR42_RS35120 0.46 +0.1 3,331,115 - RR42_RS35120 0.59 +0.1 3,331,115 - RR42_RS35120 0.59 -0.6 3,331,206 + RR42_RS35120 0.66 -0.5 3,331,206 + RR42_RS35120 0.66 -1.1 3,331,206 + RR42_RS35120 0.66 -0.6 3,331,216 + RR42_RS35120 0.66 +0.1 3,331,216 + RR42_RS35120 0.66 +0.5 3,331,216 + RR42_RS35120 0.66 +0.1 3,331,217 - RR42_RS35120 0.67 -0.3 3,331,217 - RR42_RS35120 0.67 +0.1 3,331,217 - RR42_RS35120 0.67 -0.3 3,331,218 + RR42_RS35120 0.67 +0.3 3,331,218 + RR42_RS35120 0.67 -0.3 3,331,219 - RR42_RS35120 0.67 +0.5 3,331,524 + +0.4 3,331,524 + -0.6 3,331,524 + +1.4 3,331,524 + +1.0 3,331,524 + -1.4 3,331,524 + +0.4 3,331,780 + +0.1 3,331,883 - +0.3 3,332,095 + RR42_RS35125 0.23 +0.3 3,332,182 + RR42_RS35125 0.29 -0.2 3,332,182 + RR42_RS35125 0.29 -0.9 3,332,183 - RR42_RS35125 0.29 -0.6 3,332,312 - RR42_RS35125 0.38 +0.2 3,332,563 + RR42_RS35125 0.56 -0.3 3,332,563 + RR42_RS35125 0.56 +0.4 3,332,564 - RR42_RS35125 0.56 +0.5
Or see this region's nucleotide sequence